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Lactobacillus reuteri and composite viable bacteria preparation thereof

A technology of Lactobacillus reuteri and bacteria agent, applied in the field of microecological preparations, can solve the problems of application effects, etc., and achieve the effects of increasing daily weight gain, reducing diarrhea rate of piglets, and improving feed efficiency

Inactive Publication Date: 2011-02-16
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the production strains of compound living bacteria preparations used in pigs are non-pig-derived probiotics. Since the formation of animal intestinal flora is the result of long-term evolution, a microecological preparation suitable for a certain type of animal may not be Suitable for another type of animal, resulting in a greater impact on the application effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Preparation of Lactobacillus reuteri live bacteria preparation

[0028] Activation of strains

[0029] Streak inoculation of Lactobacillus reuteri L-HD5 on the surface of MRS solid medium (manufactured by Beijing Land Bridge Technology Co., Ltd., batch number: CM188), cultured anaerobically at 37°C for 16-24h, Until bacterial colonies grow on the surface of the solid medium, observe the morphology and purity of the bacteria by microscopic examination.

[0030] Preparation of seed solution

[0031] Pick the colony on the surface of the MRS solid medium, inoculate it into a test tube containing 5mL of seed medium, and incubate anaerobically at 37°C for 16-24 hours until the number of viable bacteria reaches 10 8 CFU / mL, according to 2% (about 1×10 6 CFU / mL) inoculum amount was inoculated into a 250mL Erlenmeyer flask containing 150mL seed medium, anaerobically cultured at 37°C for 16-24h, until the number of viable bacteria reached 10 8 CFU / mL, used as s...

Embodiment 2

[0041] Embodiment 2: the preparation of Bifidobacterium live bacteria preparation

[0042] Activation of strains

[0043] Freeze-dried strains of Bifidobacterium infantis (Bifidobacterium infantis, purchased from the General Microbiology Center of China Committee for Culture Collection of Microorganisms, No.: CGMCC No. 1.2202) were inoculated on the surface of MRS solid medium by streaking, and cultured anaerobically at 37°C for 16 ~24h, until colonies grow on the surface of the solid medium, observe the morphology and purity of the bacteria by microscopic examination.

[0044] Preparation of seed solution

[0045] Pick the colony on the surface of the MRS solid medium, inoculate it into a test tube containing 5mL of seed medium, and incubate anaerobically at 37°C for 16-24 hours until the number of viable bacteria reaches 10 8CFU / mL, according to 2% (about 1×10 6 CFU / mL) inoculum amount was inoculated into a 250mL Erlenmeyer flask containing 150mL seed medium, anaerobicall...

Embodiment 3

[0054] Embodiment 3: the preparation of bacillus natto live bacteria preparation

[0055] Activation of strains

[0056] The freeze-dried strains of Bacillus natto (purchased from the General Microbiology Center of China Microbiological Culture Collection Management Committee, number: CGMCC No.1.1086) were inoculated on nutrient agar medium (produced by Beijing Aoboxing Biotechnology Co., Ltd., batch number For: 20060808) surface, aerobic culture at 37°C for 12 to 24 hours until colonies grow on the surface of the culture medium, and the morphology and purity of the bacteria were observed by microscopic examination.

[0057] Preparation of seed solution Pick colonies on the surface of nutrient agar medium, inoculate them into a test tube containing 5 mL of nutrient broth medium (produced by Beijing Land Bridge Technology Co., Ltd., batch number: CM106), and incubate at 37°C for 12-24 hours. Bacteria up to 10 8 CFU / mL, according to 2% (about 1×10 6 CFU / mL) inoculum amount wa...

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Abstract

The invention provides Lactobacillus reuteri L-HD 5 with the preservation number of CGMCC No.4050 and a composite viable bacteria preparation thereof. The viable bacteria preparation of the invention serves as a feed additive, can obviously improve daily gain of weaned pigs and feed efficiency after being used for feeding, lowers piglet diarrhea rate and improves economic benefit. The invention has popularization and application prospect and provides certain theoretical and practical basis for searching an antibiotic succedaneum.

Description

technical field [0001] The invention relates to the technical field of microecological preparations, in particular to a Lactobacillus reuteri and a composite live bacteria preparation thereof. Background technique [0002] As a kind of microecological preparation, compound live bacteria preparation has been widely used in animal husbandry. It mainly consists of two or more different genera of probiotic bacteria. At present, the commonly used strains are mainly Lactobacillus, Bifidobacterium, Streptococcus faecalis, Bacillus and yeast. Combining probiotics with different biological functions, the compound live bacterial preparation developed can maintain the balance of the gastrointestinal environment through biological antagonism, biological oxygen capture and immune enhancement, and the probiotics can grow and metabolize in the animal body. The various digestive enzymes produced can significantly strengthen the body's digestion and absorption of various nutrients in the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12R1/225C12R1/07C12N1/20A23K1/16A23K10/18
Inventor 朱战波杨焕民刘宇孙秀军贾永全崔玉东张军
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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