Neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide, and genes and application thereof

A technology of galactosidase and aga-s27, which is applied in the field of genetic engineering and can solve problems such as inability to hydrolyze various substrates
CN101838618AInactive Publication Date: 2010-09-22JIANGSU YINONG BIOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGSU YINONG BIOLOGY CO LTD
Publication Date
2010-09-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of genetic engineering and particularly provides neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide, and genes and application thereof. The neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide can be obtained from a bacterial strain, i.e., novel streptomyces sp.S27 (CGMCC No.3146) screened by the invention, and the genes thereof and recombinant vector containing the genes can be further obtained, wherein the amino acid sequences of alpha-galactosidase Aga-S27 are shown in SEQ ID No.1. The alpha-galactosidase Aga-S27 has the advantages of appropriate operating temperature and pH value, higher protease resistance and higher ability to hydrolyze various substrates. Therefore, the alpha-galactosidase Aga-S27 is applicable as feed or food additives in the industries of feed and food.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a neutral alpha-galactosidase Aga-S27 with high alpha-galactoside oligosaccharide degrading ability and its gene, a recombinant vector containing the gene and application thereof. Background technique

[0002] α-galactosidase (α-galactosidase, EC3.2.1.22) is melibiase, which belongs to the class of exoglycosidases and can specifically catalyze the hydrolysis of α-galactosidic bonds at the non-reducing ends of sugar chains. Not only can it hydrolyze oligosaccharides containing α-galactosidic bonds, but it can also catalyze polysaccharides containing such bonds. Raffinose, stachyose, and verbascose are oligosaccharides widely present in legumes. Under the action of α-galactosidase, these oligosaccharides can be decomposed into D-galactose and other corresponding monosaccharides. sugars and oligosaccharides.

[0003] Soybean meal is a very good plant-based...

Claims

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