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Bacillus subtillis and method for preparing raw powder of each gram of bacillus subtillis with 1 trillion live germs

A technology of Bacillus subtilis and spores, which is applied in the field of feed additives and aquaculture, and can solve the problems of difficult control of bacterial content, easy contamination by miscellaneous bacteria, unstable product quality, etc.

Active Publication Date: 2011-01-05
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid fermentation method is to inoculate probiotics on solid medium for fermentation and cultivation. Its advantages are relatively extensive management, simple production process, and low investment. Disadvantages of unstable product quality

Method used

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  • Bacillus subtillis and method for preparing raw powder of each gram of bacillus subtillis with 1 trillion live germs

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Embodiment Construction

[0041] A kind of preparation method of every gram of bacillus subtilis former powder of 1,000 billion live spores, its step is:

[0042] 1. Screening of BsKN-48 strain:

[0043] Collect samples from different soils and water bodies across the country, weigh 1.0g of the sample into a small triangle flask on a sterile ultra-clean bench, add 9mL of sterile water, shake it fully and let it stand, take the supernatant as a concentration gradient Dilution (1×10 -4 , 1×10 -5 ,, 1×10 -6 , 1×10 -7 , 1×10 -8 ). Use a pipette gun to draw 100ul of the diluted solution and spread it evenly on A 1 Separation medium (bean cake powder 30g, starch 8g, yeast powder 10g, dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.3g, manganese sulfate 0.05g, calcium carbonate 0.1g, agar 15g, water 1000mL, pH 7.5, extinguished at 121°C Bacteria 30min), after culturing in a temperature-controlled incubator at 30°C for 3-5 days, pick out the strains with high spore synchronization rate (spore f...

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Abstract

The invention discloses bacillus subtillis and a method for preparing raw powder of each gram of bacillus subtillis with 1 trillion live germs. The bacillus subtillis is CCTCC: No. M209317. The method comprises the following steps of: A, screening of a Bs strain: collecting a sample from soil and water, adding sterile water into the sample and standing the sample; B, confirmation of the Bs strain: together measuring 1,244 effective basic groups in 16SrDNA of the stain; C, screening of Bs culture medium: determining an optimal factor from numerous carbon sources, nitrogen sources and trace element factors by adopting a response surface experiment of Box-Behken design and using the germ number as a screening index; D, improving the quantity of fermentation organisms, and performing batch fermentation by using the culture medium screened by a response surface method; E, extracting and reclaiming the germs; and F, detecting the number of the live germs, namely accurately detecting the number of the effective live germs in the product. The method has the advantages of good repeatability, low cost, capability of simultaneously detecting 7 to 8 samples and simple operation. The produced bacillus subtillis has the advantages of clean and safe raw medicament, high reclamation rate, stable germ number of the product, capability of reaching 1 trillion live germs in each gram and the like.

Description

technical field [0001] The invention relates to the fields of feed additives and aquaculture, and more specifically relates to a preparation method of bacillus subtilis raw powder (1,000 billion live spores / gram), which will be widely used in the feed industry and aquaculture. As the function of bacillus is still being discovered, the scope of application is also expanding. This product will be applied to new fields such as purification of polluted water, fruit preservation, garbage deodorization, etc., and has strong competitiveness in the domestic and international markets. . Background technique [0002] Since the 1950s, antibiotics have played a major role as disease treatments and animal growth promoters. However, due to the increasingly serious negative effects of antibiotics, the World Health Organization has banned the use of any antibacterial drugs in feed, and some developed countries have clear regulations on the use of veterinary antibiotics. Feed and aquacultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A61K35/74A61P1/00A61P31/04A61P37/04C12R1/125A23K10/18A61K35/742
Inventor 李青刘华梅陈振民杨克华谢翔程治国陈又香余娜王巧梅黄志远龚永华谢攀李坤闫世君
Owner WUHAN KERNEL BIO-TECH CO LTD
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