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Separation and purification method for cerebroside and ceramide type compound

A technology for the separation and purification of ceramides, which is applied in the field of functional oil preparation, can solve the problems that are not suitable for large-scale industrial extraction and production of sphingolipids, and the low utilization rate of raw materials and low yield, so as to improve the utilization rate of raw materials, The effect of low production cost and high yield

Active Publication Date: 2010-11-17
广州利众生物科技有限公司
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Problems solved by technology

Application No. 200510068282 of the Chinese patent "Method for preparing cerebroside compounds from flax root" claims to protect the method of extracting, separating and purifying cerebroside substances from flax root, but due to the low utilization rate of raw materials, the yield Low cost, high cost, not suitable for large-scale industrial extraction and production of sphingolipids in food, cosmetics and medical industries

Method used

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  • Separation and purification method for cerebroside and ceramide type compound
  • Separation and purification method for cerebroside and ceramide type compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: (processing route is as figure 1 shown)

[0024] (a) Extraction of crude cerebrosides: Weigh 30.0g of soybean lecithin into a 250mL conical flask, add 30ml of petroleum ether, connect a straight condenser, stir and extract under magnetic stirring for 30min, and the extraction temperature is 50°C After the extraction, cool down; transfer the extract to a centrifuge tube and centrifuge to obtain the lower layer of thick cerebroside solids; the upper layer of petroleum ether solution is concentrated and evaporated to dryness to obtain soybean lecithin after extracting cerebrosides, which can be recycled .

[0025] (b) pretreatment of crude cerebroside: the crude cerebroside solid in the lower layer is extracted once with petroleum ether (the mass volume ratio of crude cerebroside solid and petroleum ether is 1: 1), removes redundant neutral lipid, obtains Brown powdery insoluble matter; the insoluble matter was extracted 3 times with hot ethanol at a temper...

Embodiment 2

[0033] (a) Extraction of crude cerebrosides: Weigh 30.0g of soybean lecithin into a 250mL conical flask, add 60ml of petroleum ether, connect a straight condenser, stir and extract for 60min under magnetic stirring, and the extraction temperature is 30°C After the extraction, cool down; transfer the extract to a centrifuge tube and centrifuge to obtain the lower layer of thick cerebroside solids; the upper layer of petroleum ether solution is concentrated and evaporated to dryness to obtain soybean lecithin after extracting cerebrosides, which can be recycled .

[0034] (b) Pretreatment of crude cerebroside: the crude cerebroside solid in the lower layer is extracted once with normal hexane (the mass volume ratio of crude cerebroside solid and normal hexane is 1: 2), removes unnecessary neutral lipid, obtains Brown powdery insoluble matter; the insoluble matter was extracted 3 times with hot ethanol (the mass volume ratio of insoluble matter and hot ethanol was 1:3) at a tempe...

Embodiment 3

[0042] (a) Extraction of crude cerebrosides: Weigh 30.0g of soybean lecithin into a 250mL conical flask, add 90ml of petroleum ether, connect a straight condenser, stir and extract under magnetic stirring for 45min, and the extraction temperature is 40°C After the extraction, cool down; transfer the extract to a centrifuge tube and centrifuge to obtain the lower layer of thick cerebroside solids; the upper layer of petroleum ether solution is concentrated and evaporated to dryness to obtain soybean lecithin after extracting cerebrosides, which can be recycled .

[0043] (b) Pretreatment of crude cerebroside: the crude cerebroside solid in the lower layer is extracted once with industrial hexane (mass volume ratio of crude cerebroside solid and industrial hexane is 1: 3) to remove excess neutral lipid , to obtain a brown powdery insoluble matter; the insoluble matter was then extracted 3 times with hot ethanol (the mass volume ratio of the insoluble matter and hot ethanol was 1...

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Abstract

The invention discloses a separation and purification method for cerebroside and a ceramide type compound. The method comprises the following steps of: extracting soya bean lecithin serving as a raw material with petroleum ether with stirring and centrifuging the mixture; extracting lower-layer rough cerebroside solid with a low-polarity organic solvent; extracting the extracted insoluble substance with hot ethanol; combining the extract and concentrating and evaporating the combined extract to dryness to obtain purified rough cerebroside; separating the rough cerebroside by silica gel columnchromatography to obtain a dry substance I; and separating the dry substance by secondary silica gel column chromatography to obtain pure cerebroside and pure ceramide. The separation and purification method has the advantages of high raw material utilization ratio, simple process, low production cost, high purity of the obtained product, high yield and good industrial prospect.

Description

technical field [0001] The invention belongs to the field of functional oil preparation, and in particular relates to a method for separating and purifying cerebrosides and ceramide compounds. Background technique [0002] Cerebrosides were originally extracted from brain tissue, so they are also called cerebrosides. The cerebrosides found in soybeans are mainly glucoside sphingosine, which contains a glucose molecule, which is composed of sphingosine, fatty acid and D-galactose. The OH group on C-1 combines with the β-OH group on D-galactose to form a β-glucoside. According to whether there is a hydroxyl group in the fatty acid carbon chain, it is divided into non-hydroxy fatty acid (Nonhydroxy fatty acid, NHFA) and hydroxy fatty acid (HFA). Sphingosine has three structures, sphingosine containing unsaturated double bonds is called sphingosine (Sphingosine), saturated sphingosine is called dihydrosphinganine (Sphinganine), and sphingosine containing 4 hydroxyl groups Ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/10C07H1/08C07C233/18C07C231/24C07C215/24C07C213/10
Inventor 汪勇胡长鹰韩雪
Owner 广州利众生物科技有限公司
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