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Method for producing fuel ethanol by fermenting racahout

A technology of fuel ethanol and acorn powder, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of complex refining process and high cost, improve economy, simplify production process, and save fossils fuel effect

Active Publication Date: 2014-08-20
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Starch prepared from acorns can replace corn starch and other grain starches to save food, but because acorns contain a lot of tannins, the refining process is complicated and the cost is high

Method used

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  • Method for producing fuel ethanol by fermenting racahout
  • Method for producing fuel ethanol by fermenting racahout
  • Method for producing fuel ethanol by fermenting racahout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Prepare 500mL LYPD medium, the medium components are: glucose 2%, peptone 2%, yeast extract 1%. Sterilized for later use. Add 1-2 loops of bacteria sludge from FB-E slant seeds to sterilized 500mL YPD medium, and cultivate overnight at 32°C to the end of logarithmic phase to obtain activated FB-E yeast seeds.

Embodiment 2

[0023] Add 2.5L tap water, 0.6mL high-temperature amylase (40000U / mL) to 1kg acorn powder, liquefy at 100°C for 1 hour, cool down to 50°C, add 4g starch glucoamylase (30000U / g), 1g commercial cellulase (200U / g ), incubate for 14h, measure the glucose concentration as 125g / L, add 500mL nutrient solution (5% yeast extract), 200mL Saccharomyces cerevisiae FE-B seeds, 32 ℃, 500rpm, pH 5.0 fermentation, see figure 1 . From figure 1 It can be seen that the ethanol concentration basically reached the maximum after 24 hours of fermentation, and the mass concentration of ethanol at 48 hours was 7.88%, which was only 5.8% higher than the mass concentration of 7.45% at 24 hours. The mass yield of ethanol in 24h fermentation is 23.8%, and the mass yield of ethanol in 48h is 25.2%, which is equivalent to producing 1 ton of ethanol from 4 tons of acorn powder.

Embodiment 3

[0025] Add 2.5 tons of tap water to 0.8 tons of acorn powder, 25kg of yeast extract, 0.6L of high-temperature amylase (40000U / mL), liquefy at 100°C for 1 hour, cool down to 50°C, add 4kg of starch glucoamylase (30000U / g), and 1kg of commercial cellulose Enzyme (200U / g), incubated for 20h, 500L of Saccharomyces cerevisiae FE-B seeds, fermented at 32°C, 200rpm, pH 5.0. After 24 hours of fermentation, the ethanol concentration basically reached the maximum value, and the ethanol mass concentration was 8.2%.

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Abstract

The invention discloses a method for producing fuel ethanol by fermenting racahout, comprising the following steps of: firstly, saccharifying the racahout; and then, directly fermenting the saccharifed racahout by using tannin-resistant microzyme seeds, wherein the racahout saccharifying process comprises two steps of: liquefying high-temperature amylase and saccharifying starch and cellulose in the racahout by maltogenic amylase and cellulose. Compared with the prior art, the method uses non-food racahout as a raw material, directly uses tannin-resistant ethanol fermentation yeast to ferment and produce the fuel ethanol, does not adopt complicated refining processes of separating tannin, and the like, simplifies the production process of the fuel ethanol, reduces production cost and increases the economy of the production process of the fuel ethanol.

Description

technical field [0001] The invention relates to a method for processing and utilizing non-grain starch resources, in particular to a method for producing fuel ethanol by fermenting acorn powder as a raw material. Background technique [0002] How to solve the shortage of oil resources is one of the problems faced by the world in the 21st century. According to predictions, according to the current proven world oil reserves and extraction speed, the global oil supply can only last for about 40 years. The development of renewable energy, especially bio-energy is an important way to solve the energy crisis. [0003] Fuel ethanol has a high octane number and good anti-riot performance. It has become the most widely used biofuel and is an ideal substitute for gasoline. It has been widely used in some countries and regions. In 2004, 32 million tons of corn were used to produce ethanol in the United States, accounting for 11% of production. Brazil mainly uses sugarcane as raw mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C10L1/02C12R1/865
CPCY02E50/17Y02E50/10
Inventor 佟明友张全黎元生李风华
Owner CHINA PETROLEUM & CHEM CORP
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