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Method for separating and purifying phycophage from blue algae outbreak water body

A technology for separation and purification, algae phage, applied in microorganism-based methods, biochemical equipment and methods, recovery/purification, etc., and can solve problems such as complex components

Inactive Publication Date: 2011-09-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the components in natural water bodies are complex and there are many kinds of microorganisms. How to efficiently separate and purify cyanophages is difficult, and there are no relevant reports so far.

Method used

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  • Method for separating and purifying phycophage from blue algae outbreak water body
  • Method for separating and purifying phycophage from blue algae outbreak water body

Examples

Experimental program
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Effect test

Embodiment 1

[0013] A method for isolating and purifying cyanophages from water bodies where cyanobacteria explodes, is characterized in that the following steps are adopted:

[0014] A) Collect water samples from the surface layer of natural waters where cyanobacteria have exploded, and store them in an ice bath. After the water samples pass through microporous membranes with a pore size of 0.80 μm, 0.45 μm, and 0.22 μm, they are concentrated into concentrated water samples by an ultrafiltration system. Its concentration factor is 200 times;

[0015] B) Prepare a mixed solution of concentrated water samples and logarithmic phase Microcystis aeruginosa liquid, and cultivate it for 7 days under the conditions of light intensity of 2000lux, temperature of 30°C, and light-dark cycle of 12h:12h to obtain cracked Microcystis aeruginosa When the color becomes transparent, it is determined that the water sample contains cyanophages; the volume ratio of the concentrated water sample and the logari...

Embodiment 2

[0019] A method for isolating and purifying cyanophages from water bodies where cyanobacteria explodes, is characterized in that the following steps are adopted:

[0020] A) Collect water samples from the surface layer of natural waters where cyanobacteria have exploded, and store them in an ice bath. After the water samples pass through microporous membranes with a pore size of 0.80 μm, 0.45 μm, and 0.22 μm, they are concentrated into concentrated water samples by an ultrafiltration system. Its concentration factor is 1000 times;

[0021] B) Prepare a mixture of concentrated water samples and logarithmic phase Microcystis aeruginosa liquid, and cultivate it for 3 days under the conditions of light intensity of 2000 lux, temperature of 30°C, and light-dark cycle of 12h:12h to obtain cracked Microcystis aeruginosa When the color becomes transparent, it is determined that the water sample contains cyanophages; the volume ratio of the concentrated water sample and the logarithmic...

Embodiment 3

[0025] A method for isolating and purifying cyanophages from water bodies where cyanobacteria explodes, is characterized in that the following steps are adopted:

[0026] A) Collect water samples from the surface layer of natural waters where cyanobacteria have exploded, and store them in an ice bath. After the water samples pass through microporous membranes with a pore size of 0.80 μm, 0.45 μm, and 0.22 μm, they are concentrated into concentrated water samples by an ultrafiltration system. Its concentration factor is 600 times;

[0027] B) Prepare a mixture of concentrated water samples and logarithmic phase Microcystis aeruginosa liquid, and cultivate it for 5 days under the conditions of light intensity of 2000lux, temperature of 30°C, and light-dark cycle of 12h:12h to obtain Microcystis aeruginosa lysate When the color becomes transparent, it is determined that the water sample contains cyanophages; the volume ratio of the concentrated water sample and the logarithmic ph...

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Abstract

The invention discloses a method for separating and purifying phycophage from a blue algae outbreak water body. The method is characterized by comprising the following steps of: A) acquiring a water sample of the surface layer of a blue algae outbreak natural water area, filtering the water sample through a micropore filter membrane, and concentrating the filtered water sample to obtain a concentrated water sample; B) preparing mixed solution of the concentrated water sample and patina microcystis liquid of a logarithmic phase, obtaining patina microcystis cracked solution by culturing, and judging that the water sample contains the phycophage when the color of the water sample becomes transparent; and C) filtering the cracked solution through the micropore filter membrane, removing patina microcystis cracked fragments, concentrating the solution by an ultra-filtration system, centrifuging and purifying the phycophage by using CsCl density gradient, putting a strip sucking viruses in the CsCl density gradient into styrene monomer (SM) buffer solution, and performing dialysis to obtain dialysis solution, namely phycophage purified solution. Compared with the prior art, the method has the advantages that: the phycophage can be successfully separated and purified from the blue algae outbreak water body; and the method does not cause environmental pollution.

Description

technical field [0001] The invention relates to a method for separating and purifying cyanophages, in particular to a method for separating and purifying cyanophages from water bodies with cyanobacteria outbreaks. Background technique [0002] Cycophage is a kind of virus that infects cyanobacteria, and its morphology is similar to that of bacteriophage. Recent studies have shown that cyanophage, as an active dynamic factor in the water environment, can play an important role in controlling the primary productivity of water and harmful algal blooms , and even affect the food chain in the water ecosystem, so how to effectively separate and purify cyanophages is of great significance for studying the physiological and ecological characteristics of cyanophages in water bodies. However, natural water has complex components and a wide variety of microorganisms. How to efficiently separate and purify cyanophages is difficult, and there are no relevant reports so far. Contents of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/02C12R1/92
Inventor 郭宗楼黄朴刘露武海涛徐立红
Owner ZHEJIANG UNIV