Method for preparing premixed dry powder of plant tissue culture medium

Abstract

The invention belongs to the field of agricultural biotechnology, particularly relating to a method for preparing premixed dry powder of a plant tissue culture medium. The method for preparing the premixed dry powder of the plant tissue culture medium comprises the following steps of: (1) preparing trace mineral elements, organic matters and iron salts into mother liquors respectively; (2) adding agar into the trace mineral element mother liquor, the organic matter mother liquor and the iron salt mother liquor respectively, boiling into solutions, cooling, then placing in a thermostat for 6 to 7 days and drying, and crushing and sieving; (3) weighing each macroelement composition, placing in the thermostat and drying, grinding, sieving and uniformly mixing; and (4) uniformly mixing the prepared powder according to a formula, and drying and storing at room temperature. The plant tissue culture medium prepared with the method has the advantages of stable property, light weight, no need of low-temperature storage, convenience in storage, transportation and use and contributes to being used by most of laboratories and tissue culture enthusiasts.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a preparation method of plant tissue culture medium premixed dry powder. Background technique [0002] The nutrient composition of the medium generally used for plant tissue culture (referred to as plant tissue culture) is basically consistent with the nutritional requirements of the whole plant. Although cell culture, organ culture, shoot tip culture or callus culture have their own special requirements, they are The medium used usually includes carbon sources, inorganic salts, vitamins, growth hormones, and trace organic components. The concentration of each nutrient, and even the relative concentration of each other has a major impact on growth, and requires extremely precise preparation. The preparation of conventional plant tissue culture medium is generally to prepare a mixed high-concentration solution (mother solution) with several substances. When us...

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Problems solved by technology

The configuration process is extremely cumbersome, and the configuration and storage of the mother liquor has the following problems: (1) Some mineral elements are easy to cause the mother liquor to precipitate; (2) The mother liquor containing organic substances is easy to be contaminated by bacteria; (3) The mother liquor needs to be stored at low temperature and transportation is inconvenient

Premixed medium configuration published in existing literature (Yihong, Wang Lili, Dong Xiuping, etc. Preparation of dehydrated plant tissue culture medium [J]. Journal of Hebei Institute of Light and Chemical Industry, 1998, 19(2):80-82.) The method is the sucrose wrapping method, which is cumbersome and time-consuming, and requires low-temperature drying or special equipment

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