Kit and method for detecting Macrobrachium rosenbergii Nodavirus

Abstract

The invention relates to a kit and a method for detecting Macrobrachium rosenbergii Nodavirus. The kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleic acid denaturation tube filled with TE buffer solution, an amplification test tube filled with amplification reaction liquid and dyes, a negative control tube, a positive control tube, an FTA membrane, quick drying liquid for shortening time of preparing a sample to be detected, namely nucleic acid, and the like. The kit combines the advantages of isothermal amplication and quick dye detection, does not need precious instruments in the detection process, and develops by adopting a dye filling method to improve the detection reliability. The kit has the advantages of low detection cost, convenient use and safety for human bodies and environment, and can replace the conventional related detection methods. The kit can be used for production field in the open field, and has important promotion and application value of enhancing the monitoring of the Macrobrachium rosenbergii Nodavirus and preventing the large-scale outbreak of the Macrobrachium rosenbergii Nodavirus.

Description

technical field [0001] The invention relates to a marine biological pathogen detection technology, in particular to a kit and a detection method for Macrobrachium rosenbergii Nodavirus (MrNV for short). Background technique [0002] Macrobrachium rosenbergii Nodavirus (MrNV) is a non-enveloped RNA virus with a size of about 26-27nm and an icosahedral shape. Its genome consists of two single-stranded RNAs; it is often accompanied by Macrobrachium rosenbergii Nodavirus There is also a satellite virus called Extra smallvirus (XSV for short), which is about 14-16nm in size and whose genome consists of a segment of RNA. The virus mainly infects Macrobrachium rosenbergii, and the infected parts are mainly the striated muscle of the shrimp and the connective tissue of the hepatopancreas. The body trunk of the infected shrimp is milky and opaque, so it is also called white tail disease. , referred to as WTD), muscle white disease (White muscledisease, referred to as WMD), the virus...

AI Technical Summary

Problems solved by technology

The pathological section method cannot directly detect the virus, but can only detect the histopathological signs of the disease, and it is also limited to the laboratory; although the electron microscope technology can directly observe the existence of virus particles, its operation is complicated and costly. The time is long and the accuracy is low; although the detection method of Nodavirus in Macrobrachium rosenbergii is faster than the pathological section method, its sensitivity is low, and the Nodavirus in Macrobrachium rosenbergii virus has not yet caused infection or is very early in the infection. Difficult to detect with antibody method; RT-PCR detection method of Macrobrachium rosenbergii Nodavirus, although overcomes the shortcomings of the previous methods, can achieve relatively fast and accurate detection of Macrobrachium rosenbergii Nodavirus under laboratory conditions , but because conventional RT-PCR detection requires expensive PCR machines, gel electrophoresis and imaging systems, it is difficult to use RT-PCR detection methods for rapid on-site detection, which greatly limits the promotion of RT-PCR detection methods in production application

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