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Method for modifying air stability phospholipid membrane on solid surface

An air-stable, solid surface technology, applied in the field of surface chemistry and applied chemistry, can solve the problem of inability to modify air-stable phospholipid membranes, and achieve the effects of good air stability, shortened preparation time, and convenient operation.

Active Publication Date: 2011-01-26
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the deficiency that the existing air-stable phospholipid film technology cannot be modified on the surface of a solid substrate, and to provide a method for modifying an air-stable phospholipid film on the surface of a solid substrate

Method used

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  • Method for modifying air stability phospholipid membrane on solid surface
  • Method for modifying air stability phospholipid membrane on solid surface
  • Method for modifying air stability phospholipid membrane on solid surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare a chloroform solution of 1,2-dimyristoylphosphatidylcholine (1,2-Dimyristoylphophatidylcholine, DMPC), which contains 1,2-distearoyl-glycerol-3-phosphatidylethanolamine-N-carboxyl (polyethylene Diol 2000) (1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine-N-[Methoxy(Polyethyleneglycol)-2000], DSPE-PEG2000) were 0.1, 0.5, 1.5 mol%, respectively. Blow dry most of the chloroform with nitrogen, then vacuum dry for at least 2 hours to remove residual chloroform and form a phospholipid film on the container wall; add a certain amount of phosphate buffer (pH 7.4) to hydrate the film and make it The final concentration of DMPC was 1 mM. Ultrasonic treatment in a water bath for 30-60 min until the solution was clear; then the solution was extruded with Avanti's MiniExtruder, using a polycarbonate membrane with a pore size of 50 nm, and the number of extrusions was not less than 11.

[0029] Carry out amination modification on the surface of silicon wafers by conventional ...

Embodiment 2

[0033] Prepare a chloroform solution of 1,2-dioleoylphosphatidylcholine (Dioleoylphatidylcholine, DOPC), which contains 1mol% of DSPE-PEG 2000 First, dry most of the chloroform with nitrogen, and then vacuum-dry for at least 2 hours to remove residual chloroform and form a phospholipid film on the container wall; add a certain amount of phosphate buffer (pH 7.4) to hydrate the film, and The final concentration of DOPC was 1 mM. Ultrasonic treatment in a water bath for 30-60 min until the solution was clear; then the solution was extruded with Avanti's MiniExtruder, using a polycarbonate membrane with a pore size of 50 nm, and the number of extrusions was not less than 11.

[0034] The preparation steps of the "chitosan-modified glass surface" are the same as in Example 1.

[0035] The above-prepared small single-walled phospholipid vesicles and the chitosan-modified glass surface are allowed to stand for a reaction, and after 5-10 minutes at room temperature, the unbound exce...

Embodiment 3

[0037] Preparation of 1,2-dilauroylphosphatidylethanolamine (1,2-Dilauroyl-sn-Glycero-3-Phosphoethanolamine, DLPE) in chloroform, containing 2mol% of DSPE-PEG 2000 First, dry most of the chloroform with nitrogen, and then vacuum-dry for at least 2 hours to remove residual chloroform and form a phospholipid film on the container wall; add a certain amount of phosphate buffer (pH 7.4) to hydrate the film, and The final concentration of DLPE was 1 mM. Ultrasonic treatment in a water bath for 30-60 min until the solution was clear; then the solution was extruded with Avanti's MiniExtruder, using a polycarbonate membrane with a pore size of 50 nm, and the number of extrusions was not less than 11.

[0038] The preparation steps of the "chitosan-modified silica surface" are the same as in Example 1.

[0039] The above-prepared small single-walled phospholipid vesicles and the chitosan-modified silicon dioxide surface are allowed to stand for a reaction, and after 5-10 minutes at room...

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Abstract

The invention relates to the field of surface chemistry and applied chemistry, in particular to a method for modifying an air stability phospholipid membrane on a solid surface. The method comprises the following steps of: 1) preparing small single-wall phospholipid vesicle solution of poly(ethylene glycol) (PEG) modified phospholipid molecules, wherein the mole percentage of the PEG modified phospholipid molecules is no more than 5 mol percent; 2) performing standing reaction at room temperature between the small single-wall phospholipid vesicle solution obtained in the step 1) and the chitosan modified solid surface; and 3) removing unbonded phospholipid vesicle. The method can solve the problem of air instability when the phospholipid membrane is modified by the conventional method; and due to the addition of the PEG modified phospholipid, the preparation time for the small single-wall phospholipid vesicle is greatly shortened and the stability of the phospholipid vesicle is improved. The phospholipid bilayer formed by the method has the advantages of good air stability, convenient operation and good repeatability. If 2 to 3 mol percent PEG modified phospholipid molecules are added, the single phospholipid bilayer with the air stability can be modified.

Description

technical field [0001] The invention relates to the fields of surface chemistry and applied chemistry, in particular, the invention relates to a method for modifying an air-stable phospholipid film on a solid surface. Background technique [0002] Biochips and their applications are current research hotspots. After biomolecules are immobilized on the chip surface, there is often the possibility that the biological activity will be reduced or even lost; in addition, the surface of the chip often causes non-specific adsorption of proteins. important hurdles in application. The biofilm is a natural structure that maintains the activity of biomolecules and has the characteristics of preventing protein adsorption; if the bionic principle is used to modify the artificial biofilm on the surface of the biochip, that is, to modify the phospholipid film layer similar to the biofilm, it can provide biological The environment in which molecules maintain and exert biological activity ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B41/48C03C17/28
Inventor 张义浜陈艳艳靳刚
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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