Biological preparation method of Hypericum perforatum L extractive

A technology for the biological preparation of Hypericum perforatum, applied in the field of medicine, can solve the problems of complex process, inconvenient purification, difficulty in using food and taking, etc., and achieve the effects of increasing hypericin content, easy industrial application, simple and feasible operation

Inactive Publication Date: 2010-09-15
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, the disadvantage of lye extraction is that the color of the obtained extract is poor, and a large amount of NaCl is entrained in the product, which brings a lot of inconvenience to the purification
[0006] 3. Organic solvent extraction-adsorption material enrichment and purification method: Chinese patent 200610078988.9 discloses an extraction method of hypericin: Hypericum perforatum is extracted with ethanol, the filtrate is concentrated under reduced pressure, and the concentrated solution is adsorbed with resin, eluted, collected The eluate is concentrated under reduced pressure to obtain a hypericin extract. The product obtained by this method contains higher hypericin, but the process is relatively complicated and the cost is high, and the resin used for adsorption is a chemically synthesized macromolecular compound , which contain unsynthesized chemical monomers, such as benzene, divinylbenzene, toluene, xylene and other organic residues, are not easy to be used for food and consumption, because the State Food and Drug Administration has restricted the separation and purification of macroporous adsorption resins. There are strict regulations on organic residues in health food and raw materials prepared with styrene-based resins. Therefore, the method of purifying Hypericum perforatum extract with macroporous adsorption resins is largely limited, and it is difficult to achieve industrialized mass production. Requirements, currently mainly still in the laboratory stage
[0007] Since the active ingredients in plants usually exist in plant cells, the use of biological enzymes to destroy the cell wall can promote the dissolution of active ingredients, thereby increasing the content of active ingredients in the extract and improving the utilization of medicinal materials, but so far no public reports have been seen. Application of enzymatic hydrolysis in the preparation of Hypericum perforatum extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] First dry Hypericum perforatum, crush it through a 20-mesh sieve, and form Hypericum perforatum powder. Weigh 1kg of Hypericum perforatum powder, then weigh 5g of cellulase, add 50g of warm water at 32°C to the cellulase, dissolve it, and activate it in a water bath at 32°C. After 28 minutes, make an activated enzyme solution and set it aside; mix Hypericum perforatum powder with 10,000 g of water, soak in a water bath at 40°C for 90 minutes, cool to 18°C, adjust the pH to 5.3 with hydrochloric acid, shake well, heat in a water bath to 55°C, add the above activation Enzyme solution, mixed evenly, enzymolysis for 2 hours, suction filtration, discarding the filtrate to obtain the enzymolysis medicinal powder, adding 6 times the weight of the enzymatic hydrolysis medicinal powder to the enzymatic hydrolysis medicinal powder for reflux extraction 3 times, each time for 3 hours, filter, combine the filtrates, concentrate under reduced pressure at 50°C, recover ethanol, and dr...

Embodiment 2

[0015] First dry Hypericum perforatum, crush it through a 20-mesh sieve, and form Hypericum perforatum powder. Weigh 2kg of Hypericum perforatum powder, then weigh 30g of pectinase, add 450g of warm water at 45°C to the pectinase, dissolve it, and activate it in a water bath at 45°C for 20 Minutes into an activated enzyme solution for later use; Hypericum perforatum powder is mixed with 30,000 g of water, soaked in a 70°C water bath for 50 minutes, cooled to 20°C, adjusted to pH 4 with hydrochloric acid, shaken well, heated in a water bath to 47°C, and added with the above-mentioned activated enzyme solution Enzyme solution, mixed evenly, enzymolysis for 5 hours, suction filtration, discarding the filtrate to obtain the enzymolysis medicinal powder, adding 13 times the weight of the enzymolysis medicinal powder to the enzymatic hydrolysis medicinal powder for reflux extraction 2 time, each time for 1.5 hours, filtered, combined filtrates, concentrated under reduced pressure at ...

Embodiment 3

[0017] First dry Hypericum perforatum, crush it through a 20-mesh sieve to form Hypericum perforatum powder, weigh 3kg of Hypericum perforatum powder, then weigh 75g of xylanase, add 1500g of xylanase to warm water at 58°C, dissolve, and put it in a water bath at 58°C Activate for 8 minutes to form an activated enzyme solution for later use; mix Hypericum perforatum powder with 60,000 g of water, soak in a 90°C water bath for 20 minutes, cool to 25°C, adjust the pH to 4.5 with hydrochloric acid, heat in a water bath to 40°C, and add the activated enzyme Solution, mixed evenly, enzymatic hydrolysis for 11 hours, suction filtration, discarding the filtrate to obtain the enzymolyzed medicinal powder, adding 20 times the weight of the enzymatically hydrolyzed medicinal powder to the enzymolyzed medicinal powder and refluxing extraction for 5 times , 5 hours each time, filter, combine the filtrates, concentrate under reduced pressure at 55°C, reclaim ethanol, and dry to obtain Hyper...

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PUM

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Abstract

The invention relates to a biological preparation method of a Hypericum perforatum L extractive, providing a preparation method which can utilize medicinal material resources adequately, has simple process, is suitable for large-scale production and can prepare the Hypericum perforatum L extractive in accordance with or higher than international standards. The method comprises the following steps of: drying the Hypericum perforatum L firstly and smashing into Hypericum perforatum L powder, then weighing enzyme, adding warm water in the enzyme to dissolve the enzyme, activating enzyme solution by water bath, mixing the Hypericum perforatum L powder with water, performing water bath, soaking, cooling, adjusting pH with hydrochloric acid, stirirng uniformly, heating by water bath, adding the actived enzyme solution, mixing uniformly, performing enzymolysis, filtering, abandoning the filter to obtain medicinal material powder after enzymolysis, adding ethanol and fluxing for extraction, filtering, combining the filtrate, condensing under reduced pressure, recycling ethanol, and drying to obtain the Hypericum perforatum L extractive. The invention improves the content of hypericin in the extractive and the abandoned filtrate after enzymolysis takes away a large amount of water-soluble impurities so that the moisture of the extractive is not needed to absorb again. The biological method is safe, pollution free and has simple operation and is easy for industrialized application.

Description

1. Technical field [0001] The invention relates to the field of medicine, in particular to a biological preparation method of Hypericum perforatum extract. 2. Background technology [0002] Hypericin (chemical name: 4,4,5,5,7,7-hexahydroxy-2,2-dimethyl-meso-naphtho) contained in Hypericum Perforatum L. Dianthrone) has excellent biological activities, such as antidepressant, antiviral, anticancer and photodynamic activity, especially for a series of neurological disorders such as depression and sub-health symptoms caused by tension. The medical profession pays attention. The increasing demand for Hypericum perforatum extract in the international market has also stimulated the production of Hypericum perforatum extract in my country in recent years. [0003] However, the content of hypericin in the whole plant of Hypericum perforatum is only a few ten-thousandths. Therefore, how to prepare qualified (the 24th edition of the United States Pharmacopoeia and the import and expo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P25/24A61P31/12A61P35/00A61P25/00
Inventor 杨云刘富岗
Owner HENAN UNIV OF CHINESE MEDICINE
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