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A spray for treating diseases caused by human papilloma viruses and a preparation method

A technology of human papillomavirus and virus infection, which is applied in the field of medicine to achieve the effect of phagocytosis activation and phagocytosis enhancement

Active Publication Date: 2011-08-31
孙介光 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the external dosage form and preparation method of inhibiting human papillomavirus and treating tumors caused by human papillomavirus infection and using purified human rabies vaccine to treat sexually transmitted diseases have not yet been reported.

Method used

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  • A spray for treating diseases caused by human papilloma viruses and a preparation method
  • A spray for treating diseases caused by human papilloma viruses and a preparation method
  • A spray for treating diseases caused by human papilloma viruses and a preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Ingredients of the spray of the present invention:

[0031] According to the volume ratio, a purified human rabies vaccine with a composition of 1ml is diluted to 10ml with a mass volume concentration of 0.01g / ml chitosan-acetic acid solution.

[0032] The specific equipment method is as follows:

[0033] 1. Prepare chitosan-acetic acid solution (pH=3.2)

[0034] (1) Prepare a 0.3% acetic acid solution: add 3ml of glacial acetic acid (analytical grade) to a 1000ml volumetric flask, add pure water to the mark, where the pure water is double distilled water to obtain an acetic acid solution with a volume percentage of 0.3% ;

[0035] (2) Prepare a chitosan-acetic acid solution with a mass volume concentration of 0.01g / ml: Weigh 10g of chitosan and add it to the above acetic acid solution to completely dissolve it to 1000ml, and the solution does not allow insoluble particles or insoluble matter to form a mass Chitosan-acetic acid solution with a volume concentration of 0.01g / ml; ...

Embodiment 2

[0041] The difference from Example 1 is:

[0042] 1. Prepare chitosan-acetic acid solution (pH=4.9):

[0043] 4ml of glacial acetic acid used to prepare 0.4% acetic acid solution is (chemically pure); the pH value is adjusted to 4.9 with a sodium hydroxide solution with a molar concentration of 0.1 mol / L or an acetic acid solution with a molar concentration of 0.1 mol / L.

[0044] Prepare a chitosan-acetic acid solution with a mass volume concentration of 0.02g / ml: weigh 20g of chitosan and add it to the above acetic acid solution to completely dissolve it to 1000ml, the solution does not allow insoluble particles or insoluble matter, and the mass volume concentration is 0.02g / ml chitosan-acetic acid solution.

[0045] 2. Preparation of vaccine solution

[0046] Take 1ml of the freeze-dried purified human rabies vaccine with an activity unit of 15IU (in this example, use two, each 0.5ml, 7.5IU) and add it to a 10ml measuring flask. The preparation of the purified human rabies vaccine is...

Embodiment 3

[0048] The difference from Example 1 is:

[0049] 1. Prepare chitosan-acetic acid solution (pH=5.0):

[0050] 10ml of glacial acetic acid used to prepare 1.0% acetic acid solution is chemically pure; the pH is adjusted to 5.0 with a sodium hydroxide solution with a molar concentration of 0.1 mol / L or an acetic acid solution with a molar concentration of 0.1 mol / L.

[0051] Prepare a chitosan-acetic acid solution with a mass volume concentration of 0.03g / ml: Weigh 30g of chitosan and add it to the above acetic acid solution to completely dissolve it to 1000ml. The solution does not allow insoluble particles or insoluble matter, and the mass volume concentration is 0.03g / ml chitosan-acetic acid solution.

[0052] 2. Preparation of vaccine solution

[0053] Take 1ml of purified human rabies vaccine with an activity unit of 10IU (two in this example, 0.5ml each, 5IU) and add the solution to a 10ml volumetric flask. The purified human rabies vaccine is prepared as rabies virus CTN. Strain....

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Abstract

The invention relates to the field of pharmaceutical technology, particularly to a spray for treating diseases caused by human papilloma viruses and a preparation method. The spray comprises, by volume ratio, 1 ml of rabies purified vaccine for human use and 9 ml of chitosan-acetic acid solution with a prepared mass volume concentration being 0.01 g / ml to 0.03 g / ml, wherein the chitosan-acetic acid solution is prepared by fully dissolving 10g to 30g chitosan and the acetic acid solution until the volume reaches 1000 ml. The preparation method comprises the steps of first preparing 0.3% to 1.0% of the acetic acid solution and then preparing the chitosan-acetic acid solution; adding 1 part of the rabies purified vaccine for human use with an active content being 5 IU to 20 IU by volume to a measuring flask with a volume of 10 parts, adding the chitosan-acetic acid solution, and diluting for preparing the vaccine solution. The spray and the preparation method thereof in the invention is focused on follow-up treatments (Tc subgroup detection, HPV detection and improvement of clinical symptoms) of cervical carcinoma patients infected by HPV, HPV-infected people, and venereal diseases, cervical erosion and chronic cervicitis caused by HPV infection, and achieves good clinical results.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to a spray for treating diseases caused by human papillomavirus infection and its preparation. Background technique [0002] Purified human rabies vaccine (KI) was found in clinical use as a biological antigen to stimulate the body, which can induce the body to produce a strong immune response (antigen-specific immunity; non-antigen-specific immunity). Immune response plays a very important role in controlling related diseases. A large number of literature studies have proved that: the human body's low immunity is closely related to the occurrence of cancer. Once the body's immunity is reduced, various diseases can take advantage of the gap. Clinical data also proves that in the research on the relationship between tumor viruses and human diseases, only human papilloma virus (HPV) has the most clear relationship with human tumors. In 1995, WHO and IARC have identified HPV as the cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/205A61K9/12A61K47/36A61P31/20A61P15/00
CPCC12N2760/20134A61K9/0014A61K2039/541A61K39/205A61K9/12A61K9/0034A61K2039/54A61K39/12A61K2039/585A61P15/00A61P31/14A61P31/20A61P35/00
Inventor 孙介光孙雪然
Owner 孙介光
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