Method for extracting and separating xanthohumol and flavone compounds

Abstract

The invention relates to a method for extracting and separating xanthohumol and flavone compounds. The method comprises the following steps of: leaching hops and a supercritical CO2 extraction residue thereof with an alcohol solution to ensure that flavone compounds, particularly xanthohumol, are sufficiently dissolved out; filtering to obtain a filter liquor after leaching, and decompressing anddistilling the filter liquor to obtain a concentrated liquor; centrifuging the concentrated liquor, taking an obtained supernate, and filtering by using a 0.45muL of organic film; removing partial impurities, and eluting with an eluant to elute the flavone compounds from macroporous adsorption resin; collecting effluent liquid and concentrating; and freezing and drying to obtain yellow powder. In the invention, the hops, the supercritical CO2 extraction residue, and the like are used as raw materials, particularly, a waste material which is the supercritical CO2 hop extract extraction residuein a hop product processing process is used as the raw material, thus the xanthohumol which has the main efficacy and is difficult to separate is independently extracted, and other flavone compounds rich in the hop waste material are also extracted; and the invention also provides a development approach for hops which cannot be used for beer brewing, thereby improving the resource utilization ratio and the added value of the hops and having important significance to prolonging the hop industrial chain.

Description

technical field [0001] The invention belongs to the field of separation and purification of flavonoids, in particular to an extraction and separation method of xanthohumol and flavonoids. Background technique [0002] Hops (Humulus lupulus L), also known as Hubu, belong to the perennial herb of the family Moraceae, and are one of the main raw materials for brewing beer. It can make beer have a unique aroma and bitterness, as well as antiseptic effect. It can promote protein coagulation, is beneficial to the clarification of wort, and enhances the abiotic stability and foam retention of beer. Hops are also a medicinal and edible plant with a long history of use. Hops contain resins, hop oil, polyphenols, flavonoids and other pharmacological components. [0003] The hop products used in beer brewing can be divided into whole hops, hop powder, granular hops, hop extract and so on. The first three products all contain a large amount of flavonoids. The hop extract prepared by s...

AI Technical Summary

Problems solved by technology

The main difference of the second patent of this patent is: (1) this patent carries out certain impurity removal before loading column, removes part impurity by centrifugation, through organic filter membrane etc.; (2) CN101492357 patent adopts macroporous adsorption resin , but the effluent did not pass some qualitative means such as full-wavelength scanning and TLC analysis, resulting in low resolution, more impurities, and low xanthohumol and total flavonoid content, which cannot meet the needs of industries such as medicine and health products