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Method for producing pea peptide through enzyme method

A pea peptide and enzymatic technology, applied in the field of deep processing of pea protein powder, can solve the problems of no membrane separation technology, destroy protein components, and destroy products, and achieve the effects of no toxic side effects, easy absorption, and short production cycle.

Inactive Publication Date: 2011-02-16
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of alkali treatment is used in the extraction process, which not only pollutes the environment and poses safety hazards to the human body, but also destroys the protein components; while concentration is easy to destroy the product phase, and no membrane separation technology is used in the prior art, which affects the quality of the product

Method used

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  • Method for producing pea peptide through enzyme method
  • Method for producing pea peptide through enzyme method
  • Method for producing pea peptide through enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for enzymatically producing pea peptides, the steps are as follows:

[0023] Weigh 1kg of commercially available pea protein powder (where crude protein>61wt%, moisture<10wt%, ash<3wt%) after passing through a 40-mesh sieve, add 7kg of distilled water, 50g of 95wt% ethanol and 10g of anhydrous sodium sulfite, and stir After uniformity, heat up to 90°C and reflux for 5 hours, and filter while hot after the reaction; then cool the filtrate to 50°C, adjust the pH to 7-8 with 0.1M food-grade flaky sodium hydroxide solution, then add 20g of alkaline protease, Stir and enzymolyze at 50°C for 6 hours. During the enzymolysis process, use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system at 7-8; The sodium hydroxide solution of M keeps the pH value of the reaction system at 7-8; then the temperature is raised to 90° C., and the enzyme is kept for 20 minutes to kill the enzyme; then the enzymolysis solution is filtered to obta...

Embodiment 2

[0027] A method for enzymatically producing pea peptides, the steps are as follows:

[0028] Weigh 2kg of commercially available pea protein powder (where crude protein>61wt%, water content<10wt%, ash content<3wt%) after passing through a 40-mesh sieve, add 10kg distilled water, 200g 95wt% ethanol and 30g anhydrous sodium sulfite, stir After uniformity, heat up to 92°C and reflux for 4 hours, and filter while hot after the reaction; then cool the filtrate to 55°C, adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution, and then add 60g of alkaline protease, Stir and enzymolyze at 55°C for 5 hours. During the enzymolysis process, use the above-mentioned 0.1M sodium hydroxide solution to keep the pH value of the reaction system at 7-8; Sodium hydroxide solution to keep the pH value of the reaction system at 7-8; then raise the temperature to 94°C and keep it warm for 16 minutes to inactivate the enzyme; then filter the enzymolysis solution to obtain a supernatant; a...

Embodiment 3

[0033] A method for enzymatically producing pea peptides, the steps are as follows:

[0034] Weigh 10kg of commercially available pea protein powder (wherein crude protein>61wt%, water content<10wt%, ash content<3wt%) after passing through 40 mesh sieve, add 60kg distilled water, 700g 95wt% ethanol and 200g anhydrous sodium sulfite, After stirring evenly, raise the temperature to 93°C and reflux for 4 hours, filter while hot after the reaction; then cool the filtrate to 55°C, adjust the pH to 7-8 with 0.1M food-grade sodium hydroxide solution, and then add 400g of alkaline protease , stirred at 55°C for 5 hours, during the process of enzymolysis, use the above 0.1M sodium hydroxide solution to keep the pH of the reaction system at 7-8; then add 200g of flavor protease, and perform enzymolysis at 55°C for 6 hours, during the process of enzymolysis, use the above 0.1 The sodium hydroxide solution of M keeps the pH value of the reaction system at 7-8; then heats up to 96°C and in...

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Abstract

The invention discloses a method for producing a pea peptide through an enzyme method, comprising the following steps of: blending screened and edulcorated pea protein powder used as main raw materials with water, ethanol and sodium sulfite to react for 3-5 hours; filtering and cooling to 50-60 DEG C; then sequentially adding two proleases for enzymolysis; modifying; inactivating enzyme; decoloring; separating a membrane; and carrying out spray drying on obtained filter liquor to obtain the pea peptide. The method acquires the pea peptide through a biological double enzyme method, has the advantages of simple production process, short production period, low cost, easy and convenient operation, moderate reaction condition, environmental protection and high safety performance and can be widely used in the fields of drugs, health care products, and the like.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of pea protein powder, in particular to a method for enzymatically producing pea peptide, which uses pea protein powder as a raw material, uses biological enzymes to enzymolyze the pea protein powder, and then utilizes biological enzyme modification technology to produce pea peptides. A method for extracting pea peptides by modifying protein peptides. Background technique [0002] Pea grains contain 15.5%-39.7% protein, about 2% fat, and are rich in vitamins B1, B2 and niacin. Pea protein resources in my country are extensive and cheap. Pea peptides can be obtained by hydrolyzing pea protein, and the pH value is neutral. Studies have shown that pea peptide contains 8 amino acids that the human body cannot synthesize by itself, and their ratio is close to the recommended mode of FAO / WHO. [0003] However, since these amino acids basically exist in proteins in the form of agg...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14C12P21/06
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司
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