Method for culturing aseptic seedlings of plants
A cultivation method and technology of sterile seedlings, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as adverse effects on seed germination, interference with plant growth, and difficult washing and removal, so as to improve seed germination rate, Good growth of stems and leaves, and the effect of avoiding contamination by miscellaneous bacteria
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0030] To solve the above technical problems, the specific implementation method adopted by the present invention is:
[0031] a. Preparation of the nutrient matrix for seed germination and seedling growth: using agar powder for tissue culture, adding MS (MS is the abbreviation of the inventors of the MS medium, Murashige and Skoog, the same below) macronutrient mother liquid, MS micronutrient mother liquid and MS iron salt mother liquor, adjust pH to 5.8, autoclave: 121°C, 20 minutes, and use nutrient agar after solidification;
[0032] b. Plant seed surface disinfection method: plant seeds are placed in a microcentrifuge tube, disinfected with 75%, 95% ethanol solution and absolute ethanol successively, supplemented with sterile water for cleaning, and then sown on the nutrient substrate;
[0033] -3-
[0034] c. Germination of plant seeds: The nutrient substrate for seed germination is prepared and packed into sterile wide-mouth bottles (specification: 250mL), 20-25mL per bottle, a...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap