HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties

A technology for apples and varieties, which is applied in the field of establishing identification methods to achieve reliable experimental results and reduce oxidation.

Inactive Publication Date: 2011-09-07
山东省农业科学院中心实验室
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic regional variety identification mainly focuses on the rapid characterization of tobacco leaves by direct point spray ion trap mass spectrometry (Liu Wei et al., 2008) or the identification and characterization of different varieties or differe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties
  • HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties
  • HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] The technical solution of the present invention will be further described below in conjunction with the examples.

[0037] 1. Instruments and samples

[0038] 11. Instrument: Chromatographic column: Atlantis C18 (5μm, 250mm×4.5mm)

[0039] 1.2. Samples: Seven apple varieties are Red Fuji, Gala, Red Delicious, American No. 8, Golden Marshal, Green Apple, and Red Rose; test the same variety of Red Fuji, Gala and American No. 8 from different origins, such as The differences between Yantai, Yiyuan, Fujian, New Zealand, and the United States were compared.

[0040] 2. Methods and Results

[0041] In the optimization of sample pretreatment and extraction methods, seasonal Gala apples were selected as experimental samples.

[0042] 2.1. Optimizing the pretreatment of samples. The main factors affecting browning are oxygen and temperature. In order to effectively prevent the browning reaction of polyphenols in apples during the experiment, three schemes were adopted in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an HPLC (high performance liquid chromatography) method for synchronously detecting five polyphenols in apples and distinguishing varieties. The method comprises the following steps: removing the core of an apple; adding ascorbic acid; preventing browning by virtue of an ultrasound, nitrogen protection or heating method after the core is crushed; adding a methanol-water solution or ethyl acetate for ultrasonic extraction; collecting the supernatant and extracting once again; combining the supernatants; and performing HPLC detection. Chlorogenic acid and rutin are used as target compounds for distinguishing seven varieties of apples according to content analysis of the measured five polyphenols of different varieties and different regions. The method disclosed by the invention has the advantages that the five polyphenols are separated and measured simultaneously and rapidly by virtue of PHLC, and the purpose of distinguishing different apple varieties is achieved; the pre-processing of the method is simple; the cost is low; the measurements can be performed simultaneously; and the distinguishing for different apple varieties is realized.

Description

technical field [0001] The invention relates to a method for detecting five kinds of polyphenols in apples, in particular to the establishment of a method for identifying different varieties by using the detected content of polyphenols. Background technique [0002] Polyphenols are a general term for substances with benzene rings and multiple hydroxyl chemical structures, including flavonoids, tannins, phenolic acids, and anthocyanins. Generally, as a secondary product in growth metabolism, it is widely present in plant tissues, and it plays a role in the growth and regulation of plants, the induction of gene expression, signal transduction, the absorption of ultraviolet radiation, biological nitrogen fixation, and the formation of flavor during fruit and vegetable processing. , color changes (such as browning), turbidity and precipitation of fruit and vegetable beverages, etc. have certain influences. Among them, gallic acid, chlorogenic acid, caffeic acid, protocatechuic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/06G01N30/34
Inventor 吕潇赵善仓郭长英邓立刚谷小红陈宁宁李增梅苑学霞陈学兵
Owner 山东省农业科学院中心实验室
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap