High-sensitivity biological sensor and preparation method thereof
A biosensor and high-sensitivity technology, applied in the field of biosensors, can solve the problems of inability to quantitatively detect the detection process, low sensor detection sensitivity, and long detection time, and achieve the effects of low detectable concentration limit, low price, and rapid response
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[0023] Example 1
[0024] (1) At room temperature, 2g of polystyrene (polymerization degree of 1600) solid was dissolved in 18g of N,N-dimethylformamide with a mass ratio of 3 / 1 with a magnetic stirrer at a speed of 50rpm / min. In the mixed solvent of tetrahydrofuran, a polystyrene solution with a mass fraction of 10% is obtained;
[0025] (2) Under the conditions of room temperature and humidity of 40%, input a 10% mass fraction of polystyrene solution into the spinneret at a flow rate of 4mL / h, and connect the spinneret to a 20kV power supply for electrostatic spinning Prepare fiber
[0026] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 15cm;
[0027] (4) The fiber deposited on the quartz crystal microbalance electrode was sprayed with gold film with a vacuum metal sputtering apparatus, and the spraying time was 1 min;
[0028] (5) Soak the electrode of the quartz crystal microbalance ...
Example Embodiment
[0031] Example 2
[0032] (1) At room temperature, 3g polyaniline (polymerization degree 300) was dissolved in 17g N,N-dimethylformamide with a magnetic stirrer at 250 rpm / min to obtain a 15% polyaniline mass fraction. Aniline solution
[0033] (2) Under the condition of room temperature and humidity of 50%, input the polyaniline solution with a mass fraction of 15% into the spinneret at a flow rate of 2mL / h, and connect the spinneret to an 18kV power supply for electrospinning preparation fiber;
[0034] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 10cm;
[0035] (4) Use a vacuum metal sputtering apparatus to spray silver film on the fiber deposited on the quartz crystal microbalance electrode, and the spraying time is 2min;
[0036] (5) Soak the electrode of the quartz crystal microbalance sprayed with fiber and silver film in dithiothreitol for 20 hours;
[0037] (6) After taking it ...
Example Embodiment
[0039] Example 3
[0040] (1) At room temperature, 6g of polyethyleneimine (polymerization degree of 500) was dissolved in 14g of methylene chloride with a magnetic stirrer at 500rpm / min to obtain a polyethyleneimine with a mass fraction of 30%. Base imine solution;
[0041] (2) Under the condition of room temperature and humidity of 60%, input the polyethyleneimine solution with a mass fraction of 30% into the spinneret at a flow rate of 6mL / h, and at the same time connect the spinneret to a 60kV power supply. Electrospinning to prepare fibers;
[0042] (3) Deposit the spun fiber on the electrode of the quartz crystal microbalance, the distance between the receiving electrode and the spinneret is 30cm;
[0043] (4) Use a vacuum metal sputtering instrument to spray copper film on the fiber deposited on the quartz crystal microbalance electrode, and the spray time is 10 minutes;
[0044] (5) Put the electrode of the quartz crystal microbalance sprayed with fiber and gold film into glyc...
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