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Method for preparing oligoxylose containing ferulic acid

A technology of xylo-oligosaccharides and ferulic acid, applied in the direction of fermentation, can solve problems such as no, and achieve the effect of improving competitiveness

Active Publication Date: 2011-01-19
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extract ferulic acid from these seed husks, there are many research reports at home and abroad, but there is no report at home and abroad to produce xylo-oligosaccharides containing ferulic acid with corn bran, wheat bran, and tea dregs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: corn bran system contains the xylo-oligosaccharide of ferulic acid

[0026] (1) Pretreatment: Add water to corn husks under normal pressure and boil at 100°C for 1 hour to convert starch into dextrin for dissolution, wash away, and then use it as a starting material.

[0027] (2) Enzymolysis: first use acetic acid to adjust the pH of the washed corn bran to 4.9, then add the prepared xylanase solution (1g xylanase dissolved in 100ml of water), incubated at 50°C and 180r / min in a shaker for 10 hours, and then filtered out the enzymolysis solution.

[0028] (3) Decolorization: Take 300ml of corn husk enzymatic hydrolysis solution, the solid content is 1.3%, add activated carbon for sugar at 0.5% of the liquid volume, and decolorize at 80°C for 30min. Then filter out the activated carbon therein.

[0029] (4) Concentration: after decolorization, the enzymolysis solution was concentrated to 3% solids with a rotary evaporator,

[0030] (5) Ion exchange: the ...

Embodiment 2

[0032] Embodiment 2: the xylo-oligosaccharide containing ferulic acid is made from tea dregs

[0033] (1) Pretreatment: Take 30 g of tea dregs containing about 25% protein, add 300 ml of water, adjust the pH to 8.0 with NaOH, and keep warm for 10 minutes in a water bath at 40°C, then separate the dregs from the liquid, and take the dregs as the starting material.

[0034] (2) Enzymolysis: adjust the pH of the pretreated tea leaves with acetic acid to be 4.8, then add the prepared xylan enzyme solution (1g xylan Dissolve enzyme in 100ml water), incubate at 50°C and 180r / min shaker for 10 hours, then filter out the enzymolysis solution.

[0035] (3) Decolorization: 300 ml of tea residue enzymatic hydrolysis solution, 0.5% of solid content. Add activated carbon for sugar according to 0.5% of the liquid volume, and decolorize at 80°C for 30min. Then filter out the activated carbon therein.

[0036] (4) Concentration: 300 ml of the enzymolysis solution after decolorization was c...

Embodiment 3

[0039] Embodiment 3: Wheat bran system contains the xylo-oligosaccharide of ferulic acid

[0040] (1) Bran pretreatment: Similar to corn bran, add water under normal pressure and boil at 101°C for 1 hour to convert starch into dextrin and dissolve it, wash it away, and then use it as a starting material.

[0041] (2) Enzymolysis: adjust the pH of the pretreated bran to 5.0 with acetic acid, then add the prepared xylanase solution (1g xylanase solution in the ratio of dry basis bran: enzyme = 1g: 30μg) in 100ml of water), 50°C, 180r / min shaker for 10 hours, then filter out the enzymolysis solution.

[0042] (3) Decolorization: Take 300ml of bran enzymatic hydrolysis solution, with a solid content of 2%, add activated carbon for sugar at 0.5% of the liquid volume, and decolorize at 80°C for 30min. Then filter out the activated carbon therein.

[0043] (4) Concentration: after decolorization, the enzymolysis solution was concentrated to 3% solids with a rotary evaporator,

[0...

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Abstract

The invention provides a method for preparing oligoxylose containing ferulic acid, which comprises the following steps of: (1) pretreatment: removing starch or protein impurities from raw materials to obtain initial raw materials; (2) enzymolysis: adding a proper amount of water into the initiative raw materials, then adjusting the pH to be between 4.8 and 5.0, adding 0.8 to 1.2 percent xylanase solution into the solution in a ratio of dry basis initial raw materials to enzyme of 1g: 25-35mug, performing enzymolysis for 10 to 12 hours at the temperature of between 45 and 55 DEG C, and filtering the enzymolysis solution; (3) concentration: evaporating and concentrating the enzymolysis solution till the solid content is 2.5 to 3.5 percent; and (4) ion exchange: performing ion exchange purification on the concentrated enzymolysis solution through positive-negative-positive resin, wherein the discharged solid is 0.9 to 1.1 percent. Preferably, the enzymolysis solution is decolorized by using active carbon before concentration. Corn cobs are replaced by industrial byproducts such as corn peels, tea leaf residues, bran and the like; and pre-crushing and autoclaving with high energy consumption are not needed, so energy is saved. The obtained oligoxylose product contains the ferulic acid with anti-oxidation function, and becomes multifunctional oligoxylose.

Description

technical field [0001] The invention relates to a method for extracting xylo-oligosaccharides containing ferulic acid, in particular to a method for preparing xylo-oligosaccharides containing ferulic acid from industrial by-products such as corn bran, tea dregs, wheat bran and the like. Background technique [0002] Since the 1980s, the world, especially Japan, has developed various functional oligosaccharides that can adjust intestinal function and promote the proliferation of beneficial bacteria Bifidobacteria. In the mid-1990s, my country also realized the marketization of functional oligosaccharides. At present, the oligosaccharide varieties that are widely used and sold most in the domestic and foreign markets are: isomaltooligosaccharide made from starch (8,000 yuan per ton), and fructooligosaccharide made from sucrose (12,000 yuan per ton). The price is much lower than that of xylooligosaccharide (80,000-240,000 yuan per ton) prepared from corncobs. However, accordi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P7/42
Inventor 尤新肖林赵海涛葛青覃树林
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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