Rapid high-throughput screening model for antiviral drugs

Abstract

A establishment method of a novel high-throughput screening model is provided in the invention, which aims to solve the problems of nondeterminacy and incorrespondence exsiting in general in vitro high-throughput screening method. The model provided by the invention furthest simulates animal physiological environment, thus the accuracy of drug screening is increased and the results obtained by the method of in-vitro screening are closer to that of clinical application. The technical scheme of the present invention is as follows: Animal medicated serum is obtained by absorpting and separating drug active ingredients during the process of in vivo circulation of animals, thereby the medicated serum is added to cell culture media or maintenance media after drug is subject to the process of invivo metabolism of animals. Cell activity is calculated by utilizing micro cell culture method, cytopathic effects (CPE) measurement and MTT method.

Description

technical field [0001] The invention relates to the field of drug screening models, in particular to a method for establishing a rapid high-throughput screening model of antiviral drugs, which can be applied to drug screening. Background technique [0002] Drug screening model (drug screening assay method) is an experimental method used to prove that a certain substance has pharmacological activity (biological activity, therapeutic effect), and these experimental methods are one of the important conditions for finding and discovering drugs. In the long-term practice of searching for drugs, people have established a large number of various models for new drug screening, which can be roughly divided into classic animal experiment methods, molecular biology screening methods, high-throughput drug screening methods, and high-content drug screening techniques. [0003] The so-called animal screening method is to use animals as the observation objects of drug screening, to prove t...

AI Technical Summary

Problems solved by technology

However, the animal screening method also has disadvantages: when using animal models for drug screening, due to the particularity of animals, the drug screening process mainly depends on manual operations, and only limited samples can be screened, so that the overall animal model screening of new drugs has the advantages Obvious limitations, low efficiency, high cost, long time required for animal experiments, high labor intensity, high technical requirements for operation, large amount of test samples (about 5g)

The pharmacological effect of the drug is exerted through the drug target of the body cells or pathogens. Whether the drug for internal use is administered through any route of administration, such as oral administration or injection, it needs to go through the process of absorption, metabolism, distribution and excretion. Affect the effect of drugs, such as metabolism affects the molecular structure and effect of drugs through oxidation, decomposition, and combination. Enhancement, such as the metabolism of the inactive anticancer drug azathioprine to the pharmacologically active 6-thiopurine

Conventional in vitro cell or pathogen culture drug screening models, because the drug does not go through in vivo absorption, distribution, metabolism and other processes, but directly acts on cells or pathogens; therefore, it cannot truly reflect the role of the drug, and there is uncertainty and non-correspondence. Some drugs or drug ingredients that are effective in vitro experiments are not effective in clinical experiments

Usually, there is a big deviation between the results of in vitro drug screening and the clinical effect; at the same time, the conventional in vitro cell culture drug screening model needs to measure the safe dosage of cultured cells for each drug, which has the disadvantages of heavy workload, complicated operation and high cost.

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