Method for preparing rapeseed bioactive peptide

A bioactive peptide and rapeseed technology, which is applied in the field of protease hydrolysis technology, can solve the problems of uneven molecular weight distribution of products, affecting the physiological activity of products, and high salt content in finished products.

Active Publication Date: 2011-09-07
武汉百信正源生物技术工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology of preparing rapeseed peptide by enzymatic hydrolysis, a large amount of lye or acid solution is added in the enzymatic hydrolysis process to adjust the pH of the solution during the enzymatic hydrolysis reaction, and there is no desalination treatment in the process, so that the salt content of the finished product is too high; and , the enzymatic solution contains a large amount of pigment components, which will affect the color of the finished product
In addition, due to the different enzymolysis conditions, the molecular weight distribution of the product after enzymolysis is not uniform, which affects the physiological activity of the product.

Method used

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  • Method for preparing rapeseed bioactive peptide
  • Method for preparing rapeseed bioactive peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Dissolve 100 g of defatted rapeseed meal into 800 mL of water, pulverize it with a colloid mill, adjust the pH to 10.0 with NaOH reagent, stir continuously for 15 min, 3000 rpm, 15 min, and centrifuge to take the supernatant;

[0040] (2) Heat the supernatant from step 1 to 90°C, adjust the pH value to 3.5 with citric acid, set the temperature at 45°C, and stir at a speed of 45r / min to precipitate the protein for 30min, then centrifuge at 3000r / min for 10min, and remove the supernatant liquid, collect the solid phase, add water and mix again according to the above steps, adjust the pH value to 3.5 with citric acid, and precipitate the protein, repeat three times, and finally wash twice with water, centrifuge to obtain the solid phase, which is rapeseed protein, and weigh as 58g;

[0041] (3) Pour the precipitate in step 2 into 600mL of water, heat to 90°C, keep for 30min, then cool to 50°C, add 2.32g of neutral protease As1.398 (enzyme activity is 130000U / g), adjust...

Embodiment 2

[0051] (1) Dissolve 500 g of rapeseed meal into 5 L of water, pulverize it with a colloid mill, adjust the pH to 12.0 with NaOH reagent, stir continuously for 15 min, 3000 rpm, 15 min, and centrifuge to get the supernatant;

[0052] (2) Heat the supernatant from step 1 to 90°C, adjust the pH value to 4.2 with citric acid, set the temperature at 50°C, and stir at a speed of 30r / min to precipitate the protein for 45min, then centrifuge at 3000r / min for 5min, and remove the supernatant liquid, collect the solid phase, add water and mix again according to the above steps, adjust the pH value to 4.2 with citric acid, and precipitate the protein, repeat three times, and finally wash twice with water, centrifuge to obtain the solid phase, which is rapeseed protein, and weigh as 326g;

[0053] (3) Pour the precipitate in step 2 into 4L water, heat to 95°C, keep for 30min, then cool to 45°C, add 14.67g of neutral protease As1.398 (enzyme activity is 130000U / g), adjust with NaOH reagent...

Embodiment 3

[0063] (1) Take 1000g of rapeseed meal and dissolve it in 12L of water, pulverize it with a colloid mill, adjust the pH to 9.0 with NaOH reagent, stir continuously for 15min, 3000rpm, 15min, and centrifuge to get the supernatant;

[0064] (2) Heat the supernatant from step 1 to 90°C, adjust the pH value to 4.5 with citric acid, set the temperature at 40°C, and stir at a speed of 40r / min to precipitate the protein for 20min, then centrifuge at 3000r / min for 10min, and remove the supernatant Liquid, collect the solid phase, add water and mix according to the above steps again, so that the protein precipitates at the isoelectric point, repeat three times, finally wash twice with water, and centrifuge to obtain the solid phase, which is rapeseed protein, weighing 589g;

[0065] (3) Pour the precipitate in step 2 into 6L water, heat to 90°C, keep for 30min, then cool to 50°C, add 29.45g of neutral protease As1.398 (enzyme activity is 130000U / g), adjust with NaOH reagent and Keep th...

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Abstract

The invention provides a method for preparing a rapeseed bioactive peptide, comprising the following steps: taking degreased rapeseed dregs as a raw material, preparing a rapeseed protein by an alkali dissolving and acid precipitation method; adding protease hydrolyzed proteins; carrying out activated carbon decoloration, ultrafiltration grading, and cation-anion exchange resin desalination in sequence; and finally concentrating, spray drying or freeze drying to obtain the rapeseed bioactive peptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing rapeseed biologically active peptides by using rapeseed meal as a raw material and adopting a protease hydrolysis process. Background technique [0002] Rapeseed contains 20% to 25% protein, and defatted rapeseed meal contains 32% to 40% protein. Rapeseed protein is mainly composed of 12S protein with high relative molecular mass and 2S protein with low relative molecular mass. Rapeseed protein is a high-quality protein. Its amino acid composition conforms to the recommended model value of FAO / WHO, and its PER value is higher than that of other plants. Protein, even higher than casein, is characterized by a higher content of sulfur-containing amino acids and lysine. Rapeseed protein not only has good nutritional properties, but also its functionality is superior to other proteins, especially its oil absorption, water retention and emulsification pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/10C07K1/36C07K1/34C07K1/18C07K1/14
Inventor 陈燕飞亓培实何东平刘良忠何莉萍江思佳黄迪惠倪倩刘雪姣宋洋
Owner 武汉百信正源生物技术工程有限公司
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