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Method for extracting natural antioxidant from shells of camellia oleifera

A natural anti-oxidation and Camellia oleifera husk technology, which is applied in anti-toxic agents, drug combinations, and pharmaceutical formulas, can solve the problems of low utilization rate of Camellia oleifera husk and limited types of raw materials for proanthocyanidin extraction, and achieve strong antioxidant capacity and long extraction time. Short, efficient use of the effect

Active Publication Date: 2011-10-12
WUFENG CHICHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the shortcomings of low utilization rate of camellia oleifera husk and limited types of raw materials for proanthocyanidin extraction in the prior art, the present invention provides a method for extracting natural antioxidant substances from camellia oleifera husk, which can increase the added value of camellia oleifera husk , to expand the source of raw materials for proanthocyanidins

Method used

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  • Method for extracting natural antioxidant from shells of camellia oleifera
  • Method for extracting natural antioxidant from shells of camellia oleifera
  • Method for extracting natural antioxidant from shells of camellia oleifera

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for extracting natural antioxidant substances from camellia oleifera shell:

[0034] (1) Pulverization: Pulverize the purified Camellia oleifera husk raw material with a plant pulverizer (sieve hole Φ=2-4 mm).

[0035] (2) Extraction: using water as solvent, using microwave extraction, the solid-liquid mass volume ratio is 1:15-25, controlling the microwave irradiation temperature at 60-80°C, and the irradiation time for 15-35 min, to obtain the extract; the temperature can be Yes: 60°C, 65°C, 70°C, 75°C, 80°C.

[0036] (3) Centrifugation: The extract was centrifuged at 5000 rpm for 30 min in a centrifuge to separate the supernatant and discard the precipitate.

[0037](4) Resin adsorption separation: put the pre-purified non-polar or low-polar macroporous adsorption resin on the above supernatant, the flow rate of the upper column liquid is 1.4-1.7 Bv / h, and wash the column with pure water after adsorption. Then elute with ethanol aqueous solution with a vol...

Embodiment 2

[0041] (1) Put 50 g of Camellia oleifera husk raw material that has been purified, air-dried and crushed (using a plant pulverizer to pass through a Φ2 mm sieve plate) and 750 mL of pure water into a 1000 mL three-necked flask for microwave extraction and controlled microwave irradiation The temperature was 80°C, and the irradiation time was 35 min to obtain 620 mL of extract.

[0042] (2) After the extract was cooled, it was centrifuged in a centrifuge, set the speed of the centrifuge at 5000 rpm, centrifuged for 30 min, and separated 550 mL of supernatant.

[0043] (3) Add the above supernatant to the pretreated AB-8 type macroporous resin (Bv=265 cm 3 ), and then washed the column with 2.4 Bv / h of pure water, and then eluted with 600 mL of 70% ethanol-water solution at a flow rate of 1.7 Bv / h, and combined the eluents.

[0044] (4) Under normal pressure (1.01×10 5 Pa) Ethanol in the ethanol-water eluent is distilled off (recycled) to obtain a liquid natural antioxidant s...

Embodiment 3

[0047] (1) Put 30 g of Camellia oleifera husk raw material that has been purified, air-dried and crushed (using a plant grinder through a Φ2 mm sieve plate) and 750 mL of pure water into a 1000 mL three-neck flask for microwave extraction and controlled microwave irradiation The temperature was 60°C, and the irradiation time was 35 min to obtain 650 mL of extract.

[0048] (2) After the extract was cooled, it was centrifuged in a centrifuge, set the centrifuge speed at 5000 rpm, centrifuged for 30 min, and separated 580 mL of supernatant.

[0049] (3) Add the above supernatant to the pretreated AB-8 type macroporous resin (Bv=265 cm 3 ), then wash the column with 2.4 Bv / h of pure water, and then elute with 500 mL of 70% ethanol aqueous solution at a flow rate of 1.5 Bv / h, and combine the eluents.

[0050] (4) Under normal pressure (1.01×10 5 Pa) Ethanol in the ethanol-water eluate is evaporated (recycled), and the liquid natural antioxidant substance whose main component is...

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Abstract

The invention relates to a method for extracting a natural antioxidant from shells of camellia oleifera. With crushed shells of the camellia oleifera as raw materials and water as a solvent, the method comprises the following steps of: extracting by a microwave-assisting extract method to obtain an extracting solution, carrying out centrifugal separation on the extracting solution to obtain a supernatant, separating the supernatant by using a nonpolar or low-polarity macroporous adsorption resin, carrying out elution on the solution by using an aqueous solution of alcohol, and distilling the alcohol from an eluent to obtain a liquid natural antioxidant with procyanidins of the shells of the camellia oleifera as a main components; and carrying out freeze-drying on the obtained liquid natural antioxidant to obtain powdery natural antioxidant. The target product prepared by the method disclosed by the invention has stronger anti-oxidation biological activity.

Description

technical field [0001] The invention relates to a method for extracting natural antioxidant active substances from plant materials, in particular to a method for extracting natural antioxidant substances from camellia oleifera shells. Background technique [0002] Camellia oleifera is a unique woody edible oil tree species in my country, and it is also one of the world's four largest woody edible oil source tree species with the same reputation as oil palm, olive and coconut. There are nearly 50 million mu of camellia oleifera forest in China, with an annual output of about 1 million tons of camellia oleifera seeds, which can produce edible camellia oil with rich nutritional value. Camellia oleifera husk (also known as camellia oleifera, the peel of camellia oleifera fruit) accounts for about 60% of the weight of the whole camellia oleifera fruit, and is the residue of the camellia oleifera processing industry. According to estimates, its annual production is about 1.6 milli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P39/06A61K131/00
Inventor 张亮亮汪咏梅吴冬梅徐曼陈笳鸿
Owner WUFENG CHICHENG BIOTECH
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