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Impaired wound healing compositions and treatments

A composition and subject technology, applied in the field of gap junctions, can solve problems such as incomplete healing, and achieve the effect of promoting wound re-epithelialization

Inactive Publication Date: 2011-06-22
CODA THERAPEUTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Despite progress in understanding the principles underlying the wound healing process, there remains a significant unmet need for suitable therapeutic options for wound care, including wounds that do not heal at the expected rate, such as delayed healing wounds, incompletely healing wounds, and chronic wounds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0414] Peptide affinity binding assays

[0415]Pull-down assays can be used to confirm protein-peptide interactions. In this assay, test gap junction modulators (e.g., peptides) are labeled with protein activity or fusion tags, i.e., GST (glutathione-S-transferase), which are detected by attachment to cellulose, agarose, or nickel beads. Instead, they are used to capture and 'sediment' protein-binding partners. After elution of complexes with SDS-PAGE loading buffer or alternatively competitive analyte elution, complexes were visualized by electrophoresis on SDS-PAGE gels and detected using protein (Western) analysis. Methods known in the art for performing binding assays are described in Einarson, M.B. and Orlinick, J.R., "Identification of Protein-Protein Interactions with Glutathione S-Transferase Fusion Proteins" in Protein-Protein Interactions: A Molecular Cloning Manual, Cold Spring Harbor Laboratory Press, pp.37-57 (2002); Einarson, M.B., "Detection of Protein-Protein...

Embodiment 2

[0419] Functional Analysis

[0420] Functional assays can be used to determine whether a test gap junction modulating peptidomimetic is capable of blocking gap junction or hemichannel opening. HeLa human cervical cancer cell lines were stably transfected with Cx43, Cx45, or another specific connexin of interest. Cells were incubated in zero calcium solution (HBSS-HEPES, containing 1 mM EGTA), which has been shown to activate connexin hemichannels (see Braet, K., et al., "Pharmacological sensitivity of ATP release triggered by photoliberation of inositol -1, 4, 5-trisphosphate and zero extracellular calcium in brainendothelial cells," Journal of Cellular Physiology, 197(2): p.205-213 (2003); DeVries, S.H. and E.A. Schwartz, "Hemi-gap-junction channels in solitary horizontal cells of the catfish retina," Journal of Physiology 445: p.201-230 (1992); and Li, H., et al., "Properties and regulation of gap junctional hemichannels in the plasma membranes of cultured cells, "Journal ...

Embodiment 3

[0422] Peptoid Design and Testing

[0423] The peptidomimetic can be designed to have the same amino acid sequence as the extracellular loop region of connexin 43, which is thought to be involved in the linker docking (docking) process (Foote et al., J Cell Biol 140(5 ): 1187-97, (1998)). Some peptides include amino acids that match the exterior of the alpha-helical transmembrane subunit, which may show enhanced functional inhibition. Due to the conservation of connexin sequences in the extracellular loop region, not all of these peptides need be connexin-specific.

[0424] Peptides or variants thereof can be synthesized in vitro, for example, by solid phase peptide synthesis methods, as in Stewart et al., Solid Phase Peptide Synthesis, W.H. Freeman Co., San Francisco (1969); Merrifield, J. Am. Chem. Soc .852149 (1963); Meienhofer in "Hormonal Proteins and Peptides," ed.; C.H.Li, Vol.2 (Academic Press, 1973), pp.48-267; and Bavaay and Merrifield, "The Peptides," eds.E as de...

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Abstract

Methods, compounds, compositions, kits and articles of manufacture comprising one or more gap junction modulating agents for treatment of wounds that do not heal at expected rates, including chronic wounds, delayed healing wounds, incompletely healing wounds, and dehiscent wounds in a subject in need thereof.

Description

technical field [0001] The present invention relates to gap junctions and to wounds and wound healing, particularly wounds that do not heal at an expected rate, such as delayed healing wounds, incompletely healing wounds, chronic wounds, and dehiscing wounds. Background technique [0002] The following includes information that may be useful in understanding the present invention. It is not an admission that any information presented herein is prior art or relevant to the presently described or claimed invention, or that any publication or document mentioned, either explicitly or by implication, is prior art. [0003] In humans and other mammals, wound injury triggers a complex cascade of cellular and biochemical events that in most cases leads to a healing wound. An ideally healed wound is one that restores normal anatomy, function, and appearance at the cellular, tissue, organ, and organismal level. Wound healing, whether initiated by trauma, microorganisms or foreign bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K2/00A61K38/02
CPCC07K14/78A61K39/00A61K38/39A61K45/00A61P17/02A61P43/00
Inventor 戴维·L·贝克尔科林·R·格林布拉德福德·J·杜夫特
Owner CODA THERAPEUTICS INC
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