Fungus bactericide for degrading organophosphorus insecticide with broad spectrum

A broad-spectrum, strain-based technology, applied in the field of microorganisms, to achieve good soil remediation effects, rich degradation enzymes, and stable genetic traits

Active Publication Date: 2011-08-17
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of two or more fungi to jointly degrade organophosphorus pesticide residues

Method used

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  • Fungus bactericide for degrading organophosphorus insecticide with broad spectrum
  • Fungus bactericide for degrading organophosphorus insecticide with broad spectrum
  • Fungus bactericide for degrading organophosphorus insecticide with broad spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Take Aspergillus niger J4 and J6 slant strains, inoculate them on PDA medium (peeled potato 200g, sucrose 20g, agar 15-20g, natural pH) plate, culture at 25-32°C for 5 days, and use sterilized Glucose basal salt medium (NaCl0.1%, KH 2 PO 4 0.05%, K 2 HPO 4 0.15%, NH 4 NO 3 0.1%, MgSO 4 ·7H 2 O 0.01%, glucose 0.1%, pH5.5-7.5) to wash the plate, respectively prepared to a density of about 3 × 10 8 Each / ml suspension was added to glucose basal salt medium (NaCl 0.1%, KH 2 PO 4 0.05%, K 2 HPO 4 0.15%, NH 4 NO 3 0.1%, MgSO 4 ·7H 2 (0.01%, glucose 0.1%, pH 5.5-7.5) at 30 ° C, after 6 hours, the spores germinate into spore seed liquid. The spore seed liquid of Aspergillus niger J4 and J6 is prepared into fungal inoculum with a volume ratio of 0.5:1, and the inoculum is inoculated in 100ml with phorate as the only carbon source (containing phorate 300mg / L) by 6% inoculation amount In the basal salt culture medium, at the same time with no inoculation as the bla...

Embodiment 2

[0050] In the same way as in Example 1, the spore seed liquid of Aspergillus niger J4 and J6 is prepared with a volume ratio of 0.5:1 to obtain the fungal inoculant, and the 100mg / L phorate aqueous solution is evenly sprayed on 200g of the sterilization test soil, and the Inoculate 20ml fungal agent into the test soil and mix evenly, add sterile water to adjust the water content to 60% soil humidity, place the test soil at 30°C for cultivation, and use the non-inoculated as the blank control, every Samples were taken on the first day to determine the residual amount of phorate by gas chromatography, and sterile water was added during the cultivation process to maintain 60% soil humidity. After culturing for 5 days, the degradation rate of phorate was measured to be 82%.

Embodiment 3

[0052] In the same way as in Example 1, the spore seed liquid of Aspergillus niger J4 and J6 is prepared with a volume ratio of 3:1 to obtain the fungal inoculant, and the 1000mg / L omethoate aqueous solution is evenly sprayed on the 200g sterilization test soil, and the Inoculate 20ml fungal agent into the test soil and mix evenly, add sterile water to adjust the water content to 60% soil humidity, place the test soil at 30°C for cultivation, and use the non-inoculated as the blank control, every Samples were taken one day to determine the residual amount of phorate by gas chromatography, and sterile water was added during the cultivation process to maintain a soil humidity of 60%. After cultivating for 5 days, the degradation rate of omethoate was measured to be 81%.

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Abstract

The invention belongs to the technical field of microbe, and in particular relates to a fungus bactericide for degrading organophosphorus insecticide with broad spectrum. The preparation method of the fungus bactericide comprises the following steps of: respectively vaccinating aspergillus niger J4 and aspergillus niger J6 to an inclined surface of a physical design agent (PDA) to form inclined-surface strains; cultivating till that spore is mature by a PDA culture medium, and respectively adding the matured spore into a glucose basic salt culture medium; cultivating till that the spore is sprouted to form spore seed liquid; and mixing two seed liquids according to the volume ratio of (0.1-3): 1. The preservation number of the vaccinating aspergillus niger J4 in the china general microbiological culture collection center is China general microbiological culture collection (CGMCC) NO. 4219, and the preservation number of the vaccinating aspergillus niger J6 in the china general microbiological culture collection center is CGMCC NO. 4220. The fungus bactericide can be used for effectively degrading organophosphorus insecticide such as thiophosphates (P=S) and phosphates (P=O), and is abundant in degrading enzyme system, wide in application range, high in degrading ratio, and short in degrading time.

Description

1. Technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a technology for degrading pesticide residues by microorganisms, in particular to a fungal agent for broad-spectrum degradation of soil organophosphorus pesticide residues. 2. Technical Background [0002] my country is a big country in the production and use of pesticides. The annual pesticide usage is about 800,000 tons, ranking first in the world. The total amount of pesticide production has been ranked second in the world since 1990, second only to the United States. At present, my country's pesticide product structure is still dominated by insecticides, and the production of insecticides accounts for about 70% of the total output of pesticides, of which organophosphorus insecticides account for 70% of the total output of pesticides, with an annual output of more than 10,000 tons Of the 6 insecticide varieties in China, 5 are organophosphate insecticides. Organophosph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10A62D101/28C12R1/685C12N1/14A62D101/04A62D3/02
Inventor 谢宝恩王继雯甄静周伏忠陈国参李冠杰刘莹莹
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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