Method for culturing aseptic seedlings of plants

A cultivation method and technology of sterile seedlings, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as adverse effects on seed germination, interference with plant growth, and difficult washing and removal, so as to improve seed germination rate, Good growth of stems and leaves, and the effect of avoiding contamination by miscellaneous bacteria

Inactive Publication Date: 2011-07-13
SOUTHEAST UNIV
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Problems solved by technology

If using a strong disinfectant such as 0.1% HgCl 2 , or 2% sodium hypochlorite solution as reported in the literature, the disinfectant will penetrate through the seed coat, and it is not easy to wash and remove. The residual disinfectant will have a negative impact on seed germin

Method used

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  • Method for culturing aseptic seedlings of plants
  • Method for culturing aseptic seedlings of plants

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[0030] To solve the above technical problems, the specific implementation method adopted by the present invention is:

[0031] a. Preparation of the nutrient matrix for seed germination and seedling growth: using agar powder for tissue culture, adding MS (MS is the abbreviation of the inventors of the MS medium, Murashige and Skoog, the same below) macronutrient mother liquid, MS micronutrient mother liquid and MS iron salt mother liquor, adjust pH to 5.8, autoclave: 121°C, 20 minutes, and use nutrient agar after solidification;

[0032] b. Plant seed surface disinfection method: plant seeds are placed in a microcentrifuge tube, disinfected with 75%, 95% ethanol solution and absolute ethanol successively, supplemented with sterile water for cleaning, and then sown on the nutrient substrate;

[0033] -3-

[0034] c. Germination of plant seeds: The nutrient substrate for seed germination is prepared and packed into sterile wide-mouth bottles (specification: 250mL), 20-25mL per bottle, a...

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Abstract

The invention relates to a technology for culturing aseptic seedlings of plants and application thereof, particularly suitable for seedlet plants such as model plant arabidopsis thaliana, medicinal plant artemisia annua, fruit and vegetable tomato, pepper and the like. The method gets rid of the disturbances of other microbes in the environment and is particularly suitable for searching mutual action between the plants and the microbe. The method comprises steps of: preparing nutritious substrates for seed germination and seedling growth, sterilizing the surfaces of the plant seeds, and germinating the plant seeds. The method is a convenient and efficient g method for culturing aseptic seedlings of plants and has low cost; in addition, the method is free of sterilizing agent residual, thereby ensuring the reliability and accuracy of subsequent research results.

Description

technical field [0001] The invention relates to a plant aseptic seedling cultivation technology and its application. Especially suitable for small seed plants, such as model plants Arabidopsis, medicinal plants Artemisia annua, fruits and vegetables tomatoes, peppers, etc. Because it excludes the interference of other microorganisms in the environment, it is especially suitable for studying the interaction between plants and microorganisms. Background technique [0002] Since the 1970s and 1980s, with the rise of molecular biology, biologists began to look for a model plant suitable for fine research at the level of genetics and molecular biology, the flowering plant Arabidopsis thaliana ( Arabidopsis thaliana) has been found to be well suited for studies in plant genetics, developmental biology, and molecular biology. In recent years, many important discoveries in the field of plant-related research, such as root growth, flowering, hormone regulation, and plant response m...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林岚
Owner SOUTHEAST UNIV
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