Au NPs-CeO2@PANI (polyaniline) nanocomposite, preparation method thereof and glucose biological sensor manufactured by utilizing nanocomposite

A nanocomposite material and biosensor technology, applied in the field of glucose biosensors, can solve problems such as restricting use, and achieve the effects of maintaining biological activity, high sensitivity, and broadening the linear range

Inactive Publication Date: 2011-09-07
甘肃省科学院传感技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the redox reaction of polyaniline can only be carried out efficient

Method used

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  • Au NPs-CeO2@PANI (polyaniline) nanocomposite, preparation method thereof and glucose biological sensor manufactured by utilizing nanocomposite
  • Au NPs-CeO2@PANI (polyaniline) nanocomposite, preparation method thereof and glucose biological sensor manufactured by utilizing nanocomposite
  • Au NPs-CeO2@PANI (polyaniline) nanocomposite, preparation method thereof and glucose biological sensor manufactured by utilizing nanocomposite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of Au NPs-CeO 2 @PANI Nanocomposites,

[0038] A, preparation of cerium dioxide nanoparticles and gold nanoparticles;

[0039] Take 30mL 0.15mol / L Ce(NO 3 ) 3 solution, add 6mL3%H 2 o 2 , and then add 2mol / L ammonia water while stirring to form a yellow precipitate, until the pH value is greater than 9, so that the precipitation is complete. The pellet was centrifuged twice for about 15 min each time. Add dilute HNO to the precipitate 3 (1mol / L) to adjust the pH to 1.4, and peptize at a water bath temperature of 60°C to form light yellow and transparent CeO 2 Hydrosol. CeO 2 The sample obtained after the water sol was evaporated to dryness was calcined at 550 °C for 2 h to obtain nano-CeO 2 powder;

[0040] Add 3 mL of trisodium citrate solution (0.034 mol / L) into a three-necked flask filled with 100 mL of water, heat to boiling, quickly inject 1 mL of chloroauric acid solution (0.024 mol / L) under stirring, and adjust the heat source so...

Embodiment 2

[0045] Example 2: Using Au NPs-CeO 2 @PANI nanocomposite immobilized glucose oxidase to make glucose biosensor.

[0046] (1) Platinum base electrodes with a diameter of 2 mm are made of 0.3 and 0.5 μm Al 2 o 3 After the polishing powder is polished into a mirror surface, it is ultrasonically cleaned with acetone, absolute ethanol and deionized water for 5 minutes in sequence;

[0047] (2) At 0.5% H 2 SO 4 Electrochemical pretreatment is carried out in (1), and the substrate electrode treated in (1) is scanned by cyclic voltammetry at a scan rate of 50mV / s for 30 cycles at -0.4~+1.0V until it is stable;

[0048] (3) The prepared Au NPs-CeO 2 @PANI was dissolved in deionized water at a concentration of 1mg / mL, sonicated for 1h; take 3μL Au NPs-CeO 2 Mix @PANI with 3 μL 1mg / μL glucose oxidase (GOD) and 1 μL 1.0% chitosan (Chit) thoroughly, then drop on the surface of the platinum disk electrode treated by the above (2) step, and let it dry at room temperature, so that A gl...

Embodiment 3

[0062] Example 3: Preparation of Au NPs-CeO 2 @PANI Nanocomposites,

[0063] Take 0.206g CeO 2 Ultrasonic dispersion of nanoparticles in 20mL 1 mol L -1 In the HCl solution, add 1 mL of An (CeO 2 The molar ratio of An to An was 1:0.5), and ultrasonically dispersed for 30 min. Other preparation steps are the same as in Example 1. The catalytic behavior of the modified electrode on glucose oxidase was evaluated by cyclic voltammetry, as shown in figure 1 (b). Compared figure 1 (a), (b), the peak current in (b) is significantly lower than that in (a), because of the excess CeO 2 The addition of , the conductivity of the composite material is reduced, thereby reducing the catalytic ability of glucose oxidase to glucose.

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Abstract

The invention discloses an Au NPs-CeO2@PANI nanocomposite, a preparation method thereof and a glucose biological sensor manufactured by utilizing the nanocomposite. Firstly, the Au NPs-CeO2@PANI nanocomposite is composited by utilizing cerium dioxide (CeO2) nano particles, aniline (An) monomers and aurum (Au) nano particles; and the Au NPs-CeO2@PANI nanocomposite is fixed with glucose oxidase, soas to manufacture the glucose biological sensor. The nanocomposite prepared in the invention has a good compatibility and is favourable for fixing bioenzyme; the cost of the preparation method is low, and the preparation method is simple and fast. The biological sensor manufactured in the invention has the advantages of high sensitivity, good stability and wide linear range, and has a certain capacity for resisting interference, therefore the biological sensor can be widely used in the fields of diabetes diagnosis and food process monitoring. The linear range for detecting diabetes is 6.2*10<-6> to 2.8*10<-3>M, and the detection limit is 1.0*10<-6>M.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and its production, specifically a kind of Au NPs-CeO 2 @PANI nanocomposite material and its preparation method and glucose biosensor made of this material. Background technique [0002] In recent years, with the development of nanoscience and the continuous development of nanomaterial preparation technology, nanomaterials have been widely used in the development of biosensors due to their unique physical and chemical properties. Novel nanomaterials to enhance the electrochemical performance of biosensors. So far, numerous nanomaterials such as precious metals such as Au, Ag, and Pt, carbon nanotubes, conductive polymer films, etc. have been successfully used to immobilize glucose oxidase. Among these nanomaterials, conductive polymers have become one of the hot materials that scientists pay attention to in the field of biosensor research due to their advantages of effective e...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘国汉韩根亮马莉萍左显维王艳凤郑礡
Owner 甘肃省科学院传感技术研究所
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