Preparation method of protein blot membrane regeneration liquor

Abstract

The invention relates to a preparation method of protein blot membrane regeneration liquor, belonging to the technical field of biological detection. The preparation method comprises the following steps of: respectively weighing glycine and sodium dodecyl sulfate and preparing into an aqueous solution by ensuring that the molar concentration of the glycine is 20 millimole / liter, and the mass percent concentration of the sodium dodecyl sulfate is 1 percent; and regulating the pH of the aqueous solution by using hydrochloric acid. The protein blot membrane regeneration liquor prepared by using the method has strong efficacy and can efficiently and specifically dissociate the combination of antigens and antibodies but has moderate action on protein combined onto the membrane. The regeneration liquor does not contain beta-mercaptoethanol, has no poison and smell, no toxic hazard to experiment operators, simple use method, quick operation and convenient storage and can be stored at room temperature.

Description

technical field [0001] The invention relates to a method for preparing protein imprinted membrane regeneration solution, belonging to the technical field of biological detection. Background technique [0002] Western blotting is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. Its basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies, and analyze the coloring position and Depth of Staining Obtain information on how specific proteins are expressed in the cells or tissues being analyzed. The specific test method is to transfer the protein sample separated by polyacrylamide gel electrophoresis to a solid phase support (such as nitrocellulose membrane or polyvinylidene fluoride membrane). Keep the types of peptides separated by electrophoresis and their biological activities unchanged. Use the protein or polypeptide on the solid phase carrier as the antigen, react...

AI Technical Summary

Problems solved by technology

[0004] The protein imprinting membrane regeneration method introduced in traditional molecular cloning is too strong for antigen elution, which often leads to the weakening of protein signals transferred to solid phase supports such as nitrocellulose membrane or polyvinylidene fluoride membrane)

And it contains mercaptoethanol, a highly irritating chemical substance, which will cause harm to operators if it is not handled carefully.

Moreover, the traditional formula requires a long time to operate