Supercharge Your Innovation With Domain-Expert AI Agents!

Fluorescence detection method of potassium ion

A technology for fluorescence detection and potassium ions, which is applied in the field of fluorescence detection of potassium ions, can solve the problems of high detection cost, limited practical application, low sensitivity and selectivity, and achieve high sensitivity, save detection time, and simplify operation steps.

Inactive Publication Date: 2011-09-14
LUDONG UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, methods for detecting potassium ions based on conformational changes of nucleic acid aptamers have been reported [UEYAMA H, TAKAGI M, TAKENAKA S.A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with guanine quartet-potassium ion complex formation[J].J Am Chem Soc,2002,124(48):14286-14287.HO H A, LECLERC M.Optical sensors based on hybrid aptamer / conjugated polymer complexes[J].J Am Chem Soc,2004,126( 5): 1384-1387.], but there are still shortcomings of low sensitivity and selectivity, and some need to carry out DNA double labeling, and the detection cost is high, which limits its practical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence detection method of potassium ion
  • Fluorescence detection method of potassium ion
  • Fluorescence detection method of potassium ion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Add 2mL buffer (50mM Tris-HCl, pH 7.40) to the cuvette, add 5μL 4×10 -5 Mthiazole orange, 5 μL 6 × 10 -5 M Potassium ion-specific DNA and 5 μL 0.4M potassium chloride were mixed evenly and reacted at 25°C for 4 minutes, and the fluorescence test was performed. At this time, the final concentration of each substance was: [thiazole orange]=100 nM, [potassium ion-specific DNA]=150 nM, [potassium ion]=1 mM.

[0030] The fluorescence spectra before and after the addition of potassium ions are shown in figure 1 As shown, before the addition of potassium ions, the fluorescence intensity at the maximum emission wavelength is 353.06, and after the addition of potassium ions, the fluorescence intensity at the maximum emission wavelength is 151.42, so the present invention can quantitatively detect potassium ions.

Embodiment 2

[0032] Potassium ions are replaced by other metal cations such as calcium ions, magnesium ions, lithium ions, ammonium ions, and sodium ions repeat the steps of Example 1, and the results are as follows figure 1 shown. Calcium ions, magnesium ions, lithium ions, ammonium ions, and sodium ions can also reduce the fluorescence intensity, but compared with potassium ions, the reduction is very small. It shows that the present invention is less interfered by other metal cations and has better selectivity.

Embodiment 3

[0034] Change the potassium ion concentration into the potassium ion of different concentrations and repeat the steps of Example 1, the results are as follows figure 2 shown. It shows that the present invention can quantitatively detect potassium ions of different concentrations. When [thiazole orange]=100nM, [potassium ion-specific DNA]=150nM, the concentration of potassium ion is proportional to the fluorescence intensity in the range of 1-60μM and 400-900μM.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fluorescence detection method of a potassium ion. The specific DNA (5'-GGGTTAGGGTTAGGG TTAGGG-3') of the potassium ion is in an irregular curling state when no potassium ion exists, fluorescent dye thiazole orange and a G-rich sequence are acted to generate very strong fluorescence, after the potassium ion is added, the specific DNA of the potassium ion forms a G tetrahedral structure, and the fluorescence is decreased. The fluorescence detection method disclosed by the invention not only has high sensitivity and better specificity, but also is simple and rapid, and the whole process can be completed in five minutes.

Description

technical field [0001] The invention relates to the detection field of potassium ions, in particular to a fluorescence detection method for potassium ions. Background technique [0002] Potassium is an indispensable constant element in the human body, and its main function is to maintain the normal function of nerves and muscles. K + It can participate in biological processes such as nerve impulse transmission and cell activity regulation. The content of potassium ions is an important indicator of human physiological activities, and the level in blood and urine is particularly important for clinical diagnosis [KOFUJI P, NEWMAN E A. Potassium buffering in the central nervous system [J]. Neuroscience, 2004, 129: 1045-1056.], so the determination of potassium ion content is of great significance. At present, there are many methods for potassium ion detection, such as flame photometry, ion selective electrode method, atomic absorption spectrometry, potentiometric titration, et...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 徐慧陈建农高淑丽柳全文
Owner LUDONG UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com