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Rapid detection card of difenoconazole and detection method thereof

A technology of difenoconazole and detection card is applied in the directions of measuring devices, instruments, scientific instruments, etc., which can solve the problems of high requirements for instruments and equipment, complicated operation, etc., and achieves the effects of high sensitivity, accurate results and easy preparation.

Inactive Publication Date: 2011-08-10
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a detection device capable of detecting difenoconazole, which can directly detect the residual amount of difenoconazole in agricultural products such as vegetables and fruits by using an immunological method, and overcome the cumbersome operation of the prior art and the inconvenience to instruments and equipment. The disadvantage of high requirements, the establishment of a detection method based on immunological specificity

Method used

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  • Rapid detection card of difenoconazole and detection method thereof
  • Rapid detection card of difenoconazole and detection method thereof
  • Rapid detection card of difenoconazole and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] A difenoconazole rapid detection card, which is to set a test strip 2 in the detection card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 70mm long, 20mm wide, 5mm thick, and the thin shell wall thickness is 1mm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0022] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 60mm, the width of the strip is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyvinyl chloride plastic sheet with a ...

Embodiment 2

[0025] The processing method of the sample is as follows: take 1 gram of pulverized solid sample or liquid sample, add it into a test tube containing 3 milliliters of sample extract, shake and mix for 10 minutes. After standing for 5 minutes, gently suck 350 μL of supernatant, add an equal volume of sample diluent, mix and use for determination.

[0026] When using the difenoconazole rapid detection card for detection, the sample solution to be detected is dripped from the sample injection hole 4 onto the sample pad 8 of the difenoconazole rapid detection card. The detected sample solution and the anti-difenoconazole monoclonal antibody or polyclonal antibody colloidal gold marker contained in the colloidal gold membrane 7 diffuse to the nitrocellulose membrane 6 together, and the results are observed within 5-10 minutes.

[0027] The main reaction of the test card is the immunological antigen and antibody reaction, the colloidal gold-labeled antibody migrating on the nitrocel...

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Abstract

The invention relates to a rapid detection card of difenoconazole and a detection method thereof, which belong to the technical field of pesticide residue detection. A rapid detection card casing is internally provided with a test strip; the test strip is formed by sequentially attaching a sample pad, a colloidal gold membrane, a nitrocellulose membrane and a water sucking membrane to a bearing back panel; the colloidal gold membrane is a glass fiber membrane containing an anti-difenoconazole colloidal gold labeled antibody; and the nitrocellulose membrane is provided with two developing bands, wherein one is a detection band containing a difenoconazole protein conjugate, and the other is a quality control band containing an anti-rabbit antibody or an anti-mouse antibody. The invention has the advantages that: the residual quantity of the difenoconazole in a vegetable or fruit sample can be directly detected by an immunological method, and the rapid detection card of the difenoconazole is easy to prepare, is convenient and rapid to use, and ensures accurate detection result.

Description

technical field [0001] The invention relates to a difenoconazole rapid detection card and a detection method thereof, is a detection device for detecting difenoconazole, and belongs to the technical field of pesticide residue detection. Background technique [0002] Difenoconazole belongs to a class of insecticides containing triazoles. It has protective, therapeutic and systemic activities. It is a sterol demethylation inhibitor, inhibits the biosynthesis of cell wall sterols, and prevents the growth of fungi. Its bactericidal spectrum is wide, for Ascomycotina, Basidiomycotina and including Alternaria, Ascodiosporum, Cercospora, Cercospora, Globula, Phoma, Cylindrica Some semi-known fungi including Septoria, Scutellaria, Powdery mildewaceae, and Rust fungi have long-lasting protection and therapeutic activity. Leaf blight, rust and mildew caused by several pathogens, apple scab, powdery mildew, grape powdery mildew, potato early blight, peanut leaf spot, net spot, etc. ha...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 周坚吴文晔熊晓芳
Owner 无锡安迪生物工程有限公司