The invention relates to a method for extracting and separating
xanthohumol and flavone compounds. The method comprises the following steps of: leaching hops and a supercritical CO2 extraction residue thereof with an
alcohol solution to ensure that flavone compounds, particularly
xanthohumol, are sufficiently dissolved out; filtering to obtain a filter liquor after leaching, and decompressing anddistilling the filter liquor to obtain a concentrated liquor; centrifuging the concentrated liquor, taking an obtained supernate, and filtering by using a 0.45muL of
organic film; removing partial impurities, and eluting with an eluant to elute the flavone compounds from macroporous adsorption resin; collecting
effluent liquid and concentrating; and freezing and
drying to obtain yellow
powder. In the invention, the hops, the supercritical CO2 extraction residue, and the like are used as raw materials, particularly, a
waste material which is the supercritical CO2 hop extract extraction residuein a hop
product processing process is used as the
raw material, thus the
xanthohumol which has the main
efficacy and is difficult to separate is independently extracted, and other flavone compounds rich in the hop
waste material are also extracted; and the invention also provides a development approach for hops which cannot be used for beer
brewing, thereby improving the
resource utilization ratio and the added value of the hops and having important significance to prolonging the hop industrial chain.