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Process for extracting active collagen peptide from salmon trout waste

A technology of salmon and trout, waste, applied in the field of extracting active collagen peptides, can solve the problems of wastes that cannot be processed, and achieve the effects of reducing the amount of decolorization, saving raw materials, and reducing losses

Inactive Publication Date: 2010-12-29
LANZHOU CITY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Gansu is rich in cold spring water resources. The existing salmon and trout farming industry has an annual breeding capacity of nearly 2,000 tons. Wastes such as fish skin and fish scales account for 20%, and about 370 tons of waste cannot be disposed of, which has brought certain damage to the environment. pressure

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  • Process for extracting active collagen peptide from salmon trout waste

Examples

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Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 A process for extracting active collagen peptides from salmon and trout waste, comprising the following steps:

[0050] (1) Material selection and pretreatment:

[0051] Skin and bones from salmon trout trimmings are selected and separated; skin and bones are then pre-treated.

[0052] Fish skin pretreatment—— first remove the flesh, scale and fins of the fish skin; then put it into a high-speed mixer and add NaCl with a mass concentration of 3%, and stir and clean it at a speed of 2000rpm; put it in for cleaning after 15 hours In the tank, add NaCl with a mass concentration of 0.9% at 4°C for 8-16 hours, stirring from time to time to remove impurity proteins; finally use ethyl acetate with a volume concentration of 10% at 4°C for 20-24 hours with intermittent stirring, and carry out skim.

[0053] Wherein: the mass volume ratio of fish skin and 3% NaCl is 1: 20; the mass volume ratio of fish skin and 0.9% NaCl is 1: 10; the mass volume ratio of fish skin an...

Embodiment 2

[0069] Example 2 A process for extracting active collagen peptides from salmon and trout waste, comprising the following steps:

[0070] (1) Material selection and pretreatment:

[0071] Skin and bones from salmon trout trimmings are selected and separated; skin and bones are then pre-treated.

[0072] Fish skin pretreatment—— first remove the flesh, scale and fins of the fish skin; then put it into a high-speed mixer and add NaCl with a mass concentration of 3%, and stir and clean it at a speed of 2000rpm; put it in for cleaning after 15 hours In the tank, add NaCl with a mass concentration of 0.9% at 4°C for 8-16 hours, stirring from time to time to remove impurity proteins; finally use ethyl acetate with a volume concentration of 10% at 4°C for 20-24 hours with intermittent stirring, and carry out skim.

[0073] Wherein: the mass volume ratio of fish skin and 3% NaCl is 1: 30; the mass volume ratio of fish skin and 0.9% NaCl is 1: 20; the mass volume ratio of fish skin an...

Embodiment 3

[0089] Example 3 A process for extracting active collagen peptides from salmon and trout waste, comprising the following steps:

[0090] (1) Material selection and pretreatment:

[0091] Skin and bones from salmon trout trimmings are selected and separated; skin and bones are then pre-treated.

[0092] Fish skin pretreatment—— first remove the flesh, scale and fins of the fish skin; then put it into a high-speed mixer and add NaCl with a mass concentration of 3%, and stir and clean it at a speed of 2000rpm; put it in for cleaning after 15 hours In the tank, add NaCl with a mass concentration of 0.9% at 4°C for 8-16 hours, stirring from time to time to remove impurity proteins; finally use ethyl acetate with a volume concentration of 10% at 4°C for 20-24 hours with intermittent stirring, and carry out skim.

[0093] Wherein: the mass volume ratio of fish skin and 3% NaCl is 1: 25; the mass volume ratio of fish skin and 0.9% NaCl is 1: 15; the mass volume ratio of fish skin an...

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Abstract

The invention relates to a process for extracting active collagen peptide from salmon trout waste, comprising the following steps of: (1) selecting salmon trout skin and bones in salmon trout waste, separating the salmon trout skin and bones, respectively pre-treating the salmon trout skin and bones, washing and drying to obtain fresh raw materials of salmon trout skin and bones; (2) respectively carrying out enzyme coupling hydrolysis reaction on the fresh raw materials of salmon trout skin and bones to obtain coupled supernatants; (3) purification and concentration of target collagen peptide; respectively and sequentially carrying out the micro-filtration and the ultrafiltration-concentration on the supernatants to respectively obtain concentrated solutions of the salmon trout skin and the salmon trout bones; and (4) preparation of the target collagen peptide powder: respectively drying and screening the salmon trout skin salmon trout bones to obtain the salmon trout skin collagen peptide and the salmon trout bone collagen peptide. The method is simple and reduces the energy consumption, and the obtained products have higher purity, total anti-oxidation activity and nutritive value.

Description

technical field [0001] The invention relates to a process for extracting active collagen peptides, in particular to a process for extracting active collagen peptides from salmon and trout waste. Background technique [0002] Bioactive collagen peptides refer to peptide compounds that are beneficial to the life activities of organisms or have physiological effects, including endogenous and exogenous bioactive peptide collagen peptides. Endogenous bioactive peptide collagen peptide mainly exists in skin, muscle, bone, tooth, internal organs, (such as stomach, intestine, heart, lung), blood vessels, esophagus and eyes and other tissues and organs. An indispensable ingredient for tissues, rich in 18 kinds of amino acids, with a molecular weight of 1000-3000 Da, very good digestion and absorption, strong skin protection, antibacterial, immune, anti-oxidation, anti-hypertensive, cholesterol-lowering, mineral-binding, and visceral strengthening organ, anti-aging and other function...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 高天鹏王东徐红伟何延忠孙慧丽
Owner LANZHOU CITY UNIV
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