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Compositions of increasing microbial populations on surfaces and their uses

A composition, microbial technology, applied in the direction of stimulating growth, application, biochemical equipment and methods by adding compounds, which can solve the problem of increasing the population density or biological activity of microorganisms on the surface of plants, increasing the population density or biological activity of natural microorganisms or beneficial microorganisms. Activity and other issues to achieve the effect of increasing microbial population, prolonging supply time, and improving biological activity

Inactive Publication Date: 2010-11-10
金达洙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports of the application of SAP on plant surfaces to increase the population density or biological activity of natural or beneficial microorganisms
In addition, there are no reports of using SAP to increase the population density or biological activity of microorganisms on plant surfaces, protect plants from harmful microorganisms, or promote plant growth.

Method used

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  • Compositions of increasing microbial populations on surfaces and their uses
  • Compositions of increasing microbial populations on surfaces and their uses
  • Compositions of increasing microbial populations on surfaces and their uses

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0074] 4. Preparation of Microbial Compositions

[0075]The microbial composition of the present invention is prepared by grinding and mixing various ingredients into a powder having an average particle size equal to or less than 0.1 mm. In the composition according to the invention, the SAP is a starch based SAP. The microbial nutrient is PLNT. Beneficial microorganisms are purchased commercially or isolated, purified and cultured in a laboratory, cultured, and then made into powder by freeze-drying technology. In addition, 5% of surfactant in powder form was used to achieve uniform distribution of the composition on the plant surface. In the present invention, the surfactant is Softanol (CAS Registration No. 68131-40-8) manufactured by Ineos Oxide Ltd., UK. Softanol was absorbed at a weight ratio of 1:1 with white carbon ink supplied by Jungwoo Chemical Co., Korea. In more detail, Softanol is a nonionic surfactant obtained by adding ethylene oxide to a linear secondary a...

Embodiment 1

[0084] Example 1: Dynamics of microbial populations

[0085] In this example, the effect of each composition on the population density of natural microorganisms or added beneficial microorganisms present on the plant surface was monitored. For the purposes of the experiment, natural microorganisms were defined as microorganisms obtained as follows: 100 grams of cucumber leaves from adult cucumber plants were vigorously shaken with 1 liter of water in a 250-ml Erlenmeyer flask, allowed to stand for 30 minutes, and then vigorously After shaking, filter with a sterilized double-layer coarse filter cloth (hereinafter collectively referred to as "leaf elution water"). When cultured on LB agar medium, approximately 2×10 5 Colony forming units / milliliter (cfu / ml) (equivalent to 5.3 log colony forming units / ml) of native microorganisms. Among them, bacteria accounted for 90% of the dominant number in the total population. Each composition in the present invention was added to leaf ...

Embodiment 2

[0094] Example 2: Activity of Compositions for Protecting Plant Seedlings from Phytopathogens

[0095] In this example, the compositions of the present invention were tested against phytopathogens (Magnaporthe grisea, Thanatephorus cucumeris, Phytophthora capsici, Botrytis cinerea) in a growing room. ), Puccinia graminis (Puccinia graminis), powdery mildew (Blumeria graminis (Erysiphe graminis) protective activity (Choi, G.j, Kim, J.-C., Lee, S.-W., Jang, K.S., Kim, J .-S., and Cho, K.Y. 2002. Antifungal activity of several plant extracts against wheat rust. Korean Journal of Insecticidal Science 6(2):87-95. Korean). The main difference is: Thing 200 times of dilution sprays after plant surface treatment plant 3 days, inoculates and inoculates phytopathogens respectively.Plant after the treatment maintains by routine operation, estimates plant protection activity by control value.Control value (Control value, CV) is estimated with following calculation formula : CV (%)={1-(tr...

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PUM

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Abstract

The present invention relates to compositions that can improve biological activity or can increase population density of natural or beneficial microorganisms on surfaces. By proper use of the compositions, the present invention provides uses of protecting plants from plant pathogen, harmful insects or weedy plants or of promoting plant growth. By proper use of the compositions in the present invention, biological activity or population density of natural or beneficial microorganisms can be increased and the improved biological activity or population density of the microorganisms can protect plants from harmful organisms including plant pathogens, harmful insects or weedy plants or can promote plant growth.

Description

technical field [0001] The present invention relates to a composition capable of increasing the biological activity of microorganisms or increasing the density of microorganism populations on a surface. The compositions of the present invention can be used to protect plants from phytopathogens, harmful insects or weed-like plants, or to promote the growth of plants. Background technique [0002] On the surface of plants (including leaves, stems, branches, flowers or fruits), the soil surface of grasslands, and the surfaces of animals (such as cockroaches, snails or ants) (hereinafter collectively referred to as "surfaces"), various natural microorganisms. These microorganisms occur in saprophytic, symbiotic or parasitic relationships. On plant surfaces, parasitic phytopathogens cause plant diseases by increasing their populations under favorable environmental conditions, and harmful insects also expand their populations causing damage to plants. Weed-like plants can also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00
CPCC12N1/20A01N25/10C12N1/38C12N1/14A01N63/20A01N63/38A01N63/23A01N63/25A01N63/28A01N63/30C12N11/00C12N11/10
Inventor 金达洙
Owner 金达洙