Thick oil-degrading bacteria and application thereof
A technology for degrading bacteria and heavy oil, applied in the field of biological treatment of environmental pollutants, to achieve high-efficiency heavy oil removal rate and good environmental adaptability
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[0022] Example 1
[0023] Screening and isolation of strain CCTCC M 2010172 and its heavy oil degradation performance
[0024] The mud-water mixture contaminated by petroleum hydrocarbons was collected at the Guangzhou Petrochemical General Plant, and an inorganic salt liquid culture medium system with heavy oil as the sole carbon source was used to domesticate heavy oil degrading bacteria. Inorganic salt liquid medium: KH 2 PO 4 0.5g, K 2 HPO 4 ·3H 2 O 1g, NaCl 2g, MgSO 4 Aqueous solution (22.5g / L) 3.0mL, CaCl 2 Aqueous solution (36.4g / L) 1.0ml, FeCl 3 1.0ml aqueous solution (0.25g / L), 1.0ml trace element solution, 1L distilled water. Among them, trace element solution composition: MnSO 4 ·H 2 O 39.9mg / L, ZnSO 4 ·H 2 O42.8mg / L, (NH 4 ) 6 Mo 7 O 24 ·4H 2 O 34.7mg / L. The initial concentration of heavy oil is 1g / L. After several generations of repeated domestication and streaking culture in solid medium (NR), multiple pure bacteria were obtained. The pure bacteria were spread on th...
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[0028] Example 2
[0029] The influence of temperature on the degradation of heavy oil
[0030] The heavy oil inorganic salt liquid medium that had been inoculated with GS02 was placed in a constant temperature shaker at 25, 28, 30, 35, and 40°C for 6 days, and the effect of temperature on the degradation of heavy oil was measured.
[0031] Table 1
[0032]
[0033] Temperature (℃) Degradation rate (%)
[0034]
[0035] 25 34.5
[0036] 28 38.7
[0037] 30 45.7
[0038] 35 39.5
[0039] 40 27.4
[0040]
[0041] It can be seen from Table 1 that the degrading bacteria GS02 has a certain removal effect on heavy oil in the range of 25-35℃, and the degradation effect is best at 30℃, which is 45.7%. The degradation effect does not change significantly in a wide temperature range of 25-35°C. When the temperature reaches 40°C, the degradation rate is reduced. This is because the protein in the bacterial cell is ea...
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[0043] Example 3
[0044] The effect of pH on the degradation of heavy oil
[0045] Adjust the pH value of the inorganic salt liquid culture medium to 6.5, 7.0, 7.2, 7.5 and 8.0 respectively, culture for 6 days under the condition of the initial concentration of heavy oil of 1g / L, and determine the influence of pH value on the degradation of heavy oil.
[0046] Table 2
[0047]
[0048] pH degradation rate (%)
[0049]
[0050] 6.5 31.5
[0051] 7.0 45.7
[0052] 7.2 47.6
[0053] 7.5 38.3
[0054] 8.0 34.9
[0055]
[0056] As can be seen in Table 2, when the pH value is 7.2, the degrading bacteria GS02 has the strongest degradation effect on heavy oil, and the degradation rate can reach 47.6% in 6 days; when the pH value is 6.5, the degradation rate is lower, 31.5%. The pH value mainly affects the activity of bacterial enzymes, the permeability and stability of the cell plasma membrane, thereby affecting the degrad...
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