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Concrete flavouring for tobacco and preparation method and application thereof

A technology for tobacco spices and extracts, which is applied in the field of tobacco additives, can solve the problems of few comprehensive utilization channels, occupy a large storage space, and low comprehensive utilization degree, achieve strong standardization and controllability, improve utilization rate, The effect that is conducive to the benign development

Active Publication Date: 2011-10-19
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few ways to comprehensively utilize these wastes, and the degree of comprehensive utilization is very low. Most of them are discarded as waste, causing great pollution to the environment.
In addition, domestic cigarette companies currently store more upper tobacco leaves, which are generally difficult to use in leaf group formulas due to their high nicotine content, which not only takes up a lot of storage space, but also causes a lot of waste

Method used

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  • Concrete flavouring for tobacco and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] ——Preparation of Tobacco Flavor Extract 1

[0028] 1. Medium and Culture Conditions

[0029] The strains used in the experiment were inoculated into 50 mL of LB liquid medium, and cultured at 37 °C for 12 h to obtain liquid strains. The liquid strains were transferred to 1 L of LB liquid medium according to the inoculum amount of 5%, and cultured with shaking at 37°C and 150 r / min for 12 h. Centrifuge the culture solution at 3500 rpm / min for 10 min, wash the precipitate with sterile water, centrifuge, and finally shake it evenly with 20 mL of sterile water, and dilute it 10 times before use to obtain the bacterial agent.

[0030] 2. Fermentation treatment of waste tobacco leaves

[0031] 1) Fermentation by nicotine-degrading bacteria

[0032] For every 200 g of low-grade tobacco leaves, 40 mL of nicotine-degrading bacteria (Rhodococcus Y22) bacterial agent was sprayed (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf sa...

Embodiment 2

[0052] ——Preparation of Tobacco Flavor Extract 2

[0053] 1. Culture medium and culture conditions: the same as in Example 1.

[0054] Fermentation treatment of waste tobacco leaves

[0055] 1) Fermentation by nicotine-degrading bacteria

[0056] For every 200 g of low-grade tobacco leaves, 40 mL of nicotine-degrading bacteria (Rhodococcus Y22) bacterial agent was sprayed (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf samples were fermented in a 22°C, 60% constant temperature and humidity box for 10 days for the next experiment.

[0057] 2) Mixed fermentation treatment of esterase-producing bacteria and aroma-producing bacteria

[0058] The samples fermented by nicotine-degrading bacteria were sprayed with 20 mL of ferulic esterase-producing bacteria (Bacillus pumilus Van35) and aroma-producing bacteria (Bacillus subtilis) (the control sample was sprayed with an equal volume of sterile water). The treated tobacco leaf samp...

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Abstract

The invention discloses concrete flavouring for tobacco and a preparation method and application thereof, belonging to the technical field of additive for tobacco. In the invention, waste tobacco used as raw material is sequentially fermented with rhodococcus, bacillus pumilus and bacillus subtilis to reduce nicotine in the waste tobacco and convert ferulic acid in the tobacco into incense ingredients such as 4-guaethol and vanillin; after fermentation is finished, the conventional biochemical technology is utilized to extract the incense ingredients from the waste tobacco for preparing concrete flavouring. The concrete flavouring obtained by invention contains the following main incense ingredients with higher contents: benzyl alcohol, solanone, vanillin, BETA-damascenone, dihydroactinidiolide, megastigmatrienone, duvatriene diglycol and neophytadiene. Results of an aromatic flavouring smoke panel test show that the concrete flavouring has obvious effects of increasing tobacco aroma,smoothing alcohol and smoke, reducing irritation, covering impurity gas and improving smoking taste of cigarettes.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, and more specifically, the invention relates to a flavor extract for tobacco. At the same time, the invention also relates to a preparation method of the spice extract and its application in the tobacco industry. Background technique [0002] Tobacco is one of the important pillars of our country's finances. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overcome the key technologies that affect the aroma and quality of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competitiveness. . [0003] In the process of tobacco planting and processing, a large amount of tobacco waste such as low-grade tobacco leaves, tobacco stems and tobacco dust is produced, accounting for 10% to 25% of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B3/12C11B9/00
Inventor 段焰青者为杨金奎叶灵张克勤汤丹瑜廖头根
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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