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Bacillus megaterium and application thereof to printing and dyeing wastewater treatment

A technology of Bacillus megaterium, printing and dyeing wastewater, applied in textile industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc. Effect

Inactive Publication Date: 2012-04-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the adsorption of reactive dyes by bacteria. At present, only Corynebacterium glutamicum (Corynebacterium glutamicum) has a better effect.
However, almost all of these microorganisms achieve the maximum decolorization rate under low pH conditions, which is not conducive to the wide application of biosorbents in the treatment of printing and dyeing wastewater.

Method used

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  • Bacillus megaterium and application thereof to printing and dyeing wastewater treatment
  • Bacillus megaterium and application thereof to printing and dyeing wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening of Bacillus megaterium YL1

[0020] Bacillus megaterium (Bacillus megaterium) YL1 was isolated from the bottom mud of Taihu Lake. The formula of the YPD plate screening medium is: taking 1L medium as an example, it contains 20g of peptone, 20g of yeast powder, 10g of glucose, 50.1g of active blue, 16g of agar, the balance is water, and the initial pH of the medium is 5.8. The specific screening method is as follows:

[0021] (1) Take 5g of bottom mud in a 150mL Erlenmeyer flask with 50mL of sterile water, take it out after 3h at a shaking table speed of 100rpm, and then dilute the diluted bottom mud to 10 -1 , 10 -2 , 10 -3 , 10 -4 and 10 -5 , spread to the YPD plate culture medium with a glass rod;

[0022] (2) Inoculate the bacteria grown in the YPD plate medium to the YPD plate screening medium added with reactive blue 5, and screen out the strains with high dye adsorption ability according to the size of the transparent circle.

[0023] Th...

Embodiment 2

[0024] The preparation method of embodiment 2 microbial adsorbent

[0025] Bacillus megaterium YL1 is activated and fermented first, and the activation and fermentation medium are both YPD medium (that is, 1L medium contains 20g of peptone, 20g of yeast powder, 10g of glucose, and the balance is H 2 O, pH 5.8). When activated, the strain YL1 was inserted into the YPD medium according to the inoculation amount of 1wt%, and cultured at 150rpm at 28°C for 12h to enter the logarithmic growth phase, and then 15mL of it was transferred into a Erlenmeyer flask containing 150ml of YPD medium, and cultured at 28°C 48h, then centrifuged at 6000rpm for 10min to collect the bacterial cells, washed with sterile water after collecting the bacterial cells, and repeated 2 times. Finally, the bacteria are treated with a vacuum freeze dryer for 48 hours to obtain the microbial adsorbent of the present invention.

[0026] The following methods are used to determine the components and content i...

Embodiment 3

[0031] Example 3 Application of microbial adsorbent in the treatment of printing and dyeing wastewater containing reactive blue 5

[0032] Preparation of printing and dyeing wastewater containing reactive blue 5: add reactive blue 5 to tap water and stir evenly, wherein the concentration of reactive blue 5 is 0.1g / L.

[0033] The specific method is as follows: according to the addition amount of 2.5g / L, the microbial adsorbent obtained in Example 2 was added to the above-mentioned printing and dyeing wastewater containing reactive blue 5, and treated at room temperature and under aerobic conditions for more than 12h.

[0034] After 12 hours of decolorization treatment according to the above method, the decolorization effect of the microbial adsorbent on the artificially prepared dye wastewater was measured. 600 Measure its absorbance.

[0035] It has been determined that the decolorization rate of the novel microbial adsorbent to printing and dyeing wastewater containing reac...

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Abstract

The invention discloses Bacillus megaterium YL1 and application thereof to printing and dyeing wastewater treatment. In the Bacillus megaterium YL1, the collection number is CGMCC No.4094; the culture temperature is between 28 and 50 DEG C; the pH value is 5.8 to 7.2; and the culture medium is a YPD culture medium. The Bacillus megaterium YL1 is a microbial adsorbent of active ingredients and can effectively remove the dye components in the printing and dyeing wastewater so as to control the damage of the massive discharge of the printing and dyeing wastewater to the ecological system. When reactive blue 5-containing printing and dyeing wastewater is treated, the dyeing removing effect is basically not influenced by the pH value of the printing and dyeing wastewater and metal ions contained by the printing and dyeing wastewater. When the using amount of the adsorbent reaches 2.5 grams per liter, the maximum adsorbing effect can be achieved and the dyeing removing rate is 95 percent. A research on the adsorption process of the adsorbent at different temperatures shows that when delta G is a negative value in the adsorption process, the whole adsorption process is spontaneous. The effect of treating the printing and dyeing wastewater cannot be influenced, even if the adsorbent is stored for 6 months.

Description

technical field [0001] The invention belongs to the field of printing and dyeing wastewater treatment, in particular to a bacillus megaterium and its application in the treatment of printing and dyeing wastewater. Background technique [0002] The dye industry is one of the most serious environmental pollution industries in the chemical industry. Printing and dyeing wastewater has high chroma, high concentration of organic matter, complex components, many refractory biodegradable substances, and a large amount of inorganic salts, sulfides, etc., which are difficult to treat industrial wastewater. According to the survey of the International Dyestuff Manufacturing Industrial Ecology Association (ETAD), more than 80% of the more than 30,000 synthetic dyes used in the world are complex organic compounds containing azo bonds and polyaromatic rings; most of the dyes are toxic and difficult to produce. It degrades organic matter, has strong chemical stability, and has carcinogeni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01J20/00C02F3/34C12R1/11C02F103/30
Inventor 杨金水刘辰袁红莉李宝珍
Owner CHINA AGRI UNIV