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Ningnanmycin composition and preparation method thereof

A technology of ningnanmycin and composition, applied in the directions of botanical equipment and methods, biochemical equipment and methods, microorganism-based methods, etc.

Active Publication Date: 2012-11-28
德强生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these reports went on to further study the characteristics of the fermentation of Streptomyces noursii var. Xichang and other fermentation products, especially those Ningnanmycin analogues with agricultural antibiotic potential or anti-bioaugmentant potential.

Method used

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  • Ningnanmycin composition and preparation method thereof
  • Ningnanmycin composition and preparation method thereof
  • Ningnanmycin composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 The preparation of the composition containing Ningnanmycin

[0052] Inoculate the frozen Streptomyces noursii var. Xichang (purchased from Heilongjiang Biopesticide Engineering Technology Research Center) on a freshly prepared solid medium, at 28 ° C, 80% relative humidity, and under the condition of sterile air circulation Cultured for 16 days for rejuvenation, and the resulting strain spores were picked. Then produce mycelium, that is, to inoculate the spores of the strain on the freshly prepared solid medium, and culture it aerobically at 28°C, 80% relative humidity, and under the condition of sterile air circulation for 14 days, and the mycelium can be clearly seen produce. Carry out proliferation culture then, promptly use the first freshly prepared liquid medium to wash mycelia from the surface of the solid medium and enter a 1L Erlenmeyer flask, add the first freshly prepared liquid medium to supplement to a medium volume of 250ml, Incubate for 14 ...

Embodiment 2

[0055] Example 2 Component analysis of compositions containing Ningnanmycin

[0056] According to the method of Example 1, 5 batches of compositions containing Ningnanmycin were prepared respectively, and component analysis was carried out.

[0057] (1) Determination of water content

[0058] Two samples were weighed for each batch, each about 0.1g (i.e., sample size), and placed in the SFY-3A type trace moisture analyzer (available from Shandong Zibo Hainuo Instrument Co., Ltd.) for detection. According to the water content obtained by the instrument, it is converted into water content. The results are shown in Table 2-1, all less than 0.5%.

[0059] Table 2-1 Sample moisture determination results of compositions containing Ningnanmycin

[0060]

[0061] (2) Determination of Ningnanmycin content

[0062] Referring to the manufacturer's instructions, use the Agilent 1100HP LC liquid chromatography in the Agilent 1100HP LC-MSD liquid chromatography-mass spectrometer to d...

Embodiment 3

[0072] Example 3 The control effect of the composition containing Ningnanmycin

[0073] We used the Ningnanmycin-containing composition (A) prepared according to Example 1 to dilute it with an equivalent amount of commercially available 2% Ningnanmycin (purchased from Heilongjiang Qianger Biochemical Technology Development Co., Ltd.) (B) for field preparation. Experimental comparison is used to show its control effect on various crops.

[0074] (1) Field efficacy test of rice stripe leaf blight

[0075] In Wuzhi County, Henan Province, the rice (Yujing No. 6) planted in the 1-mu rice test field (half of the area of ​​A and B were tested respectively, and the surrounding area without control was used as the blank control (C)) was tested on July 7 and The pesticides were applied once on July 14, and the effects of controlling rice stripe leaf blight were observed on the 7th and 14th days after the last application, respectively. The results are shown in Table 3-1 and 3-2 respe...

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Abstract

The invention provides a pesticide composition containing ningnanmycin. The pesticide composition also contains two ningnanmycin analogs which can effectively prevent and control plant virus diseases, fungus diseases and bacteria diseases separately and together. In addition, the invention also provides a preparation method for the pesticide composition, a prepared pesticide preparation and application of the ningnanmycin analogs and the like.

Description

technical field [0001] The invention belongs to the field of pesticides. Specifically, the invention relates to a pesticide composition containing Ningnanmycin, which is characterized in that it also includes two Ningnamycin analogs. In addition, the present invention also relates to the preparation method of the above-mentioned agricultural composition, the formulated pesticide preparation, the application of Ningnanmycin analogues, and the like. Background technique [0002] Ningnanmycin (ningnanmycin) is a kind of basic water-soluble cytosine nucleoside antibiotic isolated from Streptomyces noursei var.xichangenis (Slreptomyces noursei var.xichangenis) CGMCC No.0194. Shown), it has inhibitory effect on Gram-negative and positive bacteria, especially for rice bacterial blight, pathogenic bacteria of powdery mildew of plants such as wheat, cucumber, beans and flowers, and tobacco mosaic virus. (See Chinese Patent No. 93104287.9; Xiang Guxi, et al. A new agricultural antibi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/54A01N43/16A01P3/00A01P1/00C12P21/02C12R1/465
Inventor 孙新宇王敏刘伟徐艳吉
Owner 德强生物股份有限公司
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