Detection of phosphatidylcholine in yolk lecithin by using high performance liquid chromatography

Abstract

The invention provides a method for detecting phosphatidylcholine, which is used for determining the chemical composition and quantifying the phosphatidylcholine in yolk lecithin by using a method of using both high performance liquid chromatography and an ultraviolet detector. The method is characterized in that a silicagel column ZORBAX RX-SIL (4.6 mm X 250 mm, 5 microns) manufactured by Agilent is adopted as an analytical column and methanol / acetic acid is used as a mobile phase to carry out isocratic elution, and other elution conditions are an ultraviolet detection wavelength of 200 nm, a column temperature of 30 DEG C, a sample feeding quantity of 15 microliters and a flowing speed of 0.5 ml / min. The mobile phase of the method has the characteristics of binary system, simpler composition, lower flowing speed of 0.5 ml / min, faster peak time within 14 minutes and linear range of a phosphatidylcholine standard curve of 10-160 microgram / ml; the added 3.75% acetic acid solution improves a peak shape of the phosphatidylcholine and effectively inhibits serious tailing phenomenon; and the use cost of a solvent is saved. It is proved by a methodological replication experiment that the method has the advantages of high precision, good stability and higher accuracy.

Description

Technical field [0001] The method belongs to the field of functional factor detection methods, and relates to a method for detecting phosphatidylcholine in egg yolk lecithin by adopting high performance liquid chromatography. Background technique [0002] Lecithin is a phosphorus-containing polar lipid substance, which usually refers to a mixture of different phospholipid components such as phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Lecithin has important nutritional and health care functions. It is an important component of biofilm. It has the functions of delaying aging, regulating fat metabolism, preventing cardiovascular and cerebrovascular diseases, improving brain vitality, and improving immunity. It is widely used in food, medicine, cosmetics, feed, paint, agricultural production and other fields . Phosphatidylcholine is the most important functional component in lecithin. At present, there are many researches on soybean lecithin. In recent...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: to make up for the complex composition of the mobile phase system in the existing liquid phase analysis method of phosphatidylcholine, the relatively large flow rate, and the relatively large amount of standard substance required when using an ultraviolet detector. The present invention provides Qualitative and quantitative determination of phosphatidylcholine in egg yolk lecithin by a high-performance liquid chromatography coupled with ultraviolet detector

The linear range of phosphatidylcholine standard curve is 10μg / ml~160μg / ml

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