Method and special strain for cultivating epiphytic orchid

A technology of endophytic fungi and bacterial agents, which is applied in the field of cultivating epiphytic orchids, can solve problems such as artificial cultivation techniques that have not been effectively solved, low survival rate, wild resource dependence and destructive increase, and achieve broad application prospects and seedlings. Effects of growth promotion and alleviating the scarcity of wild resources

Inactive Publication Date: 2011-09-07
HAINAN UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

As artificial cultivation techniques have not been effectively resolved, the dependence on wild resources and the resulting destructiveness are increasing day by day
The low survival rate of transplanted ti

Method used

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  • Method and special strain for cultivating epiphytic orchid
  • Method and special strain for cultivating epiphytic orchid
  • Method and special strain for cultivating epiphytic orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, isolation and identification of fungi

[0031] 1. Separation method

[0032] Isolate strains from the wild Dendrobium sinensis collected from Bawangling, Hainan Province, the method is as follows:

[0033] 1.1. Select the fresh vegetative roots of wild Dendrobium sinensis that grow vigorously and grow vigorously, and wash away the humus and sundries on the root surface with running water.

[0034] 1.2. Immerse the root segment in 1% calcium hypochlorite solution for 10-12 minutes on the ultra-bacteria workbench for surface sterilization, and then rinse it with sterile water for 3-4 times.

[0035] 1.3. Cut the root section into 2-3mm long pieces, place it on the PDA medium, and cultivate it in the dark at 25°C. After a period of cultivation, after the mycelium grows from the root section, pick the mycelium and transfer it Cultivate and purify on PDA medium.

[0036] 1.4. No pollution, transfer to PDA slant medium in finger tube, store at 4°C for short-t...

Embodiment 2

[0043] Embodiment 2, the cultivation of Dendrobium sinensis

[0044] 1. Collection and pretreatment of Dendrobium sinensis seeds

[0045] Mature and uncracked fruit pods of Dendrobium chinensis were taken.

[0046] Disinfection treatment of seeds: put well-grown, mature and uncracked fruit pods into 1.5% (mass percent) calcium hypochlorite aqueous solution for 13-15 minutes for surface sterilization, and rinse with sterile water for 3-4 times.

[0047] Second, the preparation of bacteria

[0048] 1. Activation

[0049] Pseudocercospora (Pseudocercospora) S3 CGMCC No.4148 was inoculated in PDA medium, and activated in the dark at 25°C.

[0050] Preparation of PDA medium: Same as in the prior art, every liter of PDA medium is made of 200 grams of peeled potatoes, 20 g of sucrose, 20 g of agar powder and 1 L of water, and the pH is natural.

[0051] 2. Bacterial block culture

[0052] The culture block with a single pure hyphae was inoculated in DE medium, and cultured in da...

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Abstract

The invention discloses a method and special strain for cultivating epiphytic orchid. The strain provided by the invention is pseudocercospora sp. S3CGMCC No. 4148. The strain has an obvious effect of promoting seedling growth, can be used as high-quality symbiotic bacteria for growth of dendrobium nobile and can be applied to gardening and planting production of the plant. The strain has a broad application prospect in conservation and large-scale propagation of other orchid plants, particularly epiphytic orchid plants.

Description

technical field [0001] The invention relates to a method for cultivating epiphytic orchids and special bacterial strains. Background technique [0002] Orchidaceae is a special plant group and one of the most evolved families of angiosperms. Among them, bletilla striata, gastrodia elata, dendrobium, etc. can be used as medicine; Therefore, with the increasing demand of the market, wild resources have been seriously damaged day by day. [0003] In the wild, orchid seedlings depend on the presence of the right fungus for their growth. Most of what is known about orchid mycorrhizal fungi has been obtained from laboratory studies of fungi isolated from the roots of adult orchid strains. The extremely small size of orchid seeds makes it impossible to track and investigate the underground life stages of orchids from seed germination to plant emergence, so little is known about the temporal dynamics of orchid seed germination. Rasmussen&Whigham proposed for the first time a see...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G7/00
Inventor 余文刚钟云芳朱国鹏宋希强李霖明
Owner HAINAN UNIVERSITY
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