Preparation method of agrocybe aegerita fermentation whitening lotion
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A kind of fermentation liquid and tea tree mushroom technology, which is applied in the application field of bioengineering technology
Inactive Publication Date: 2010-10-13
JIANGNAN UNIV
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The fermented liquid obtained through submerged fermentation plus other components to obtain the fermented liquid with ...
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example 1
[0009] 1. Fermentation Broth Preparation
[0010] The starting strain is commercially available tea tree mushroom (Agrocybe aegirit);
[0011] (1) Tissue separation method
[0012] Slant medium: PDA medium, containing 1.5% to 2.0% agar, with natural pH;
[0013] Tissue separation method: After surface disinfection of the fruiting body with alcohol, put it on the ultra-clean workbench, use a sterilized blade and tweezers to cut out the tissue block at the junction of the stipe and the cap and place it on the slant medium, the pH is natural, Place it in dark culture at 25°C, after 5 days mycelia will start to germinate, check once every 2 days to remove contamination, and select the test tube with fast mycelium growth and strong growth as the pure mother species;
[0014] (2) Shake flask seed culture
[0015] Shake flask seed medium: PDA1L plus 1g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 2g of beef extract, 1g of peptone, pH6.0;
[0016] Cultivation meth...
example 2
[0028] Amino acid composition and monosaccharide composition were measured on tea tree mushroom fermented liquid samples. The measurement results showed that among the contained amino acids, glutamic acid accounted for more than 18%, aspartic acid accounted for more than 12%, glycine accounted for more than 8%, and lysine It accounts for more than 7%, and alanine accounts for more than 6%. The monosaccharide contained is mainly glucose, and the polysaccharide content measured by the phenol-sulfuric acid method is 76.3%. The amino acid composition and content of tea tree mushroom fermentation broth are shown in Table 1.
[0029] Amino acid composition and content of table 1 tea tree mushroom fermentation liquid
[0030]
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Abstract
The invention belongs to the field of bioengineering technology application, and in particular relates to a preparation method of agrocybe aegerita fermentation whitening lotion. The tea tree mushroom fermentation lotion is yellow liquid and is prepared by concentrating, deproteinizing and dialyzing liquid obtained from the submerged fermentation of the agrocybe aegerita. In the fermentation liquid, amino acid comprises the components of more than 18 percent of glutamic acid, more than 12 percent of aspartic acid, more than 8 percent of glycine, more than 7 percent of lysine and more than 6 percent of alanine, and besides, VC kojic acid ester and hyaluronic acid are also contained. The invention has whitening activity and can be applied to the preparation of cosmetics with the whitening effect.
Description
technical field [0001] The invention relates to a preparation method of tea tree mushroom fermented whitening liquid. It belongs to the application field of bioengineering technology. Background technique [0002] Tea Tree Mushroom belongs to Basidiomycetes, Copromataceae, Field Mushroom, also known as Tea Mushroom, Camellia oleifera, and God Mushroom. It is a new variety of edible fungi developed in recent years. Tea Tree Mushroom is rich in nutrients, high in protein, contains essential amino acids, and is rich in B vitamins and mineral elements such as potassium, sodium, calcium, magnesium, iron, and zinc. Traditional Chinese medicine believes that the mushroom is flat, sweet and warm, non-toxic, and has high medicinal value. It has the effects of clearing away heat, calming the liver, improving eyesight, and strengthening the spleen. It is a green food integrating nutrition, health care and physiotherapy. The fermented liquid obtained by submerged fermentation plus o...
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