Method for producing acid xylanase from whey powder

A technology of acid xylanase and whey powder, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of low enzyme activity, and achieve simple production process, fast breeding of strains, high activity high effect

Active Publication Date: 2011-04-13
湖南新鸿鹰生物工程有限公司
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AI Technical Summary

Problems solved by technology

At present, most scientific research institutes and enterprises mostly use corncobs, bran, straw, etc. as the main ra

Method used

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  • Method for producing acid xylanase from whey powder
  • Method for producing acid xylanase from whey powder
  • Method for producing acid xylanase from whey powder

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Embodiment Construction

[0037] Below in conjunction with accompanying drawing, the present invention is described in detail as follows:

[0038] 1. Bacteria culture:

[0039] (1) Strain selection: In the production of acid xylanase, Trichoderma reesei provided by Shandong Food Fermentation Industry Research and Design Institute was selected as the starting strain, and potato culture medium was used to make slanted seeds for future use.

[0040] (2) Seed mother shake flask culture: put 300ml of seed mother medium into a 1000ml Erlenmeyer flask, cool to room temperature after sterilization, insert a ring of slanted seeds, place on a shaking table at a speed of 220-250r / min, and cultivate at a temperature of 28 Incubate at ±1°C for 30±2 hours.

[0041] 2. Production seed culture:

[0042] (1) Primary seed cultivation:

[0043] 1. In 1M 3 The amount of seed culture medium added to the stainless steel seed tank accounted for 70-75% of the tank volume, steam sterilized at 121°C, tank pressure 0.1-0.12M...

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Abstract

The invention relates to a method for producing acid xylanase from whey powder. The method comprises the following steps of: culturing a strain in a shaking flask by taking trichoderma reesei as an original strain; performing primary seed culturing; performing secondary seed culturing; fermenting; performing primary filtration; performing secondary filtration; performing ultrafiltration and condensation; testing and blending; performing fine filtration and filling so as to obtain food grade liquid enzyme; drying and smashing a food grade liquid acid xylanase product by taking starch or bulked starch as an absorptive carrier so as to obtain a food grade solid acid xylanase preparation product; and spray-drying and smashing the food grade liquid acid xylanase product so as to obtain the food grade solid acid xylanase preparation product. The acid xylanase production process is simple, fast in strain reproduction, high in contaminated microbe resistance, and has short period, high equipment utilization ratio and low cost; and the obtained xylanase product has high activity. The xylanase, cellulose and beta-glucanase in the product have coordinated component content proportion and the product is most suitable for feed industry and beer industry.

Description

[0001] Field technology [0002] The invention relates to a production method of xylanase, in particular to a method for producing acid xylanase by using whey powder. Background technique [0003] Xylanase refers to the general term for a group of enzymes that can degrade xylan into oligosaccharides and xylose, mainly including β-1,4 xylanase and β-xylosidase, which can degrade xylanase in plants Degrade xylan into monosaccharides. Xylanase is a new type of enzyme preparation, which is currently mainly used in feed, beer, and other industries. [0004] As a feed additive, xylanase can effectively hydrolyze anti-nutritional factors such as non-starch polysaccharides and crack plant cell walls, reduce viscosity, promote the release of plant effective substances and the absorption of nutrients, enhance animal production capacity and disease resistance, and reduce livestock , Poultry morbidity, reduce pollution caused by animal excrement. Improve feed utilization and reduce feed...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/885
Inventor 李洪兵李海清朱永明胡永明易继云王永兰
Owner 湖南新鸿鹰生物工程有限公司
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