Method for preparing premixed dry powder of plant tissue culture medium

A plant tissue and culture medium technology, applied in the field of agricultural biology, can solve the problems of low-temperature storage and transportation of mother liquor, cumbersome process, and long time consumption, and achieve the effects of convenient storage, transportation and use, simple preparation method, and good regeneration effect

Inactive Publication Date: 2011-09-07
HENAN UNIV OF SCI & TECH
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Problems solved by technology

The configuration process is extremely cumbersome, and the configuration and storage of the mother liquor has the following problems: (1) Some mineral elements are easy to cause the mother liquor to precipitate; (2) The mother liquor containing organic substances is easy to be contaminated by bacteria; (3) The mother liquor needs to be stored at low temperature and transportation is inc

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  • Method for preparing premixed dry powder of plant tissue culture medium

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Embodiment 1

[0021] Taking the formula of MS medium as an example, the medium of 1 L is prepared by the preparation method of medium premix dry powder of the present invention.

[0022] Table 1 MS medium formula composition list

[0023]

[0024] Concrete preparation steps are as follows:

[0025] (1) According to the formula, the trace elements, organic matter, and iron salt are prepared into 200 times concentrated liquid, that is, the mother liquid;

[0026] (2) Take the mother liquor of trace elements, add agar and an appropriate amount of water to boil to make a 3% solution, after cooling, place it in a 40°C thermostat for 7 days to dry, crush and sieve;

[0027] (3) Take the mother liquor of organic matter, add agar and appropriate amount of water to boil to make a 3% solution, after cooling, place it in a 40°C thermostat for 7 days to dry, crush and sieve;

[0028] (4) Take the iron salt mother liquor, add agar and appropriate amount of water to boil to make a 3% solution, after...

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Abstract

The invention belongs to the field of agricultural biotechnology, particularly relating to a method for preparing premixed dry powder of a plant tissue culture medium. The method for preparing the premixed dry powder of the plant tissue culture medium comprises the following steps of: (1) preparing trace mineral elements, organic matters and iron salts into mother liquors respectively; (2) adding agar into the trace mineral element mother liquor, the organic matter mother liquor and the iron salt mother liquor respectively, boiling into solutions, cooling, then placing in a thermostat for 6 to 7 days and drying, and crushing and sieving; (3) weighing each macroelement composition, placing in the thermostat and drying, grinding, sieving and uniformly mixing; and (4) uniformly mixing the prepared powder according to a formula, and drying and storing at room temperature. The plant tissue culture medium prepared with the method has the advantages of stable property, light weight, no need of low-temperature storage, convenience in storage, transportation and use and contributes to being used by most of laboratories and tissue culture enthusiasts.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a preparation method of plant tissue culture medium premixed dry powder. Background technique [0002] The nutrient composition of the medium generally used for plant tissue culture (referred to as plant tissue culture) is basically consistent with the nutritional requirements of the whole plant. Although cell culture, organ culture, shoot tip culture or callus culture have their own special requirements, they are The medium used usually includes carbon sources, inorganic salts, vitamins, growth hormones, and trace organic components. The concentration of each nutrient, and even the relative concentration of each other has a major impact on growth, and requires extremely precise preparation. The preparation of conventional plant tissue culture medium is generally to prepare a mixed high-concentration solution (mother solution) with several substances. When us...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 吴正景王少先高文陈迪新刘保国孙淑伟刘亚楠
Owner HENAN UNIV OF SCI & TECH
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