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Producing strain and production method for producing microbial flocculant from wheat starch waste water

A technology of microbial flocculant and wheat starch, which is applied in the field of waste and sewage treatment and disposal, can solve the problems of high cost of culture medium, high cost of microbial flocculant, low synthesis efficiency, etc., and achieves short floc production cycle and is suitable for large-scale industrialization. Production and promotion, the effect of reducing production costs

Inactive Publication Date: 2011-01-12
无锡天易生物科技有限公司 +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although microbial flocculants have the above advantages, due to the high cost of their culture medium and low synthesis efficiency, the cost of microbial flocculants remains high. Most of the research on microbial flocculants is still in the stage of laboratory research and development.

Method used

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  • Producing strain and production method for producing microbial flocculant from wheat starch waste water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Wheat starch wastewater was obtained from Jiangsu Lianhai Biotechnology Co., Ltd., with a CODcr of 9620 mg / L and a pH of 4.25.

[0023] (1), seed liquid culture:

[0024] (a) Seed culture solution: take an appropriate amount of wheat starch wastewater, add corn steep liquor to the wheat starch wastewater at a ratio of 10 g / L, then adjust the pH to 6.5 with ammonia water, and sterilize at 121° C. for 20 minutes;

[0025] Culture conditions: Pick the production bacteria (Bacillus-TY35) preserved on the inclined plane, inoculate them in the seed culture solution, and cultivate them at 35°C for 20 hours, with the shaker speed at 180r / min, until the bacterial concentration is about 1.0×10 8 CFU / mL, this is used as the seed solution during inoculation;

[0026] (2) Fermentation production of flocculant:

[0027] (a) Liquid fermentation medium: put 3.5 liters of wheat starch wastewater into a 5-liter fermenter, add corn steep liquor to the wheat starch wastewater at a ratio ...

Embodiment 2

[0036] The CODcr of wheat starch wastewater is 18500mg / L, and the pH value is 4.10.

[0037] Wheat starch wastewater was obtained from Jiangsu Lianhai Biotechnology Co., Ltd., with a CODcr of 18500 mg / L and a pH of 4.10.

[0038] (1), seed liquid culture:

[0039] (a) Seed culture solution: take an appropriate amount of wheat starch wastewater, add corn steep liquor to the wheat starch wastewater at a ratio of 15g / L, then adjust the pH to 6.5 with ammonia water, and sterilize at 121°C for 20 minutes;

[0040] Culture conditions: Pick the production bacteria (Bacillus-TY35) preserved on the slant, inoculate it in the seed culture solution, and cultivate it for 15 hours at 36°C with a shaker speed of 180r / min until the bacterial concentration is about 2×10 8 CFU / mL, this is used as the seed solution during inoculation;

[0041] (2) Fermentation production of flocculant:

[0042] (a) Liquid fermentation medium: put 3.5 liters of wheat starch wastewater into a 5-liter fermenter...

Embodiment 3

[0048] Wheat starch wastewater was obtained from Jiangsu Lianhai Biotechnology Co., Ltd., with a CODcr of 28500 mg / L and a pH of 4.0.

[0049] (1), seed liquid culture:

[0050] (a) Seed culture solution: take an appropriate amount of wheat starch wastewater, add corn steep liquor to the wheat starch wastewater at a ratio of 20 g / L, then adjust the pH to 6.5 with ammonia water, and sterilize at 121° C. for 20 minutes;

[0051] Culture conditions: Pick the production bacteria (Bacillus-TY35) preserved on the slant, inoculate it in the seed culture solution, and cultivate it at 36°C with a shaker speed of 180r / min for 14h until the bacterial concentration is about 5×10 8 CFU / mL, this is used as the seed solution during inoculation;

[0052] (2) Fermentation production of flocculant:

[0053] (a) Liquid fermentation medium: 3.0 liters of wheat starch wastewater was loaded into a 5-liter fermenter, corn steep liquor was added to the wheat starch wastewater at a ratio of 3.0 g / L,...

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Abstract

The invention relates to a producing strain for producing microbial flocculant from wheat starch waste water and a production method thereof. The producing strain is Bacillus sp.-TY35 which is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2010166. The production method comprises the following steps: the culture of a seed liquid, the fermentation and the production of the flocculant, and the extraction of the flocculant. By using low-cost starch wastewater as the culture medium to prepare the microbial flocculant, the producing strain effectively uses the wheat starch waste water, saves the carbon source cost in the traditional culture medium, and greatly reduces the production cost of the microbial flocculant. The production method for producing the microbial flocculant has the advantages of high yield, short flocculant-producing cycle and wide application range, and has favorable turbidity removal and COD removal effects on the waste water.

Description

technical field [0001] The invention belongs to the technical field of waste water treatment and disposal in environmental engineering, and relates to the production and preparation of microbial flocculants, in particular to a production bacterium for producing microbial flocculants by utilizing wheat starch wastewater and a production method thereof. Background technique [0002] At present, the commonly used treatment methods for food and fermentation industrial wastewater and domestic sewage at home and abroad can be generally divided into biological methods, physical methods and chemical methods. The small particles of pollutants are generally destabilized by adding flocculants, aggregated into large particles of alum that can be precipitated, and then removed. Flocculants can significantly improve the sedimentation speed and treatment effect. As a low-cost water treatment method, the flocculation and sedimentation method is widely used and occupies an extremely importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/07
Inventor 周洪余晓斌刘和
Owner 无锡天易生物科技有限公司
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