Preparation method for drug sensitivity kit and drug sensitivity detection method
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A kit and drug-sensitivity technology, applied in biochemical equipment and methods, microbial measurement/inspection, specific-purpose bioreactor/fermenter, etc., can solve the problems of ineffective detection, limited virus and bacteria, etc. , to achieve the effect of low cost, protection of ecological environment and easy operation
Inactive Publication Date: 2011-09-28
NINGBO ACAD OF OCEAN & FISHERY
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Since the virus and bacteria are not cultivated in this kind of detection box, the virus and bacteria obtained are limited, so it is impossible to carry out effective detection at all.
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[0032] The invention relates to a method for preparing an aquaculture drug-sensitivity kit. The drug-sensitivity kit includes a centrifuge tube filled with a culture solution for enrichment, a petri dish with a solid medium and a drug-sensitivity storage ring. The drug sensitivity kits are further divided into freshwater type and seawater type, wherein the freshwater type drug sensitivity kit is used for drug sensitivity detection in freshwater aquaculture, and the seawater type drug sensitivity kit is used for drug sensitivity detection in seawater aquaculture. sensitive detection.
[0033] The preparation method of the fresh water type drug susceptibility kit.
[0034] Preparation of centrifuge tubes filled with enrichment medium:
[0035] a. Pour 5-15 parts of peptone, 3-8 parts of yeast extract, and 5-15 parts of sodium chloride into a beaker filled with 600-1000 parts of distilled water, and stir evenly.
[0036] b. Pour hydrochloric acid or sodium hydroxide into the be...
Embodiment
[0053] 1) Weigh 10g of peptone, 5g of yeast extract, and 10g of sodium chloride, pour them into a beaker filled with about 800ml of distilled water, and stir evenly. Use hydrochloric acid or sodium hydroxide to adjust the pH to 7.3±0.1, then distill the volume to 1000ml with distilled water to prepare a culture medium for enrichment.
[0054] Put the above enrichment culture solution into the adjustable dispenser, add 0.5ml of the enrichment culture solution to each centrifuge tube, screw the cap on gently but not tightly, leaving about half a circle or one circle.
[0055] Finally, put the centrifuge tubes containing the enrichment culture solution into stainless steel test tube racks respectively, and then put them into an automatic autoclave sterilizing pot at 0.102 MPa (121° C.), autoclaving for 15 minutes.
[0056] After the sterilization is finished, take out the centrifuge tube, tighten the cap of the centrifuge tube, and put it into a vacuum packaging bag for vacuuming...
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Abstract
The invention relates to a preparation method for a drug sensitivity kit and a drug sensitivity detection method, which are applied in the field of aquiculture. The drug sensitivity kit comprises a centrifuge tube with enriched bacterial culture solution, a culture dish with solid culture medium and a drug sensitivity drug storage ring. According to the invention, drug sensitivity detection is carried out on suscepts and water samples and the drug sensitivity drug storage rings are observed after the detection; drugs in drug sensitivity plasters where a transparent area appears can all be used as alternative drugs, and the drug in a drug sensitivity plaster where a big clear area appears can be used as a preferable drug; therefore a most appropriate antibiotic is selected out. Using a drug based on a correct diagnosis can avoid abuse of fish drugs and particularly abuse of antibiotics, and prevent medicinal residues from remaining in aquatic products. It only needs 12 hours to complete the preparation of the drug sensitivity kit; thus selection of drugs and corresponding prevention and treatment can be carried out in a same day as the preparation. The invention has the advantages of simple and convenient operation and low cost, is applicable to selection of drugs for controlling bacterial diseases in basic level of aquiculture, and plays a positive role in protecting ecological environment for aquiculture.
Description
technical field [0001] The invention relates to a drug sensitivity kit and a drug sensitivity detection method for aquaculture, in particular to a preparation method of a drug sensitivity kit for detecting disease bodies or water samples in freshwater aquaculture or seawater aquaculture, and a drug sensitivity detection method. Detection method. Background technique [0002] Due to excessive breeding density, environmental deterioration and other reasons, bacterial diseases in aquaculture have occurred frequently in recent years, causing huge economic losses. Due to the lack of scientific knowledge of farmers and the drive of market economic interests, the abuse of fishery drugs, especially antibiotics, is widespread, leading to increasing problems such as drug resistance of aquatic pathogens, drug residues in aquatic products, environmental pollution and ecological damage. To solve the problem of antibiotic abuse from the source, the key is to conduct a drug susceptibility...
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