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Method for preparing small-caliber artificial blood vessel from fish intestines

An artificial blood vessel, small-caliber technology, applied in medical science, prosthesis, etc., can solve the problems of not being able to meet the length requirements of small-caliber artificial blood vessels, difficulty in matching diameters, etc., and achieve favorable adhesion, rich sources, and easy operation easy-to-use effects

Inactive Publication Date: 2010-11-17
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The distance between the longest two branches generally does not exceed 2 cm, which means that the maximum length of the small-diameter artificial blood vessel prepared from the extracellular matrix derived from mammalian blood vessels is about 2 cm, which is obviously It cannot meet the clinical requirements for the length of small-diameter artificial blood vessels
At the same time, it is also difficult to match the pipe diameter

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of fish intestines:

[0031] Take an adult silver carp (Hypophthalmichthys molitrix), open the abdominal cavity, take out the fish intestine, remove the fascia and fat, remove the contents of the fish intestine, wash it, and cut it into 100 mm length.

[0032] (2) Decellularization of fish intestines:

[0033] Degreasing: Soak the fish intestines in soapy water, stir for 10 minutes every hour, and repeat the operation 12 times;

[0034] Rinsing: Take out the fish intestines, wrap them in gauze, put them under the tap, and rinse them with running water for 8 hours;

[0035] Trypsin treatment: soak the fish intestines in a trypsin solution with a concentration of 2.5g / L for 8 hours;

[0036] Compound enzyme treatment: Soak fish intestines in a solution containing 30U / ml DNase and 0.025mg / ml RNase for 4 hours;

[0037] Rinse: Take out the fish intestines, wrap them in gauze, put them under the tap, and rinse them with running water for 2 hours;

[0038]...

Embodiment 2

[0044] (1) Preparation of fish intestines:

[0045] Take an adult bighead carp (Aristichthys mobilis), open the abdominal cavity, take out the fish intestines, remove the fascia and fat, remove the contents of the fish intestines, wash and cut into 150mm lengths.

[0046] (2) Decellularization of fish intestines (as in Example 1):

[0047] (3) Preparation of small-diameter artificial blood vessels:

[0048] Soak the extracellular matrix derived from fish intestines obtained in step (2) in PLGA chloroform solution with a concentration of 10 g / L for 1 hour, take it out, air-dry it naturally, rinse it with phosphate buffered saline (PBS) 3 times, each time 1 hours, freeze-dried, and sealed.

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PUM

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Abstract

The invention applies an extra-cellular matrix from fish intestines, a brand-new good and cheap material, to research and manufacture of a small-caliber artificial blood vessel for the first time at home and abroad. The fish intestines are rich in source and good and cheap, so the prepared small-caliber artificial blood vessel has low cost. The extra-cellular matrix from the fish intestines is the same as the small-caliber blood vessel in form, so the operation is simple, convenient and feasible. The prepared small-caliber artificial blood vessel does not fracture, leak blood or cause coagulation under the blood pressure of a human body, is favorable for the conglutination of endothelial cells of the blood vessel, and can meet the requirements for small caliber and length of the blood vessel at the same time.

Description

technical field [0001] The invention relates to the technical fields of biological tissue engineering and biomaterials, in particular to a method for preparing a small-caliber artificial blood vessel, specifically a method for preparing a small-caliber artificial blood vessel using fish intestines as a raw material. Background technique [0002] Clinically, there are many vascular lesions that require vascular transplantation for patients, such as heart bypass, replantation of severed limbs, vascular defects caused by open trauma, and loss of local vascular function caused by atherosclerosis. In vascular grafting, a large number of grafts are required. The best source of these grafts is blood vessels from other parts of the patient's body, however, this will lead to varying degrees of damage to the body at the site where the grafts are taken. For this reason, people turn their attention to allogeneic blood vessels, which in addition to facing the problem of limited donors I...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 刘彬张耀光蒲德永王志坚戴丽
Owner SOUTHWEST UNIVERSITY
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