High-sensitivity biological sensor and preparation method thereof

A biosensor and high-sensitivity technology, applied in the field of biosensors, can solve the problems of inability to quantitatively detect the detection process, low sensor detection sensitivity, and long detection time, and achieve the effects of low detectable concentration limit, low price, and rapid response

Inactive Publication Date: 2010-10-20
DONGHUA UNIV
View PDF2 Cites 31 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a high-sensitivity biosensor and its preparation method to solve the problems of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] (1) At room temperature, 2g of polystyrene (polymerization degree of 1600) solid was dissolved in 18g of N,N-dimethylformamide with a mass ratio of 3 / 1 with a magnetic stirrer at a speed of 50rpm / min. In the mixed solvent of tetrahydrofuran, a polystyrene solution with a mass fraction of 10% is obtained;

[0025] (2) Under the conditions of room temperature and humidity of 40%, input a 10% mass fraction of polystyrene solution into the spinneret at a flow rate of 4mL / h, and connect the spinneret to a 20kV power supply for electrostatic spinning Prepare fiber

[0026] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 15cm;

[0027] (4) The fiber deposited on the quartz crystal microbalance electrode was sprayed with gold film with a vacuum metal sputtering apparatus, and the spraying time was 1 min;

[0028] (5) Soak the electrode of the quartz crystal microbalance ...

Example Embodiment

[0031] Example 2

[0032] (1) At room temperature, 3g polyaniline (polymerization degree 300) was dissolved in 17g N,N-dimethylformamide with a magnetic stirrer at 250 rpm / min to obtain a 15% polyaniline mass fraction. Aniline solution

[0033] (2) Under the condition of room temperature and humidity of 50%, input the polyaniline solution with a mass fraction of 15% into the spinneret at a flow rate of 2mL / h, and connect the spinneret to an 18kV power supply for electrospinning preparation fiber;

[0034] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 10cm;

[0035] (4) Use a vacuum metal sputtering apparatus to spray silver film on the fiber deposited on the quartz crystal microbalance electrode, and the spraying time is 2min;

[0036] (5) Soak the electrode of the quartz crystal microbalance sprayed with fiber and silver film in dithiothreitol for 20 hours;

[0037] (6) After taking it ...

Example Embodiment

[0039] Example 3

[0040] (1) At room temperature, 6g of polyethyleneimine (polymerization degree of 500) was dissolved in 14g of methylene chloride with a magnetic stirrer at 500rpm / min to obtain a polyethyleneimine with a mass fraction of 30%. Base imine solution;

[0041] (2) Under the condition of room temperature and humidity of 60%, input the polyethyleneimine solution with a mass fraction of 30% into the spinneret at a flow rate of 6mL / h, and at the same time connect the spinneret to a 60kV power supply. Electrospinning to prepare fibers;

[0042] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 30cm;

[0043] (4) Use a vacuum metal sputtering instrument to spray copper film on the fiber deposited on the quartz crystal microbalance electrode, and the spray time is 10 minutes;

[0044] (5) Put the electrode of the quartz crystal microbalance sprayed with fiber and gold film into glyc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a high-sensitivity biological sensor and a preparation method thereof. The high-sensitivity biological sensor comprises a quartz crystal micro-balance, and is characterized in that polymer fibers are deposited on an electrode of the quartz crystal micro-balance, a metal film covers the polymer fibers, a self-assembling material covers the metal film, and the self-assemblingmaterial is connected with a biological sensing material. The preparation method is characterized by comprising the following steps that: dissolving a spinning polymer raw material in solvent to obtain an electro-spinning raw material; electro-spinning the electro-spinning raw material; depositing the spun fibers on an electrode of the quartz crystal micro-balance; spraying a metal film on the fibers; soaking the electrode of the quartz crystal micro-balance into a self-assembling material solution; taking out the electrode of the quartz crystal micro-balance to be dried and to be connected with a biological sensing material so as to obtain the high-sensitivity biological sensor. The high-sensitivity biological sensor has the advantages that the sensitivity is high, and the quantitative detection can be precisely performed.

Description

technical field [0001] The invention relates to a high-sensitivity biosensor and a preparation method thereof, belonging to the field of biosensors. Background technique [0002] Traditional biosensors are cumbersome to detect biological processes, have low sensitivity, and take a long time to detect, making it difficult to adapt to actual needs. At present, kits are commonly used in the market to detect organisms. Although the shortcomings of the above-mentioned sensor detection methods have been overcome to a certain extent, the kit detection method can only detect qualitatively, not quantitatively, and the detection is not accurate. Some domestic and foreign experts and scholars use quartz crystal microbalance as a research platform to quantitatively detect organisms, and adopt various methods to improve detection sensitivity, such as covering sensitive materials on electrodes in the form of solid films to prepare biosensors, but they still cannot overcome the sensitivity...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/26G01N27/327D06M11/83D06M101/20D06M101/30D06M101/26D06M101/38
Inventor 丁彬孙敏俞建勇孙刚
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products