Aeromonas hydrophila micro-capsular oral vaccine

A technology of Aeromonas hydrophila and oral vaccine, which is applied in the field of biopharmaceuticals, can solve problems such as poor immune effect, and achieve the effects of safe reagents, convenient operation, convenient storage and transportation

Inactive Publication Date: 2010-10-20
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

And the microcapsule antigen delivery system was applied to Aeromonas hydrophila, and the microcapsule oral vaccine o

Method used

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  • Aeromonas hydrophila micro-capsular oral vaccine
  • Aeromonas hydrophila micro-capsular oral vaccine
  • Aeromonas hydrophila micro-capsular oral vaccine

Examples

Experimental program
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Effect test

preparation example Construction

[0041] 1.2 Preparation of inactivated vaccine

[0042] Ah J for rejuvenating infected crucian carp 1 Inoculate in a 500mL flask containing 250mL LB broth, and incubate at 28°C with shaking at 150rpm for 24h. After staining and microscopic examination, formalin with a final concentration of 0.4% was added and reacted at 28°C for 3h, then at 4°C for 24h. Then take a small amount of inactivated bacterial liquid to inoculate LB broth, and culture at 28°C with shaking for 24 hours to check whether the inactivation is complete. After the completely inactivated bacterial solution was centrifuged at 8000rpm for 10min, the bacteria were washed three times with PBS solution of pH 7.2. Adjust the final concentration of bacteria to 1.0 × 10 10 cfu / mL, stored at 4°C for later use.

[0043] 1.3 Preparation of Aeromonas hydrophila sodium alginate-chitosan microcapsules (Ah-ACMS) oral vaccine

[0044] According to the orthogonal design scheme in Table 1, 9 levels were set up to carry out...

Embodiment 3A

[0079] The immunoprotective effect of embodiment 3Ah-ACMS oral vaccine

[0080] The gibel crucian carp of 120-150 g is used as a model animal and raised in a 300L glass tank, and the gibel carp is fed with aerated tap water, and the oxygenation pump is used to continuously aerate the gibel carp. Regularly feed commercial pellet feed, change water and clean up solid metabolites. The crucian carp was acclimatized for a week, and after eating normally, the experiment began. The immune effect of the Ah-ACMS oral vaccine of the present invention on crucian carp was verified according to the following scheme.

[0081] 3.1 Immunization procedure

[0082] The test fish were divided into six groups, 40 in each group, fasted for 24 hours before immunization, and immunized according to the scheme in Table 3. The oral control group was fed feed granules containing vaccine at 8:00, 12:00, and 16:00 according to 1% of the fish body weight each time; the Ah oral and injection control grou...

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Abstract

The invention relates to an aeromonas hydrophila micro-capsular oral vaccine, which belongs to the technical field of biological pharmacy. The aeromonas hydrophila micro-capsular oral vaccine is prepared by using sodium alga acid and chitosan as wall materials, using the inactivated bacteria of aeromonas hydrophila as a core material and by using improved atomization and ion crosslinking technology. When the aeromonas hydrophila sodium alga acid-chitosan micro-capsular oral vaccine is used for immunizing a mouse and crucian, the phagocytosis activities of macrophages, the conversion efficiency of the splenic lymphocytes and the expression amount of cell factors such as IFN-gamma and Il-4 of the mouse and the crucian can be improved obviously. The relative protection rate of the vaccine for the crucian is 39.3 percent. A microcapsule is stable in a simulated gastrointestinal environment and shows high burst release and slow release; and the bacterial antigenicity of the microcapsule is well maintained and the vaccine has high safety and high storage stability.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals. Specifically, the present invention relates to an oral vaccine against Aeromonas hydrophila. More specifically, the present invention relates to a microcapsule antigen delivery system and an oral microcapsule vaccine of Aeromonas hydrophila developed based on it, which can be used to prevent hemorrhagic sepsis caused by Aeromonas hydrophila. The microcapsule antigen delivery system can also be applied to the development of oral vaccines for other fish pathogens. Background technique [0002] Aeromonas hydrophila (Aeromonas hydrophila, Ah) can cause hemorrhagic septicemia outbreaks in fish, amphibians and reptiles, leading to skin bleeding (hemorrhagic septicemia) and mass death in freshwater fish. There was a huge economic loss. The application of traditional therapies such as antibiotics can easily induce a series of problems such as bacterial resistance, drug residues, and environmental eco...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K39/106A61K47/36A61P31/04A61P7/04
Inventor 姚火春陆承平曲向阳
Owner NANJING AGRICULTURAL UNIVERSITY
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