Method for preparing butanol fermentation culture medium
A butanol fermentation and culture medium technology, which is applied in the field of preparation of butanol fermentation medium, can solve the problems of high price and achieve the effects of reducing raw material costs, good economic benefits and society, and reducing pollution
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Embodiment 1
[0018] Corn stalks are pulverized and put into a hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:6, hydrolyze at 130°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain hydrolyzed slag and hydrolyzed solution. The straw hydrolyzed solution is passed through a DSA V-type electrodialysis device and an alkaline anion resin exchange device to remove acid radical ions, and obtain straw xylose with a five-carbon sugar concentration of 4-10% as a carbon source The concentration of sulfurous acid in the corn extract after flashing is 0.05g / L, the corn extract after flashing is mixed with the cornstarch milk with the ratio of 5: 1 as nutrient substance, add to straw five-carbon sugar liquid As the fermentation medium, the mass ratio of nutrients to straw pentoses was 1:10; the fermentation medium was adjusted to pH 6.5 with NaOH, sterilized at 121°C for 10 minutes, and...
Embodiment 2
[0020] Corn stalks are crushed and put into the hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:5, hydrolyze at 120°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain the hydrolyzed slag and the hydrolyzate, and the straw hydrolyzate passes through the DSA V-type electrodialysis device and the basic anion resin exchange device to remove the acid radical ions, and obtain straw xylose with a five-carbon sugar concentration of 6% as a carbon source; The concentration of sulfurous acid in the steamed corn extract is 0.05g / L, and the flash steamed corn extract and corn starch milk are mixed in a ratio of 3:1 as nutrients, and added to the straw five-carbon sugar solution as Fermentation medium, the mass ratio of nutrients and straw pentoses is 1:50; adjust the fermentation medium to pH6.5 with NaOH, sterilize at 121°C for 10min, add 8% acetone butanol seed solutio...
Embodiment 3
[0022] Corn stalks are crushed and put into a hydrolysis tank, add 0.3% dilute sulfuric acid, the ratio of straw to dilute sulfuric acid is 1:5, hydrolyze at 130°C for 2 hours, and then use a screw extruder to separate the solid and liquid in the hydrolyzed solution Separate to obtain hydrolyzed slag and hydrolyzed solution. The straw hydrolyzed solution is passed through a DSAV type electrodialysis device and an alkaline anion resin exchange device to remove acid radical ions, and obtain straw xylose with a pentasaccharide concentration of 4-10% as a carbon source; The concentration of sulfurous acid in the corn extract after flashing is 0.05g / L, mix the corn extract after flashing with cornstarch milk at a ratio of 1:1 as nutrients, and add it to the straw five-carbon sugar liquid As a fermentation medium, the mass ratio of nutrients to straw pentoses is 1:10; the fermentation medium is adjusted to pH6.5 with NaOH, sterilized at 121°C for 10min, and 8% acetone-butanol seed so...
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