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Mixture fertilizer and preparation method thereof

A technology for microbial fertilizer and culture, applied in the agricultural field, can solve the problems of limited application method, single function, unsatisfactory effect of increasing crop yield, etc., and achieves prevention of soil-borne diseases, high biological safety, and inhibition of the reproduction of harmful bacteria. Effect

Inactive Publication Date: 2011-07-06
夏学德 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although some composite microbial agents have appeared on the market, they either have a single function, or their application methods are limited, and the crop yield increase effect after use is still unsatisfactory, so it is still necessary to develop a variety of application methods. , Composite microbial agents or fertilizers with comprehensive functions and good production-increasing effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, prepare bacterial classification fermented liquid as follows:

[0030] a. Preparation of Bacillus cereus and Bacillus subtilis fermentation broth: insert the seed liquid of Bacillus cereus and Bacillus subtilis strains into the sterilized medium at a ratio of 1:1, and the medium includes a weight ratio of 5% bean sprouts juice, 0.5% glucose, 1% peptone, the balance is water as a solvent, the inoculum size is 0.3%, aerobic fermentation is carried out at 32°C and PH is 7.2 for 24 hours, put in a tank, and the wax is obtained Bacillus-like and Bacillus subtilis fermentation broth;

[0031] b. Preparation of Pantoea agglomerans fermentation broth: insert the Pantoea agglomerans seed liquid into the sterilized culture medium, which includes 2% soybean flour, 1% corn flour, and 0.5% yeast extract by weight , 1% white sugar, 0.1% monosodium glutamate, 0.2% potassium dihydrogen phosphate, 1% calcium carbonate and 0.5% sodium chloride, the remainder is water as a ...

Embodiment 2

[0033] Embodiment 2, prepare bacterial classification fermented liquid as follows:

[0034] a, preparation of Bacillus cereus and Bacillus subtilis fermented liquid: insert Bacillus cereus BS8641, Bacillus cereus AS2015 and Bacillus subtilis K-M (Bacillus subtilis) seed liquid into the sterilized medium, medium It includes 8% bean sprouts juice, 0.3% glucose and 1.05% peptone by weight, the balance is water as a solvent, and the inoculum is 0.3%. After 24 hours of aerobic fermentation at 34°C and pH7.2 , put in a tank to obtain the mixed fermentation liquid of bacillus cereus bacillus subtilis respectively;

[0035] b. Prepare Pantoea agglomerans fermentation broth: insert the Pantoea agglomerans WLL (Pantoea agglomerans) seed liquid into the sterilized medium, the medium includes 2% soybean flour, 1.5% corn flour, 0.3% yeast extract, 2% white sugar, 0.2% monosodium glutamate, 0.2% potassium dihydrogen phosphate, 2% calcium carbonate, 0.4% sodium chloride, the balance is wate...

Embodiment 3

[0037] Embodiment 3, the fermentation broth of Bacillus cereus, Bacillus subtilis, Pantoea agglomerans, Lactobacillus acidophilus and Lactobacillus plantarum prepared in Example 1 were mixed according to the following weight ratio:

[0038] Bacillus cereus BS8641, Bacillus cereus AS2015 and Bacillus subtilis K-M accounted for 20%, Pantoea agglomerans WLL accounted for 20%, Lactobacillus plantarum and Lactobacillus acidophilus accounted for 40%, and then packed to make the microorganism of the present invention fertilizer.

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PUM

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Abstract

The invention discloses a microbial fertilizer, comprising 10-30 percent of Bacillus cereus culture, 10-30 percent of Bacillus subtilis culture, 10-30 percent of Pantoea agglomerans culture, 10-30 percent of Lactobacillus plantarum culture and 10-30 percent of Lactobacillus acidophilus culture. The microbial fertilizer can be used for soaking seeds, irrigating roots and spraying or can be used for fermenting livestock manure and a pulverized straw mixture. The microbial fertilizer also can be prepared by mixing the liquid inoculants with a well-known carrier and then drying to form the solid microbial fertilizer which is convenient to store and transport. The carrier can be bran, crop straw or other carriers suitable to be used as fertilizers.

Description

technical field [0001] The invention relates to a microbial fertilizer and a preparation method thereof, belonging to the technical field of agriculture. Background technique [0002] In order to solve or slow down the pollution caused by the use of chemical fertilizers, many countries in the world have developed biological fertilizers. Microbial fertilizers can use the vitality of microorganisms and their metabolites to improve crop nutrient supply and provide crops with nutrients and growth substances. Regulate its growth to achieve the purpose of increasing yield and quality, reducing the use of chemical fertilizers, improving soil fertility, reducing the occurrence of diseases and insect pests, and improving environmental quality. [0003] Although some composite microbial agents have appeared on the market, they either have a single function, or their application methods are limited, and the crop yield increase effect after use is still unsatisfactory, so it is still ne...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCY02W30/40
Inventor 夏学德袁杰利孟祥宜曲嫣红
Owner 夏学德
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