Method for extracting epigallocatechin gallic acid ester from oolong tea by chromatography and membrane technology

A technology of epigallocatechin and gallate, which is applied in the field of separation and purification of epigallocatechin gallate, can solve the problems of toxic organic solvent residue, product difficulty, metal residue, etc., and achieve a scientific and reasonable process design process, Ease of industrialization and low production cost

Inactive Publication Date: 2010-12-22
陈森全
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Because the main active ingredient in tealeaves is tea polyphenols, EGCG is mostly extracted from tealeaves at present. For this, the patent document with publication number CN101381359A of the Patent Office discloses "a kind of extracting high-purity epigallocin from green tea The method of theanine gallate ", by combining liquid phase extraction and flashing silica gel column chromatography, epigallocatechin gallate monomer is prepared, although this method can produce high-purity EGCG monomer, but On the one hand, due to problems such as complex operation, large investment, low yield, large loss, toxic organic solvent (such as chloroform) residue, and metal residue, it is difficult for the product to be accepted by industries such as health care products and medicine; on the other hand, studies have shown that, The effective ingredients tea polyphenols and catechin monomer epigallocatechin gallate (EGCG) in the tea leaves of oolong tea are nearly ten percentage points higher than those of green tea. Therefore, extracting EGCG from green tea is not more effective than oolong tea ideal resource advantage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Extraction, extracting the oolong tea leaves with water to obtain tea liquid.

[0016] 2) Membrane filtration Membrane concentration, at room temperature, use an organic polymer roll-type membrane to remove impurities and color, and then continuously extract and remove tea polysaccharides to obtain a relatively pure tea polyphenol sample solution (TP≥98%, EGCG≥50%).

[0017] 3) Chromatographic separation: 100ml of tea polyphenol sample solution, the tea polyphenol component is about 10g, and the tea polyphenol solution is pumped into the resin adsorption column at normal temperature and pressure, the volume of the resin column is 250cm 3 , that is (1BV=250ml), the adsorption flow rate is 1~2BV / h, select polar macroporous resin for use: the average particle size is about 50 mesh, its effluent is caffeine and pigment, the color changes from dark to light, and the adsorption is complete; use 2BV Wash with water at a flow rate of 1BV / h until the effluent is colorless; de...

Embodiment 2

[0022] 1) Extraction, extracting the oolong tea leaves with water to obtain tea liquid.

[0023] 2) Membrane filtration Membrane concentration, at room temperature, use an organic polymer roll-type membrane to remove impurities and color, and then continuously extract and remove tea polysaccharides to obtain a relatively pure tea polyphenol sample solution (TP≥98%, EGCG≥50%).

[0024] 3) Chromatographic separation: 120ml of tea polyphenol sample solution, the tea polyphenol component is about 12g, and the tea polyphenol solution is pumped into the resin adsorption column at normal temperature and pressure, the volume of the resin column is 250cm 3 , that is (1BV=250ml), the adsorption flow rate is 1~2BV / h, select polar macroporous resin for use: the average particle size is about 50 mesh, its effluent is caffeine and pigment, the color changes from dark to light, and the adsorption is complete; use 2BV Wash with water at a flow rate of 1BV / h until the effluent is colorless; de...

Embodiment 3

[0029] 1) Extraction, extracting the oolong tea leaves with water to obtain tea liquid.

[0030] 2) Membrane filtration Membrane concentration, at room temperature, use an organic polymer roll-type membrane to remove impurities and color, and then continuously extract and remove tea polysaccharides to obtain a relatively pure tea polyphenol sample solution (TP≥98%, EGCG≥50%).

[0031] 3) Chromatographic separation: 200ml of tea polyphenol sample solution, the tea polyphenol component is about 20g, and the tea polyphenol solution is pumped into the resin adsorption column at normal temperature and pressure, and the volume of the resin column is 250cm 3 , that is (1BV=250ml), the adsorption flow rate is 1~2BV / h, select polar macroporous resin for use: the average particle size is about 50 mesh, its effluent is caffeine and pigment, the color changes from dark to light, and the adsorption is complete; use 2BV Wash with water at a flow rate of 1BV / h until the effluent is colorless...

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Abstract

The invention relates to a method for extracting epigallocatechin gallic acid ester from oolong tea by chromatography and membrane technology. The method comprises the following steps of: (1) extracting tea liquid from oolong tea leaves with water; (2) carrying out membrane filtration and membrane concentration for removing impurities and decoloring to obtain purer tea polyphenol liquid; (3) carrying out chromatographic separation for separating catechin solution from the polyphenol liquid; (4) separating by using a polymeric membrane at room temperature to obtain EGCG solution; (5) concentrating the EGCG solution in vacuum and crystallizing to obtain EGCG crystal; and (6) freezing and drying the EGCG crystal to obtain a white solid EGCG product. The method has the advantages of low production cost, high product yield, high product purity of 98%, no residues and the like and is convenient for industrialized promotion.

Description

technical field [0001] The invention relates to a method for extracting epigallocatechin gallate (hereinafter referred to as EGCG) from oolong tea, specifically a method for separating and purifying epigallocatechin gallate from oolong tea by using chromatography and membrane separation methods ester method. Background technique [0002] Tea polyphenol is a natural polyphenol active substance extracted from tea leaves. It is the main component and the most effective component EGCG. Antibacterial, anti-aging and other health care and pharmacological effects. At present, EGCG monomer has been listed as a new anticancer drug that can replace paclitaxel. Because the main active ingredient in tealeaves is tea polyphenols, EGCG is mostly extracted from tealeaves at present. For this, the patent document with publication number CN101381359A of the Patent Office discloses "a kind of extracting high-purity epigallocin from green tea The method of theanine gallate ", by combining l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 陈森全冯文辉陈燕珊陈伟强林立武丁明浩
Owner 陈森全
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