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Isolated cell mass

A cell group, cell technology, applied in the field of cell groups

Inactive Publication Date: 2010-09-29
KYOWA HAKKO KIRIN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0049] When in the transplantation of various cells such as hematopoietic stem cells, hematopoietic progenitor cells, etc., excellent migration and engraftment of the transplanted cells to the bone marrow and damaged tissues can be obtained, it can be expected to obtain a therapeutic effect using fewer cells, and can It is expected not only to improve the therapeutic effect, but also to solve the problems mentioned above

Method used

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  • Isolated cell mass
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0153] Preparation of Antibody, Selectin-IgG Fusion Protein and α1,3-Fucosyltransferase

[0154] Fluorescein isothiocyanate (FITC)-labeled anti-human Lewis X antibody (HI 98), FITC-labeled anti-mouse IgM antibody (II / 41), IgM isotype control antibody (11E10), Phycoerythrin Protein (PE)-labeled anti-human CD38 antibody (HIT 2), PE-labeled anti-mouse CD45 antibody (30-F11), PE-labeled anti-mouse TER-119 antibody (TER-119) and Allosa Procyanin (APC)-labeled human CD34 antibody (581 ) was purchased from BectonDickinson Pharmingen. FITC-labeled and APC-labeled human CD45 antibodies (HI30) were purchased from eBioscience. FITC-labeled anti-human IgG antibody was purchased from DAKOCytomation.

[0155] As an anti-human sialyl Lewis X sugar chain antibody (KM 93), an antibody collected from a hybridoma culture supernatant was used [Hanai et al., Cancer Research, 46 , 4438-4443 (1986)].

[0156] A fusion protein of human E-selectin and human IgG constant region (Fc) (hereinafter ...

Embodiment 2

[0206] Migration of Mesenchymal Stem Cells to the Liver in a Hepatitis Model

[0207] 1. Lewis X sugar chains and sialyl Lewis X sugar chains in human mesenchymal stem cells expression analysis of

[0208] Human mesenchymal stem cells (hereinafter referred to as hMSCs) were purchased from CAMBREX. Place hMSCs in CO 2 In an incubator, use MSCGM medium (manufactured by CAMBREX) at 37°C and 5% CO 2 cultured under conditions. After washing with phosphate-buffered saline (hereinafter referred to as PBS(-)), the cells were detached by adding TrypLE Select (manufactured by Invitrogen) and treated at 37°C for 2 minutes, and then by adding PBS containing 5% FBS (-) solution terminates the reaction. Cells were recovered by centrifugation at 1,000 rpm (rev / min) for 5 minutes, washed with PBS(-), and then 1-2×10 6 Each cell is suspended in 80-100 μ l of Hanks' balanced salt solution (HBSS) reaction solution [25mmol / l HEPES (manufactured by Gibco), 10mmol / lMnCl 2 (manufactured ...

Embodiment 3

[0222] Migration of Mesenchymal Stem Cells to the Lung in a Pneumonia Model

[0223] 1. Preparation of mouse carbon tetrachloride lung disease model

[0224] Five-week-old male BALB / c-nu / nu Slc mice (Japan SLC) were adaptively bred for 1 week. By diluting carbon tetrachloride (manufactured by Wako Pure Chemical Industries) 10 times with olive oil (manufactured by Wako Pure Chemical Industries), small amounts of carbon tetrachloride to BALB / c-nu / nu Slc were carried out at a dose of 1 ml / kg. Intraperitoneal administration of mice induced acute lung disease [Mizuguchi et al., BioFactors, 26 , 81-92 (2006)].

[0225] 2. hMSC transplantation into mouse carbon tetrachloride lung disease model and analysis

[0226] By the same method as in Example 2, FT 7 enzyme-treated hMSCs or negative control hMSCs were suspended in PBS(-) and adjusted to 2.5×10 6 cells / ml. 24 hours after administration of carbon tetrachloride, with 5 × 10 5 FT 7 enzyme-treated hMSCs or control hMSCs w...

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Abstract

A cell is demanded which can exhibit an excellent ability of penetrating into a tissue and an excellent ability of adhering to a tissue in hematopoietic stem cell transplantation, repair of an injured tissue utilizing stem cell transplantation, or the like. Thus, disclosed are: a mass of cells having an excellent ability of penetrating into a tissue and an excellent ability of adhering to a tissue; a pharmaceutical agent comprising the cells; and a method for the transplantation of the cells. The cells are useful for hematopoietic stem cell transplantation, repair of an injured tissue utilizing stem cell transplantation, or the like.

Description

technical field [0001] The present invention relates to a cell group with excellent tissue migration ability and engraftment ability, a pharmaceutical preparation containing the cell, and a transplantation method of the cell. Background technique [0002] Since the primary purpose of performing cell therapy is to treat damaged tissue, transplantation using hematopoietic stem cells or hematopoietic progenitor cells has been performed. In addition, it has been reported that adult tissue stem cells and progenitor cells exist in various tissues and organs in living bodies, and treatments using them have also been attempted. [0003] Adult tissue stem cells are stem cells that exist in various living tissues. These cells are thought to possess self-renewal and differentiation capabilities that help maintain tissue homeostasis. Stem cells proliferate and are maintained in tissues. Meanwhile, stem cells have only a very limited ability to proliferate, so that stem cells differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A61L27/00C12N15/09A61K35/12C12N5/0775C12N5/0789C12N5/0797
CPCA61K2035/124C12N2501/70C12N5/0006C12N5/0663A61L27/3804C12N5/0647C12N5/0623A61P35/00A61P35/02A61P37/02A61P37/06
Inventor 宫地宏昌一角昌广佐藤秀尚
Owner KYOWA HAKKO KIRIN CO LTD
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