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Method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone

A technology of sperm maturation and streak star, applied in fish farming, application, climate change adaptation, etc., to achieve the effect of improving sperm motility, rapid motility rate, and decreasing semen viscosity

Inactive Publication Date: 2010-11-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

At present, for the slow-release hormone induction of broodstock, muscle implantation and injection are used at home and abroad, and there are no reports of other induction methods.

Method used

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Experimental program
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Embodiment

[0017] At present, there are no reports on the annual testicular cycle development, sperm characteristics and sperm quality of the spotted star flounder male. With the rise of the spotted star flounder aquaculture industry, the seed production industry has more and more needs for the technology of stably obtaining fertilized eggs. more urgent.

[0018] This research was carried out in Qingdao Zhonghai Fisheries Co., Ltd. from March to April 2009. The specific implementation process is as follows:

[0019] 1). Selection of broodstock: Select 8 male flounder (full length 25-33cm, body weight 540-680g) that are in the ejaculation period under the control of artificial temperature and light and can collect milky white semen. The broodstock is MS222 (150mg / L) After anesthesia, the semen was collected by artificial extrusion. Microscopic examination found that the semen had high viscosity, the motility rate of sperm was lower than 45%, the rapid motility rate of sperm was lower tha...

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Abstract

The invention relates to a method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone. Sexually mature parent fish is selected, and salmon gonadotropin releasing hormone (sGnRH) is implanted into the back muscle (35 mu g / kg) to promote the sperm to finally become mature and enhance the sperm activity to more than 85-90%, thereby increasing the fertility rate by 4-7 times. The method has the advantages of low reproductive menace for parent fish, high survival rate and sex gland maturation rate of parent fish, and favorable spawning effect, and can obtain massive high quality oosperms. The method is a complete control technique. When the sperm quality starts to degrade, implantation is carried out on the parent fish for the second time, and the repeating effect is good. The method can ensure the large-scale artificial propagation of fries to be smooth.

Description

Technical field: [0001] The invention belongs to the reproduction control technology of spotted star flounder, which is a method for promoting the final maturation of the sperm of the spotted star flounder male broodstock by using exogenous hormones and improving the vitality of the sperm. Background technique: [0002] Striped star flounder (Verasper moseri) is a large cold-water benthic flounder, mainly distributed in the Pacific coast north of Ibaraki Prefecture in Japan and the coast of the Sea of ​​Japan north of Wakasa Bay. It was also distributed in the Yellow Sea and Bohai Sea in my country before, and now it Haven't seen a catch for many years. In the natural sea area, star flounder mostly lives in coastal waters or bays. The living water depth is generally 10-30m, the living water temperature is 1-20°C, the suitable growth water temperature is 13-20°C, and the suitable growth salinity is 25-33. Under the best breeding conditions, after one year of cultivation, the w...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐永江柳学周孙中之刘新富陈超王妍妍兰功钢
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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