Preparation method of polysaccharide composite with antitumor activity
A technology of anti-tumor activity and composition, which is applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of wild Cordyceps, Phellinus linden resources, and anti-tumor activities that need further research, Fruiting body spores can not be formed in large quantities, etc., to achieve the effect of enhancing anti-tumor activity, product safety and reliability, and strong professionalism
Active Publication Date: 2012-08-08
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF1 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] Due to the increasing demand for Cordyceps and Phellinus in domestic and foreign markets, the pesticide farmers in various domestic production areas have predatory collection, and the fruiting body spores cannot be formed in large quantities. As a result, wild Cordyceps and Phellinus resources are hard to find in my country and will soon be exhausted.
In recent years, a large number of scientific researchers have carried out extensive research on the artificial cultivation of Cordyceps and Phellinus Phellinus, liquid fermentation technology, etc. for further study
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0022] (1) Artificially cultured fruiting bodies of Cordyceps: You can buy them at major Chinese medicine stores.
[0023] (2) Phellinus mycelia cultured by liquid fermentation: For the method of cultivating Phellinus mycelium, refer to the invention patent of our research laboratory in the early stage "a medium and cultivation method for high-efficiency liquid culture of Phellinus mycelium (application number: 201010255665.9 )".
[0024] Specifically: 1L of fermentation medium, the concentration (g / L) of each component is: maltose 30g, yeast powder 20g, MgSO 4 1.0g, KH 2 PO 4 1.0g, triammonium citrate 1.0g, vitamin B 1 8mg, vitamin B 2 4mg, vitamin B 6 0.52mg, niacinamide 26mg, calcium pantothenate 2.6mg, water extract of Mori twig 5g. Divide the culture medium into 50mL shake flasks, the filling volume is 30mL, the culture medium sterilization temperature: 121°C, sterilization time: 30min;
[0025] Mycelium culture: the Phellinus bacterium classification culture t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a preparation method of a polysaccharide composite with antitumor activity, which comprises the steps of: drying and crushing artificially-cultured worm grass sporocarp and liquid-fermentation cultured phellinus igniarius, adding distilled water for extraction and alcohol precipitation centrifugation, dissolving into water, and filtering under high pressure by using an ultrafiltration membrane to obtain worm grass sporocarp polysaccharide and phellinus igniarius polysaccharide; and uniformly mixing the worm grass sporocarp polysaccharide with the phellinus igniarius polysaccharide according to the ratio and pelleting through a pelleting machine. The invention has the advantage that the worm grass sporocarp polysaccharide and the phellinus igniarius polysaccharide are compounded, thus the antitumor activity can be enhanced by using mutual cooperation of two kinds of polysaccharides. The preparation method has the advantages of simple process, strong professionalof the established extraction method and good repeatability; and the polysaccharide composite has the advantages of environment friendliness due to the adoption of extraction with medical alcohol, safety and reliability, and is suitable for industrialized production.
Description
technical field [0001] The invention relates to a preparation method of a polysaccharide composition with anti-tumor activity. The product prepared by the method of the invention has the effect of directly inhibiting the growth and proliferation of tumor cells, and can also improve the immunity of the body. It can be used as a drug for auxiliary treatment of tumors or Health products. Background technique [0002] Since the 1960s, fungal polysaccharides have attracted great interest as broad-spectrum immune enhancers against tumors. A large number of pharmacological and clinical studies have shown that fungal polysaccharides have complex and multifaceted biological activities and functions, and have no toxic side effects on normal cells. Fungal polysaccharides mainly affect the reticuloendothelial system, macrophages, lymphocytes, white blood cells and proteins. The synthesis, antibody production and induction of interferon can improve the body's immune function and enhance...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/068A61P35/00
Inventor 计东风李有贵钟石吕志强胡桂燕林天宝朱燕
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES