Method for preparing large-head atractylodes rhizome and sesquiterpene compound

Abstract

The invention discloses a method for preparing a large-head atractylodes rhizome and sesquiterpene compound. The method comprises the following steps of: 1) extracting a large-head atractylodes rhizome medicinal material at a set temperature and under a set pressure in a supercritical extractor, and feeding the extract into a fractionating column along with supercritical CO2 fluid; 2) applying a temperature gradient from low to high to the fractionating column under a certain fractionation pressure to ensure that the supercritical CO2 fluid sectionally fractionates volatile oil at a fixed flow rate; and 3) separating the volatile oil subjected to fractionation into two fractionation sections including a monoterpene component and a sesquiterpene compound, performing pressure reduction and feeding into a separator sequentially, collecting the fractionation section of the sesquiterpene compound, metering the CO2 by a rotor meter and emptying. By the method, the supercritical extraction is coupled to fractionation technology, so that the removal of the terpene from the volatile oil of the large-head atractylodes rhizome in the supercritical CO2 medium and the enrichment of the sesquiterpene are continuously finished, and a new way is provided for preparing the monoterpene-free large-head atractylodes rhizome and sesquiterpene compound.

Description

technical field [0001] The invention belongs to the field of separation and purification of pharmaceutical components, and in particular relates to a method for preparing Atractylodes macrocephala sesquiterpene complex by supercritical extraction coupling fractionation technology. Background technique [0002] Sesquiterpenes are natural terpenoids with 15 carbon atoms in the molecule. The general formula is C 15 h 24 , with a molecular weight of 204. Sesquiterpenes often exist in volatile oils in the form of derivatives such as alcohols, ketones, and lactones in plants. They are the main components of high boiling point parts in volatile oils. Most of them have strong aroma and biological activity. important raw materials. [0003] Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala koidz, which is one of the most widely used medicinal materials in clinical practice. Traditional Chinese medicine believes that it is warm in nature, pungent and bitt...

AI Technical Summary

Problems solved by technology

The Chinese patent "Atractylodes ketone, plants containing atractylodes ketone and the use of atractylodes ketone extract in the preparation of anti-influenza virus drugs" (CN1857358A) discloses a method using supercritical CO 2 Method for extracting atractylodes ketone from atractylodes herb without removing monoterpene compounds therein

Although these patents use the supercritical extraction method, due to the supercritical CO 2 While extracting sesquiterpene components such as atractylone, some volatile small molecule monoterpene components are also extracted. If further purification is required, multiple column chromatography is involved, and the process is complicated

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