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Method for preparing butanol fermentation culture medium

A butanol fermentation and culture medium technology, which is applied in the field of preparation of butanol fermentation medium, can solve the problems of high price and achieve the effects of reducing raw material costs, good economic benefits and society, and reducing pollution

Inactive Publication Date: 2011-01-12
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fermentation of butanol using five-carbon sugars that have been reported at home and abroad are all chemically synthesized media, and the price is relatively expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Corn stalks are pulverized and put into a hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:6, hydrolyze at 130°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain hydrolyzed slag and hydrolyzed solution. The straw hydrolyzed solution is passed through a DSA V-type electrodialysis device and an alkaline anion resin exchange device to remove acid radical ions, and obtain straw xylose with a five-carbon sugar concentration of 4-10% as a carbon source The concentration of sulfurous acid in the corn extract after flashing is 0.05g / L, the corn extract after flashing is mixed with the cornstarch milk with the ratio of 5: 1 as nutrient substance, add to straw five-carbon sugar liquid As the fermentation medium, the mass ratio of nutrients to straw pentoses was 1:10; the fermentation medium was adjusted to pH 6.5 with NaOH, sterilized at 121°C for 10 minutes, and...

Embodiment 2

[0020] Corn stalks are crushed and put into the hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:5, hydrolyze at 120°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain the hydrolyzed slag and the hydrolyzate, and the straw hydrolyzate passes through the DSA V-type electrodialysis device and the basic anion resin exchange device to remove the acid radical ions, and obtain straw xylose with a five-carbon sugar concentration of 6% as a carbon source; The concentration of sulfurous acid in the steamed corn extract is 0.05g / L, and the flash steamed corn extract and corn starch milk are mixed in a ratio of 3:1 as nutrients, and added to the straw five-carbon sugar solution as Fermentation medium, the mass ratio of nutrients and straw pentoses is 1:50; adjust the fermentation medium to pH6.5 with NaOH, sterilize at 121°C for 10min, add 8% acetone butanol seed solutio...

Embodiment 3

[0022] Corn stalks are crushed and put into a hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:5, hydrolyze at 130°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain hydrolyzed slag and hydrolyzed solution. The straw hydrolyzed solution is passed through a DSAV type electrodialysis device and an alkaline anion resin exchange device to remove acid radical ions, and obtain straw xylose with a pentasaccharide concentration of 4-10% as a carbon source; The concentration of sulfurous acid in the corn extract after flashing is 0.05g / L, mix the corn extract after flashing with cornstarch milk at a ratio of 1:1 as nutrients, and add it to the straw five-carbon sugar liquid As a fermentation medium, the mass ratio of nutrients to straw pentoses is 1:10; the fermentation medium is adjusted to pH6.5 with NaOH, sterilized at 121°C for 10min, and 8% acetone-butanol seed so...

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PUM

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Abstract

The invention relates to a method for preparing a butanol fermentation culture medium. The method comprises the following step of: fermenting with Clostridium. acetobutylicum to obtain 1.8 to 2.3 percent total solvent by using pentose obtained by hydrolyzing straws with dilute acid as a carbon source, and corn steep liquor and starch milk as nutritive materials. By the culture medium provided by the invention, the butanol fermentation meets the requirement of industrial production and the butanol is produced by fermenting the cheap straws and the corn steep liquor serving as an industrial waste material instead of the conventional food raw materials or a chemosynthesis culture medium, so that the cost of the raw materials is reduced, the pollution to environment caused by burning of the straws and the corn steep liquor is also reduced, and the method has great economic benefit and social benefit.

Description

technical field [0001] The method belongs to the field of fermentation industry, in particular to a preparation method for butanol fermentation medium. Background technique [0002] Butanol is an important solvent and the main raw material for coatings, plastics, rubber and other products. Butanol is also a promising new biofuel. Compared with methanol and ethanol, butanol is closer to gasoline in performance, its calorific value and octane number are equivalent to gasoline, and it can be mixed with gasoline in any ratio. The biological fermentation of butanol is generally carried out under anaerobic conditions by using Clostridium.acetobutylicum, and its main products are acetone, butanol, and ethanol, which are collectively referred to as the total solvent of acetobutylicum. Acetone butanol fermentation was once second only to ethanol fermentation in terms of production scale. However, since the 1960s, due to the competition of chemically synthesized butanol, the butano...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12R1/145
Inventor 陈洪章王岚马保华生晓东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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