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Actinomucor elegans ZGB1 and application thereof in microbial ATP (Adenosine Triphosphate) synthesis
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A technology of Mucor radiograss and microorganisms, applied to Mucor radiotae ZGB1 and its application in ATP synthesis by microorganisms
Active Publication Date: 2012-05-23
ZHEJIANG UNIV OF TECH
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The publicly reported adopting adenine as the method for the microbial transformation and synthesis of ATP of the precursor raw material mainly contains: Japan Kyowa Fermentation Co., Ltd. adopts ammonia-producing bacteria (Cornebacterium ammoniagenes) to transform and synthesize ATP (Biosci.Boitechnol.Biochem., 65 (3), 644 ~650, 2001); Domestic Sun Yat-sen University used brewer’s yeast to transform and synthesize ATP (Journal of Sun Yat-sen University, Natural Science Edition, 3, 15-26, 1975); Zhan Guyu et al. transformed and synthesized 7 strains of Mucor aromaticus (Mucor aromaticus) ATP (Natural Science Edition, Journal of Northwest University, 02, 118-129, 1980), etc., so far there is no document or patent report on using adenine as a precursor material to transform and synthesize ATP by Mucoralus elegans species
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Embodiment 1
[0040] Inoculated into PDA slant medium, cultured at 28°C for 3-4 days, washed with physiological saline to make spore suspension (about 10 6 ~10 7 cfu / mL);
[0041] The spore suspension was inserted into the liquid seed culture medium (glucose 20g / L, peptone 10g / L, yeast extract 5g / L, NaCl 5g / L, solvent is water, pH 6.0~6.5) with 10% (V / V) inoculum size ), placed in a 28°C, 160r / min rotary shaker for 12h to obtain a liquid seed solution;
[0042] The liquid seed solution is inserted into the fermentation medium (glucose 40g / L, yeast extract 3g / L, corn steep liquor 10g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 O 0.2g / L, NH 4 Cl 3g / L, K 2 HPO 4 ·3H 2 (O 3g / L, solvent is water, pH 6.0~6.5), placed in 28°C, 160r / min rotary shaker for 12h, and the fermentation ended.
[0043] The fermentation broth was collected by suction filtration, rinsed and dried with sterile water, and then dried in a constant temperature drying oven at 65° C. for 3 hours for cell permeabilization treat...
Embodiment 2
[0047] Mucor actinosa ZGB1 (CGMCC No.4313) solid slant medium (glucose 20g / L, peptone 10g / L, yeast extract 5g / L, NaCl 5g / L, agar 20g / L, solvent is water, pH 6.0~6.5 ), cultured at 28°C for 3-4 days, washed with normal saline to make spore suspension (about 10 6 ~10 7 cfu / mL);
[0048] The spore suspension was inserted into the liquid seed culture medium (glucose 20g / L, peptone 10g / L, yeast extract 5g / L, NaCl 5g / L, solvent is water, pH 6.0~6.5) with 10% (V / V) inoculum size ), placed in a 28°C, 160r / min rotary shaker for 12h to obtain a liquid seed solution;
[0049] The liquid seed solution is inserted into the fermentation medium (glucose 40g / L, yeast extract 3g / L, corn steep liquor 10g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 O 0.2g / L, NH 4 Cl 3g / L, K 2 HPO 4 ·3H 2 (O 3g / L, solvent is water, pH 6.0~6.5), placed in 28°C, 160r / min rotary shaker for 12h, and the fermentation ended.
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Abstract
The invention provides a novel strain capable of synthesizing ATP (Adenosine Triphosphate), namely an Actinomucor elegans ZGB1 and application thereof. The strain is preserved in China General Microbiological Culture Collection Center (address: Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Precinct, Beichen West Road, Chaoyang District, Beijing; zip code: 100101), the preservation number is CGMCC No.4313, and the preservation date is November 8th, 2010. By using the strain, adenine can be used as the precursor for ATP synthesis, thereby achieving the advantages of low material cost, abundant material source, simple formula of thallus culture medium, easy thallusseparation process and the like; and the conversion efficiency is high: ATP of higher than 10g / L can be produced in the reaction solution containing adenine of higher than 3g / L.
Description
(1) Technical field [0001] The invention relates to a new bacterial strain capable of synthesizing ATP--Actinomucor elegans ZGB1, and its application in microbial synthesis of ATP. (2) Background technology [0002] ATP is adenosine triphosphate. As a biochemical drug, it has been used in the treatment or adjuvant treatment of muscleatrophy, myocardial infarction, hepatitis, etc. and various emergency diseases. [0003] At present, ATP is mainly produced by microbial transformation at home and abroad, specifically, there are two main production methods using adenosine and adenine as precursor raw materials. The publicly reported adopting adenine as the method for the microbial transformation and synthesis of ATP of the precursor raw material mainly contains: Japan Kyowa Fermentation Co., Ltd. adopts ammonia-producing bacteria (Cornebacterium ammoniagenes) to transform and synthesize ATP (Biosci.Boitechnol.Biochem., 65 (3), 644 ~650, 2001); Domestic Sun Yat-sen University u...
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