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Method for extracting and separating soybean isoflavone monomer compounds from soybeans

A technology of soybean isoflavones and extracts, applied in the direction of organic chemistry, can solve the problems of troublesome operation and low purity of ingredients, and achieve the effect of improving purity and efficiency, high content and high efficiency

Inactive Publication Date: 2013-10-30
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned various methods may adopt multi-step separation, and the operation is cumbersome; or only one or a few soybean isoflavone components can be obtained; or the obtained components are not high in purity; or chloroform toxic solvent is used in the separation process

Method used

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  • Method for extracting and separating soybean isoflavone monomer compounds from soybeans
  • Method for extracting and separating soybean isoflavone monomer compounds from soybeans
  • Method for extracting and separating soybean isoflavone monomer compounds from soybeans

Examples

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Embodiment

[0029] Example: 1000 grams of defatted soybean meal was soaked in 60% ethanol for 8 hours, and extracted by percolation with 10 times the volume of 60% ethanol. During the percolation extraction, a double-layer percolation column with jacket heating and heat preservation was used, the medium temperature was 50°C, and the percolation liquid flow rate was 3 times the column volume / hour. The percolation liquid is recovered to be alcohol-free and then spray-dried to obtain coarse extract powder, which is refluxed with 90% acetone for extraction, concentrated and crystallized to obtain soybean isoflavone extract.

[0030] Soybean isoflavone extracts were dissolved in methanol, subjected to Superose 12 chromatography, and methanol-water was used as the eluent for elution. The flow rate of the eluent was 5 times the column volume per hour, and the effluent was detected by a UV detector at 254 nm. The fractions of the target components are collected, and the obtained fractions are c...

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Abstract

The invention discloses a method for extracting and separating soybean isoflavone monomer compounds from soybeans. The steps include (1) preparation of soybean isoflavone extract and (2) column chromatography purification. The soybean isoflavone extract obtained by the method has high content of soybean isoflavones and low impurities, which is beneficial to subsequent further purification; in the purification process, organic solvents such as chloroform, which are harmful to the environment, are not used, and are environmentally friendly; a simple and convenient method is established. , efficient, and a way to achieve the purpose of the invention, the conditions of the chromatography method are optimized, and the purity and efficiency are greatly improved.

Description

technical field [0001] The invention belongs to the field of chemical industry, and specifically relates to a method for extracting and separating various high-purity soybean isoflavone monomer compounds from soybeans, mainly for separating and purifying glycidin (Glycitin), daidzin (Daidzin), and genistin ( Genistin), Glycitein, Daidzein, Genistein and other compounds. Background technique [0002] Soy isoflavones are secondary metabolites formed during soybean growth and are a mixture of polyphenols. The various nutritional and health functions of soybeans are related to soybean isoflavones. Modern pharmacological studies have shown that soybean isoflavones have estrogen-like effects and can prevent menopausal syndrome in women; they can prevent and improve osteoporosis, reduce bone loss, increase the body's absorption of calcium, and increase bone density; It has obvious therapeutic effects on breast cancer, colon cancer, lung cancer, prostate cancer, skin cancer and le...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/12C07D311/40C07D311/36
Inventor 孙爱玲柳仁民
Owner LIAOCHENG UNIV